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Ocean low-temperature dextranase, enzyme producing method and strain S6-2 produced therefrom

A kind of dextranase, low temperature technology, applied in the field of marine low temperature dextranase and enzyme production and production bacteria S6-2

Inactive Publication Date: 2011-03-30
HUAIHAI INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on the production of dextranase by marine microorganisms has not been reported at home and abroad

Method used

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  • Ocean low-temperature dextranase, enzyme producing method and strain S6-2 produced therefrom
  • Ocean low-temperature dextranase, enzyme producing method and strain S6-2 produced therefrom
  • Ocean low-temperature dextranase, enzyme producing method and strain S6-2 produced therefrom

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0084] Example 1. A Vibrio S6-2 ( Vibrio sp. S6-2) CGMCC N0.4011. The strain has the following characteristics: the strain is a Gram-negative rod-shaped bacterium without spores, and its size is about 1.31-1.58 μm × 0.53-0.79 μm; the colony characteristics on the solid medium containing dextran: the diameter of the colony is 2mm-5mm, round Shape, milky white, moist, transparent, add 95% ethanol after the colony grows, freeze for 3~4 hours, a transparent circle appears around the colony; the strain V-P test is negative, MR test is positive, cannot grow without sodium chloride, can liquefy gelatin, and has movement Ability; can use sucrose, fructose, but can not use lactose, galactose, mannose, xylitol, cellobiose, arabinose, melibiose, sorbitol. The growth characteristics of Vibrio S6-2 are: the growth temperature range of the strain is 4-37°C, the growth pH range is 6-11; the growth NaCl concentration range is 0.5%-10%; the optimum growth temperature of the strain is 25°C ;...

Embodiment 2

[0085] Example 2. A method for Vibrio S6-2 CGMCC No.4011 producing low-temperature dextranase as described in Example 1, the steps are as follows: Vibrio S6-2 ( vibrio sp. S6-2) strains were inoculated into 2216E medium, the rotation speed was 180r / min, the liquid volume was 20%, and the seed liquid was obtained after culturing for 12 hours; min, cultured at 25°C for 24h, centrifuged at 10000r / min for 5min, and the supernatant was prepared by ammonium sulfate, DEAE-sepharose Flat Flow and Sephacryl S-200 methods to prepare low-temperature dextranase.

Embodiment 3

[0086] Example 3. A low-temperature dextranase produced by the method described in Example 2, the low-temperature dextranase has the following characteristics: the properties of the low-temperature dextranase are: the optimum action temperature of the dextranase is 20°C, and it is incubated at 60°C for 2h Afterwards, the enzyme still has more than 50% activity; dextranase is stable in the range of pH6.0-10.0, Hg 2+ , Pb 2+ , Mn 2+ , Mg 2+ , Li 2+ 、Co 3+ 、Cu 2+ , Zn 2+ have varying degrees of inhibition on enzymes; Al 3+ The enzyme can be activated; SDS, urea, DTT and NBS can inhibit the enzyme.

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PUM

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Abstract

The invention discloses a vibriosp S6-2(Vibriosp S6-2) CGMCCN0.4011. The growth temperaute of the strain ranges from 4 to 37 DEG C, 25 DEG C is the most preferable; the growth pH ranges from 6 to 11, 7.0 is the most preferable; and the concentration of NaCl for growth is 0.5% to 10%, 4% is the most preferable. The invention further discloses a method for producing ocean low-temperature dextranase by vibriosp and a low-temperature dextranase product produced according to the method. The low-temperature dextranase plays an important role in the aspects of prevention and treatment of dental caries, sugar industry, production of pharmaceutical dextran and the like.

Description

technical field [0001] The present invention relates to a kind of microorganism, especially a kind of Vibrio S6-2 ( Vibrio sp. S6-2) CGMCC No. 4011 (preserved in CGMCC, General Microorganism Center of China Microbiological Culture Collection Management Committee on July 16, 2010, and the preservation number is CGMCC No. 4011); the present invention also relates to the production of marine Process for low temperature dextranase and products thereof. Background technique [0002] Dextran (a homoglycan of D-glucopyranose) is a polysaccharide with 95% α-1,6 sugar anhydride bonds. Dextranase (Dextranase, α-D-1,6-Glucan-6-D-Glucanohydrolase, EC3.2.1.11), also known as α-glucanase, is a specific cutting α-1 in dextran (Dextean), 6-glucose anhydrolase. Dextranase has very important application value, and plays an increasingly important role in the prevention and treatment of dental caries, the sugar industry and the production of medicinal dextran. Dextranase degrades polysacch...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N9/46C12R1/63
Inventor 王淑军吕明生房耀维焦豫良刘姝曹茜李瑛王东
Owner HUAIHAI INST OF TECH
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