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Method for quickly breeding Ilex dabieshanensis K Yao.et M.P.Deng in vitro

A technology of holly and explants, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve problems such as in vitro rapid propagation research that has not yet been reported, and achieve the effect of efficiently obtaining and solving resource shortages

Inactive Publication Date: 2011-04-20
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

And about the research on Ilex plant tissue culture, there are few reports at home and abroad.
In the past 5 years, there have only been reports on holly (Ilex chinensis Sims) (see Wenzhou Agricultural Science and Technology, No. 4, 2007, tissue culture and rapid propagation technology of holly), and American holly [Ilex verticillata (Linn.) A.Gray] (see plant physiology communication, the 2nd issue in 2007, tissue culture and rapid propagation of American holly) for tissue culture, but there is no report yet about the in vitro rapid propagation research of Dabieshan holly 1#

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] 1. Select 50 robust Dabie Mountain holly 1# annual cutting seedlings. Cut the young shoots of the year, remove the leaves, and use the stem segments as explants, each stem segment has an axillary bud, about 2cm long. Rinse the explants with clean water, rinse once with washing powder, and sterilize with 84 disinfectant on a shaking table for 15 minutes. After rinsing with running water for 20 minutes, sterilize with 75% ethanol for 50 seconds on a sterile operating table, immediately pour 0.1% mercuric chloride for 10 minutes, and finally wash with sterile water 4 times, shaking for 3 minutes each time. Both ends of the sterilized explant were excised by 0.5 cm to remove the injured part. Access induction medium MS+6-BA0.5mg / L+KT0.05mg / L+NAA0.05mg / L+GA 3 0.1mg / L. Each bottle received 3 plants, a total of 50 bottles were connected. Under the condition of temperature 24°C, light time 10h / day, and light intensity 2000lx, culture was carried out.

[0018] 2. Seven days...

Embodiment 2

[0023] 1. Select 30 robust Dabie Mountain Holly 1# annual cutting seedlings. Cut the aboveground part, remove the leaves, and use the stem segment as an explant, each stem segment has an axillary bud, about 2cm long. Rinse the explants with clean water, rinse once with washing powder, and sterilize with 84 disinfectant on a shaking table for 18 minutes. After rinsing with running water for 25 minutes, sterilize with 75% ethanol for 55 seconds on a sterile operating table, immediately pour 0.1% mercuric chloride for 10 minutes, and finally wash with sterile water 4 times, shaking for 4 minutes each time. Both ends of the sterilized explant were excised by 0.5 cm to remove the injured part. Access induction medium MS+6-BA0.5mg / L+KT0.05mg / L+NAA0.05mg / L+GA 3 0.1mg / L. Each bottle received 3 plants, a total of 50 bottles were connected. Under the condition of temperature 25°C, light time 16h / day, and light intensity 1600lx, culture was carried out.

[0024] 2. The axillary buds...

Embodiment 3

[0029] 1. Select 40 robust Dabie Mountain holly 1# annual cutting seedlings. Cut the aboveground part, remove the leaves, and use the stem segment as an explant, each stem segment has an axillary bud, about 2cm long. Rinse the explants with clean water, rinse once with washing powder, and sterilize with 84 disinfectant on a shaker for 20 minutes. After rinsing with running water for 30 minutes, sterilize with 75% ethanol for 60 seconds on a sterile operating table, immediately pour 0.1% mercuric chloride for 10 minutes, and finally wash with sterile water 4 times, shaking for 5 minutes each time. Both ends of the sterilized explant were excised by 0.5 cm to remove the injured part. Access induction medium MS+6-BA0.5mg / L+KT0.05mg / L+NAA0.05mg / L+GA 3 0.1mg / L. Each bottle received 3 plants, a total of 50 bottles were connected. Under the condition of temperature 26°C, light time 16h / day, and light intensity 1600lx, culture was carried out.

[0030] 2. The axillary buds began ...

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Abstract

The invention provides a method for breeding Ilex dabieshanensis K Yao.et M.P.Deng in vitro through tissue culture, comprising the following steps of sterilizing an explant, inoculating the sterilized explant to an inducing culture medium for differentiation inducing of buds; controlling the culture temperature to be 20-26DEG C; culturing in a dark environment for 20-26h and then normally culturing at the illumination time of 10-16h / day and the illumination strength of 1,600-2,000Ix for 25-35days; cutting tender branch tips of the Ilex dabieshanensis K Yao.et M.P.Deng and grafting the tender branch tips into a secondary culture medium for carrying out the secondary culture for 25-35days; and connecting aseptic seedlings of Ilex dabieshanensis K Yao.et M.P.Deng into the culture medium for rooting culture at the culture temperature of 20-26DEG C, the illuminaiton time of 10-16h / day and the illumination intensity of 1,600-2.000Ix. By using the method, the problem that the Ilex dabieshanensis K Yao.et M.P.Deng is quickly popularized can be effectively solved.

Description

technical field [0001] The invention relates to a Dabieshan holly 1# (Ilex dabieshanensis K Yao.et M.P.Deng.) tissue culture propagation method, belonging to the technical field of woody plant seedling propagation. Background technique [0002] Dabieshan holly (Ilex dabieshanensis K Yao.et M.P.Deng.) belongs to the genus Ilex of the family Aquifoliaceae. Evergreen small tree, about 5 meters high, the whole plant is glabrous, the bark is off-white, smooth; the branchlets are dark red, angular, leathery, ovate-oblong, ovate or elliptic, the apex is sharp and pointed, The base is nearly round, the edge is slightly reversed, with 4-8 pairs of spines, the upper part is dark green and shiny, the flowering period is March-April, the fruit period is October-November, the drupe is nearly spherical to oval, bright red when mature. Resistant to pruning; light-loving and semi-shady, cold-resistant and drought-tolerant; soil requirements are not high, and it grows luxuriantly in acidic ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 陆小清李乃伟李云龙郭忠仁
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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