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Automatic refining apparatus, multi-well plate kit and method for extracting hexane from biological samples

A biological sample, multi-well plate technology, used in DNA preparation, instrumentation, recombinant DNA technology, etc., can solve the problems of slow solution, flow through small holes, restrictions, etc.

Inactive Publication Date: 2011-03-23
BIONEER
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the problem with using fine particles or filter membranes is that the solution flows very slowly through the small pores
However, they are limited in convenient and rapid processing of multiple specimens

Method used

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  • Automatic refining apparatus, multi-well plate kit and method for extracting hexane from biological samples
  • Automatic refining apparatus, multi-well plate kit and method for extracting hexane from biological samples
  • Automatic refining apparatus, multi-well plate kit and method for extracting hexane from biological samples

Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach 1

[0054] Embodiment 1 relates to an automatic purification apparatus of the present invention, that is, an apparatus for separating a target material from a plurality of biological samples by using magnetic particles to be reversibly coupled to the target material. figure 1 with figure 2 The main components of the device according to Embodiment 1 are schematically shown. image 3 The device according to Embodiment 1 with the housing partially removed is schematically shown. Figure 4 is a perspective view of a substrate of the device according to Embodiment 1. Figure 5 The use of the substrate of the device according to Embodiment 1 is shown. Image 6 show Figure 5 Loading of high temperature reaction tubes as shown in . Figure 7 It is shown that the substrate of the device according to Embodiment 1 is accommodated in the housing. Figure 8 It is a perspective view of the multi-well plate sample box according to Embodiment 1. Figure 9 Extraction of DNA from blood acco...

experiment example 1

[0091] Utilize the equipment of embodiment 1 to extract chromosomal DNA

[0092] 1) Manufacture of a sample box for extracting chromosomal DNA

[0093] To manufacture a sample cartridge for extracting chromosomal DNA, a large amount of the previously prepared reagent was added to well B through well E of the sample cartridge of a 96-well plate. The components of the reagents used for the extraction of chromosomal DNA are described below. In the unit well B of the 96-well plate sample box, a cell lysis solution (pH 4.0-7.0) including 1-8 M guanidine hydrochloride as a lysis buffer for lysing the cells of the biological sample was added , 10-100mM tris (hydroxymethyl) aminomethane hydrochloride, 10-500mM sodium chloride and 1-50% surfactant (Triton X-100, Tween-20, Tween-80, NP-40 Wait). In unit well C, add alcohol (isopropanol or ethanol) to improve the binding of chromosomal DNA to the magnetic particles. In cell well D, add the aqueous suspension of magnetic particles. I...

Embodiment approach 2

[0107] Embodiment 2 relates to another automatic purification device of the present invention, namely, a device for separating a target material from a plurality of biological samples by using magnetic particles to be reversibly coupled to the target material . Figures 11 to 13 The main components of the device according to embodiment 2 are schematically shown.

[0108] refer to Figures 11 to 13 , the magnetic field applying unit includes a first row of magnet mounting units 191, a first row of gears 191G, a first row of rotating shafts 191S, a second row of magnet mounting units 192, a magnet mounting unit motor 192M, a second row of gears 192G, and a second row of rotating shafts. Shaft 192S.

[0109] refer to Figure 11 with 12 , the second row of magnet mounting units 192 includes a second row of rotating arms 192-2 and a second row of plate mounting seats 192-3.

[0110] refer to Figure 11 with 12 , the second row of rotating arms 192-2 are radially connected an...

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Abstract

The present invention relates to an automatic refining apparatus for separating target materials from a plurality of biological sample solutions by using magnetic particles to which target materials are to be reversibly coupled, and to a multi-well plate kit for use in the automatic refining apparatus. Further, the present invention relates to a method for extracting hexane from biological samples by using the above-described automatic refining apparatus. The present invention can be used in the automatic separation of hexane, protein, and the like from biological samples.

Description

technical field [0001] The present invention relates to an automatic purification device for separating a target material from a plurality of biological samples by using magnetic particles, and a multi-well plate kit used in the automatic purification device, the The magnetic particles are to be reversibly coupled to the target material. [0002] In addition, the present invention relates to a method for extracting nucleic acid from a biological sample by using the above-mentioned automatic purification device. Background technique [0003] Various techniques have been developed for isolating nucleic acids, proteins, etc. from biological samples. Traditionally, precipitation, liquid phase extraction, electrophoresis, chromatography, etc. are commonly used. More recently, solid phase extraction has been developed for simpler handling. In solid phase extraction techniques, highly selective solid materials or solid particles to which highly selective ligands are attached are...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N35/00
CPCG01N35/1074C12N15/1013G01N35/0098
Inventor 金钟勋金钟甲李洋源朴翰浯
Owner BIONEER
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