Microorganism compound bactericide for controlling cucumber fusarium wilt and preparation method thereof
A cucumber fusarium wilt and compound bacterial agent technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, botany equipment and methods, etc., can solve the environmental suitability and effect stability limitations of preparations, and the control effect of cucumber wilt Limited and difficult to meet the requirements of the soil environment, etc., to achieve the effect of ensuring physiological activity and stability, ensuring universality, and ensuring compatibility
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Embodiment 1
[0027] A microbial composite bacterial agent for preventing and treating cucumber wilt comprises 10 parts by weight of Rhodopseudomonas palustris, 30 parts by weight of Bacillus subtilis, 15 parts by weight of Streptomyces, 20 parts by weight of Lactobacillus plantarum and 10 parts by weight of Saccharomyces cerevisiae.
[0028] Its preparation method is:
[0029] The single bacterial strain obtained by common method expansion and cultivation is mixed in the following proportions: 10 parts by weight of Rhodopseudomonas palustris, 30 parts by weight of Bacillus subtilis, 15 parts by weight of Streptomyces, 20 parts by weight of Lactobacillus plantarum and 10 parts by weight of Saccharomyces cerevisiae parts by weight;
[0030] The mixed bacterial solution was inoculated in the liquid medium after sterilization at 120°C for 30 minutes according to the weight ratio of 1:25. 0.4 parts by weight of sodium monohydrogen phosphate, 0.001 parts by weight of manganese sulfate, 0.001 pa...
Embodiment 2
[0041] A microbial compound bacterial agent for preventing and treating cucumber wilt comprises 15 parts by weight of Rhodopseudomonas palustris, 40 parts by weight of Bacillus subtilis, 18 parts by weight of Streptomyces, 23 parts by weight of Lactobacillus plantarum and 15 parts by weight of Saccharomyces cerevisiae.
[0042] Its preparation method is:
[0043] The single strains obtained by the expansion and cultivation of ordinary methods are mixed in the following proportions: 15 parts by weight of Rhodopseudomonas palustris, 40 parts by weight of Bacillus subtilis, 18 parts by weight of Streptomyces, 23 parts by weight of Lactobacillus plantarum and 15 parts by weight of Saccharomyces cerevisiae share;
[0044] Inoculate the mixed bacterial solution in the liquid medium after sterilization at 130°C for 45 minutes according to the weight ratio of 1:30. The liquid medium consists of 20 parts by weight of corn flour, 10 parts by weight of soybean meal powder, and 2 parts by...
Embodiment 3
[0046] A microbial composite bacterial agent for preventing and treating cucumber wilt comprises 12 parts by weight of Rhodopseudomonas palustris, 35 parts by weight of Bacillus subtilis, 16 parts by weight of Streptomyces, 22 parts by weight of Lactobacillus plantarum and 12 parts by weight of Saccharomyces cerevisiae.
[0047] The single strains obtained by the expansion and cultivation of ordinary methods are mixed in the following proportions: 12 parts by weight of Rhodopseudomonas palustris, 35 parts by weight of Bacillus subtilis, 16 parts by weight of Streptomyces, 22 parts by weight of Lactobacillus plantarum and 12 parts by weight of Saccharomyces cerevisiae share;
[0048] Inoculate the mixed bacterial solution in the liquid medium after sterilization at 125°C for 40 minutes according to the weight ratio of 1:26. The liquid medium consists of 10 parts by weight of corn flour, 5 parts by weight of soybean meal powder, and 1 0.6 parts by weight of sodium monohydrogen p...
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