Tremella spore fermentation material bioactive feed additive

A technology of tremella spores and biological activity, applied in the field of preparation of animal microecological preparations, can solve the problems of unseen patents and related literature reports

Inactive Publication Date: 2012-07-11
吴访其 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The tremella spore fermented product with biological activity function of the present invention is used as an animal feed additive and has not been reported in relevant patents and related literature so far.
[0007] In addition, tremella spores are a type of yeast cells, just like Saccharomyces cerevisiae and Candida utilis, so far there have been no patents and related literature reports on using them as feed microbial additives (ie feed microecological preparations)

Method used

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  • Tremella spore fermentation material bioactive feed additive
  • Tremella spore fermentation material bioactive feed additive
  • Tremella spore fermentation material bioactive feed additive

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] The separation collection and purification of embodiment 1 white fungus spore

[0040] The inventor adopts from artificially cultivated white fungus or separates white fungus spores from natural wild white fungus fruiting bodies in nature, and utilizes fermentation technology to produce white fungus fermented liquid on a large scale, and its method is as follows:

[0041] 1. Separation and collection of Tremella spores

[0042] Tremella spores are isolated and collected from artificially cultivated tremella fruiting bodies or natural wild white fungus fruiting bodies.

[0043] Two, the preparation of pure white fungus spores

[0044] Take a little Tremella fungus spores in 1-2ml sterile normal saline, spread it on a 9 cm petri dish with potato juice agar medium after appropriate dilution, and culture at 25°C±1°C until the naked eye can observe it on the petri dish When the small white spotted colony is reached, take a sterile sample, and when the yeast-like spore cell...

Embodiment 2

[0056] The preparation of embodiment 2 tremella spore fermented liquid

[0057] 1. Seed tank growth and cultivation:

[0058] Tremella spores that grow well are inoculated into the sterilized seed tanks of the above-mentioned C medium, and grown and cultured at 25°C±1°C for 7 days, which is Tremella spore culture solution.

[0059] 2. Fermentation tank growth and cultivation

[0060] A. Culture medium preparation is the same as the above-mentioned D medium preparation method.

[0061] B. Get the 3%-5% inoculation amount of the above-mentioned well-cultured white fungus spore culture solution, inoculate in the fermenter container, the ventilation flow rate is 1:1, the rotating speed is 150-200rpm, and cultivated at 25°C ± 1°C for 6-8 days. After sampling, Tremella spores were counted up to 3×10 8 -6×10 8 / ml, stop the fermentation growth, it is Tremella spore fermentation liquid.

Embodiment 3

[0062] Embodiment 3 Preparation of Tremella spore fermented product bioactive feed additive product

[0063] The above Tremella spores 3 × 10 8 -6×10 8 Mix 5kg of fermented liquid with 1kg of wheat bran per ml, dry in an oven at ≤65°C, pulverize the dried product, and sieve it to obtain the final dry powder product of Tremella spores fermented biologically active feed additive.

[0064] Composition of additive dry powder products

[0065] Additive dry powder, moisture≤10%

[0066] The number of Tremella fungus spores per gram of additive dry powder is 13×10 8 -26×10 8 / g

[0067] Additive dry powder Tremella spore fermented product accounts for 12±1%

[0068] Additive dry powder auxiliary material wheat bran accounts for 78±1%.

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Abstract

The invention discloses a method for preparing tremella spore fermentation liquor. The method comprises the following steps of: separating a wild or artificially cultured tremella seed solid to obtain a tremella spore; separating and purifying; vaccinating the tremella spore in a culture medium comprising 100g / L of fresh white potatoes, 0.4 percent of glucose, 0.3 percent of corn steep liquor, 1 percent of yeast selenium powder and 0.05 percent of phosphate and having natural pH value; growing and fermenting; and preparing to obtain the tremella spore fermentation liquor. The invention also discloses a tremella spore fermentation bioactive feed additive product which is prepared by mixing 5kg of the obtained tremella spore fermentation liquor with 1kg of wheat bran (in the proportion of 1:5) and drying at a temperature not higher than 65 DEG C, wherein each gram of additive dry powder contains 13*108-26*108, and tremella spore fermentation materials of the additive dry powder occupy 12+ / -1 percent.

Description

technical field [0001] The invention relates to the preparation of animal microecological preparations, in particular to the preparation of feed additives by fermentation of feed microorganisms. Background technique [0002] Tremella fuciformis Berk, also known as Tremella fuciformis Berk, is the fruiting body of the Basidiomycetes Tremella family, and has a long history of medicinal use. Tremella is a precious medicine that has nourishing and recuperating functions and can be used to treat deficiency syndromes. At present, in addition to artificially cultivating Tremella fruiting bodies for medicinal purposes, Tremella spores are also submerged in fermentation culture, and the tremella polysaccharide obtained after the fermentation broth is extracted and purified is used for medicinal purposes. Preliminary clinical trials have proved that tremella polysaccharides have certain curative effects on some respiratory diseases such as chronic bronchitis and pulmonary heart disea...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G1/04C05G1/00A23K1/16A23K10/12A23K10/30A23K20/10
Inventor 吴访其王康宁
Owner 吴访其
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