Colloidal gold chromatography test paper for detecting glibenclamide and derivatives of glibenclamide quickly and preparation method thereof

Abstract

The invention discloses a colloidal gold chromatography test paper for detecting glibenclamide and derivatives of glibenclamide quickly, which consists of a substrate, a sample pad, a gold-marked combined pad, a covering film and an absorbent pad, wherein the gold-marked combined pad is polyester fibrofelt capable of absorbing the gold-marked antibody of the derivatives of glibenclamide; and a linear detection wire T coated with carrier protein solution for coupling the derivatives of glibenclamide and a linear reference wire C coated with goat anti-mouse IgG solution are arranged on the covering film, and the two wires are arranged parallelly. The method comprises the following steps: preparing artificial conjugated antigen; preparing monoclonal antibodies of the derivatives of glibenclamide; preparing solution of colloidal gold; and obtaining the colloidal gold marker of the monoclonal antibodies of the derivatives of glibenclamide and the gold-marked combined pad. The test paper has the advantage of high specificity, high sensitivity, simplicity, quickness, high timeliness, vivid result display and accuracy.

Description

technical field [0001] The invention relates to a colloidal gold chromatography test strip and a preparation method for rapidly detecting glibenclamide and its derivatives, belonging to the technical field of detection of biological immune methods. Background technique [0002] Glibenclamide is a sulfonylurea oral hypoglycemic drug, suitable for mild to moderate non-insulin-dependent diabetes mellitus whose curative effect is not satisfied with diet alone. Patients with pancreatic islet B cells have a certain function of secreting insulin and have no serious complications , for non-insulin-dependent (adult, obese) diabetic patients. In recent years, in order to make huge profits, some unscrupulous manufacturers have illegally added glibenclamide and other hypoglycemic western medicines such as glibenclamide, which are relatively cheap and quick to act, into traditional Chinese medicine or health care products for the treatment of diabetes without approval. It is a very comm...

AI Technical Summary

Problems solved by technology

However, in recent years, the means of illegal production by criminals have become increasingly concealed, and because the ingredients of traditional Chinese medicine are relatively complex, the detection is difficult, and sophisticated analytical instruments and professional technicians are required, resulting in a high cost of supervision and random inspection by the regulatory department, but the effect is not satisfactory.

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