Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method of molecularly imprinted polymer capable of enriching caffeic acid phenethyl ester

A technology of phenethyl caffeate and molecular imprinting, applied in the field of bioengineering, can solve the problems of limited natural sources and regional differences in content of phenethyl caffeate, and achieve the effects of low price, long service life and simple synthesis

Inactive Publication Date: 2011-04-13
NANKAI UNIV
View PDF1 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the natural sources of phenethyl caffeate are limited, and the reported natural products containing phenethyl caffeate mainly include propolis, poplar buds and honey, and the content has regional differences

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of molecularly imprinted polymer capable of enriching caffeic acid phenethyl ester
  • Preparation method of molecularly imprinted polymer capable of enriching caffeic acid phenethyl ester

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0023] A method for preparing molecularly imprinted polymers that can enrich phenethyl caffeate, the steps are as follows: 284 mg template molecule phenethyl caffeate and 420 μL functional monomer 4-vinylpyridine are dissolved in a mixture of 2 mL tetrahydrofuran and 3 mL isooctane In the solvent, mix well and stir for 2 hours; add 1882μL cross-linking agent ethylene glycol dimethacrylate, mix well and stir for 10min; add 30mg initiator azobisisobutyronitrile, mix well and stir for 10min; mix the above mixture Blow nitrogen to deoxidize for 4 minutes, seal it, place it in a constant temperature water bath and react at 60°C for 24 hours to obtain a block polymer; grind the obtained block polymer with a mortar, screen with a grading sieve, and reserve 60 mesh-100 mesh polymer; the above polymer is extracted with 250mL Soxhlet extract to remove unreacted substances, the extraction time is 48h, the Soxhlet extract is a mixture of methanol and acetic acid, and its volume ratio is 9...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a preparation method of a molecularly imprinted polymer capable of enriching caffeic acid phenethyl ester. The molecularly imprinted polymer capable of enriching the caffeic acid phenethyl ester can be prepared through uniformly mixing, nitrogen-blow deoxidization, constant temperature water bath reaction, soxhlet extraction and drying with the caffeic acid phenethyl ester as a template molecule, a mixed solvent of tetrahydrofuran and isooctane as a pore-forming agent, 4-vinyl pyridine as a functional monomer, ethylene glycol dimethacrylate as a crosslinking agent and azodiisobutyronitrile as an initiator. The invention has the advantages that the prepared molecularly imprinted polymer capable of enriching the caffeic acid phenethyl ester has the advantages of simple synthesis, low cost, strong specificity, long service life and the like, can be used for separating and extracting the caffeic acid phenethyl ester from a natural product, in particular to natural products with low caffeic acid phenethyl ester content, and the molecularly imprinted polymer reaches the purposes of enrichment and purification.

Description

technical field [0001] The invention belongs to the field of bioengineering, in particular to a method for preparing a molecularly imprinted polymer capable of enriching phenethyl caffeate. Background technique [0002] Caffeic acid phenethyl ester (CAPE), first found in propolis, is the main active ingredient in propolis. Because of its wide range of biological activities, such as anti-inflammation, immune regulation, anti-oxidation and specific inhibitory effect on tumor cells, it has broad application prospects in food and medicine. At the same time, caffeic acid phenethyl ester also exhibits strong inhibitory activity on HIV-1 integrase and low cytotoxicity. However, the natural sources of phenethyl caffeate are limited. Currently, the reported natural products containing phenethyl caffeate mainly include propolis, poplar buds and honey, and the content has regional differences. Therefore, further development of natural resources containing phenethyl caffeate has impor...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C08F222/14C08F226/06C08F2/44C08J9/28C08J9/26B01J20/26B01J20/30
Inventor 刘方李宁王常容曲丽媛吕鹏云赵蕊丽石玢
Owner NANKAI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com