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Rhesus monkey temporal lobe epilepsy kindling model and drug screening method thereof

A kind of ignition model, rhesus monkey technology, applied in the field of rhesus monkey temporal lobe epilepsy ignition model of rhesus lactone, can solve the problems of easy death, unsatisfactory, complicated operation, etc., to achieve good drug safety, Short epilepsy time and good reproducibility

Inactive Publication Date: 2012-09-19
WEST CHINA HOSPITAL SICHUAN UNIV +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

1997, 53: 239-258.) proposed that the drug-resistant epilepsy model should have the following characteristics: (1) the seizure type is similar to that of clinical drug-resistant epilepsy; (2) the model has paroxysmal abnormal discharges in the brain, And can evaluate drug efficacy from electrophysiological aspects; (3) AED cannot control its attack satisfactorily; (4) Model animals can survive for a long time
The only internationally recognized drug-resistant epilepsy model is the phenytoin-resistant amygdala electric ignition model in rats established by Loscher et al., but the disadvantages of this model are: complicated operation, difficult to raise animals, low ignition rate, easy death, and drug resistance. Narrow spectrum, mechanical damage to rat brain tissue
At present, there are few studies on primate epilepsy models, and there are no reports in China. Foreign countries are mainly limited to local injection of epilepsy agents such as alumina gel, bicuculline, etc., and electrical stimulation of the amygdala and cingulate gyrus. kindle model

Method used

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  • Rhesus monkey temporal lobe epilepsy kindling model and drug screening method thereof
  • Rhesus monkey temporal lobe epilepsy kindling model and drug screening method thereof
  • Rhesus monkey temporal lobe epilepsy kindling model and drug screening method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1 The establishment of temporal lobe epilepsy animal model of the present invention

[0034] 1 material

[0035] 1.1 Experimental animals

[0036] Five healthy male rhesus monkeys were purchased from Ping An Animal Breeding Center. They were 3 years old and weighed 7.5kg, 4.7kg, 5.1kg, and 4.2kg. Feed 2 times a day, supplemented with vegetables and fruits, drink water from automatic drinking fountains, the average indoor temperature is (21.1±13.6) ℃, relative humidity is 87.5±12.6, and there is a 3-week adaptation time before the experiment. System check and EEG examination to rule out abnormalities.

[0037] 1.2 Experimental drugs

[0038]Masangolactone injection: 63.5 kg of Masangolide was collected in Xichang, Sichuan in October, provided by Chengdu University of Traditional Chinese Medicine. Mix the ethanol and let stand overnight, recover ethanol under reduced pressure, concentrate, add NaCl to saturation, stand overnight, filter and retain the filtr...

Embodiment 2

[0087] Embodiment 2 The method for screening medicine of the present invention

[0088] a, adopt the rhesus monkey temporal lobe epilepsy ignition model prepared in Example 1;

[0089] b. Applying the drug to the model described in step a;

[0090] c. Observe the effect of the candidate drug on inhibiting the temporal lobe epilepsy in the model animal caused by the mosan lactone, and perform scoring to evaluate the potential drug for the treatment of the temporal lobe epilepsy. The evaluation method is to score whether there are significant differences in the decline in seizure frequency, seizure duration and seizure grade during the observation period.

[0091] The observation period is within 1 month. Observing significant differences in the frequency of seizures, duration of seizures and seizure grades indicates that the candidate drug is a drug for the treatment of temporal lobe epilepsy; the seizure grades are graded according to the racine grading method .

[0092] In...

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Abstract

The invention provides a rhesus monkey temporal lobe epilepsy kindling model. In the model, coriamyrtin with the subthreshold seizure dose of 0.5 ml / kg is applied in rhesus monkey, and the effect of 1ml of coriamyrtin injection is equivalent to that of 6.7g of raw material medicine Chinese coriaria leaf. The invention also provides a method for screening drugs for curing temporal lobe epilepsy. The method of the invention has the following advantages: the method is simple to establish and easy to operate and repeat, does not cause mechanical damage on the brain, has short seizure time, etc. The model of the invention resists the classical antiepileptic drug carbamazepine, thus the model can be used in the research on the pathogenesis of the temporal lobe epilepsy and the research on the development of new antiepileptic drugs.

Description

technical field [0001] The invention relates to an ignition model of rhesus monkey temporal lobe epilepsy, in particular, the use of the rhesus monkey temporal lobe epilepsy ignition model of the use of ethanolactone in the preparation of medicines. Background technique [0002] Epilepsy is one of the most common nervous system diseases. In my country, the incidence rate is 35 / 100,000·year, and the prevalence rate is 5‰, of which intractable epilepsy accounts for about 30%. Recurrent epileptic seizures not only cause physical and mental damage to patients, but also cause heavy psychological burdens on patients and their families due to long-term medication and social discrimination. Although new antiepileptic drugs have been used in clinical practice in recent years, according to multiple clinical trials, they have not brought significant improvement in the treatment of refractory epilepsy patients except for safety and small side effects, and only about 5% of refractory epi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K49/00A61K31/365A01K67/027
Inventor 周东李宏霞何俐王莉杨天华洪桢李劲梅辜蕊鄢波张勤
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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