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Biological functionalized gold nanorod molecular probe as well as preparation method and application thereof

A technology of biological functionalization and gold nanorods, which is applied in the application field of biological nanomaterials, can solve problems such as gold nanorod erosion and cell damage, and achieve good biocompatibility, simple surface functionalization process, and fluorescence stability Good results

Inactive Publication Date: 2011-03-02
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when two-photon imaging technology excited by near-infrared light is used to image gold nanorod-labeled cells, it is easy to cause gold nanorod ablation and cell damage.
There is no report on multicolor labeling of cells using the fluorescence properties of gold nanorods

Method used

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  • Biological functionalized gold nanorod molecular probe as well as preparation method and application thereof
  • Biological functionalized gold nanorod molecular probe as well as preparation method and application thereof
  • Biological functionalized gold nanorod molecular probe as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 Surface biofunctionalized gold nanorod molecular probe and its preparation method and application

[0032] (1) Preparation of gold nanorods with a certain aspect ratio:

[0033] Take 5.0mL 5.0×10 -4 mol / L of chloroauric acid was added to 5.0mL 0.20mol / LCTAB solution, and then 0.60mL 1.0×10 -2 mol / LNaBH 4 , stirred to obtain seed crystals; 3.0mL1.0×10 -3 mol / L chloroauric acid was added to 50μL 4.0×10 -3 mol / LAgNO 3 , 3.0mL 2.0×10 -1 mol / L CTAB solution, add 50μL 7.88×10 -2 mol / L ascorbic acid, and finally add 12 μL of seed crystals, and stir at constant temperature to obtain a gold nanorod stock solution. The aspect ratio of gold nanorods is about 4:1 ( figure 1 ).

[0034] (2) Preparation of replacing CTAB on gold nanorods with cystamine dihydrochloride:

[0035]Take 5.0 mL of gold nanorod stock solution, centrifuge for 10 min and redisperse in high-purity water, add 0.3 mL, 3.0×10 -2 mol / L cystamine dihydrochloride, ultrasonicated at 30°C for 2 ho...

Embodiment 2

[0041] Example 2 Surface biofunctionalized gold nanorod molecular probe and its preparation method and application

[0042] (1) Preparation of gold nanorods with a certain aspect ratio:

[0043] Take 5.0mL 5.0×10 -4 mol / L of chloroauric acid was added to 5.0mL 0.20mol / LCTAB solution, and then 0.60mL 1.0×10 -2 mol / LNaBH 4 , stirred to obtain seed crystals; 15.0mL 1.0×10 -3 mol / L chloroauric acid was added to 350μL 4.0×10 -3 mol / L AgNO 3 , 15.0mL 2.0×10 -1 mol / L CTAB solution, add 80μL 7.88×10 -2 mol / L ascorbic acid, and finally add 12 μL of seed crystals, and stir at constant temperature to obtain a gold nanorod stock solution.

[0044] (2) Preparation of replacing CTAB on gold nanorods with cystamine dihydrochloride:

[0045] Take 20.0 mL of gold nanorod stock solution, centrifuge for 30 min and redisperse in high-purity water, add 1.5 mL, 3.0×10 -2 mol / L cystamine dihydrochloride, ultrasonicated at 60°C for 5h, centrifuged for 30min, centrifuged to remove CTAB and ex...

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Abstract

The invention discloses a biological functionalized gold nanorod molecular probe as well as a preparation method and application thereof. The preparation method comprises: cystamine dihydrochloride substitutes for cetyltrimethyl ammonium bromide (CTAB) on the gold nanorod, and EDC / NHS is coupled with specific biomolecules to obtain the biological functionalized gold nanorod molecular probe. The biological functionalized gold nanorod molecular probe has the advantages of stable fluorescence performance, favorable biocompatibility and simple surface functionalization process, has the functions of near-infrared absorption and fluorescence emission, can label cells or tissues in a specific way, and can emit fluorescence with different colors by regulating different excitation wavelengths, thereby realizing the goal of carrying out multicolor labeling by using a single gold nanorod molecular probe. The biological functionalized gold nanorod molecular probe has strong drift resistance, and is a novel fluorescent probe which can replace the traditional fluorochrome.

Description

technical field [0001] The invention relates to the application field of bionano materials, in particular to a biofunctionalized gold nanorod molecular probe and its preparation method and application. Background technique [0002] With the development of fluorescence analysis and detection technology, fluorescent dyes have been widely used in gene recombination detection, flow cytometry, DNA hybridization test, immune detection, early diagnosis of tumor cells, etc. Traditional fluorescent probes are mainly organic fluorescent dyes, which have many disadvantages, such as easy photobleaching, poor fluorescence stability, narrow excitation spectrum, and wide emission spectrum; at the same time, different fluorescent dye molecules need multiple excitation wavelengths, and different colors of fluorescent molecules The spectra are easy to overlap each other, and it is cumbersome to use more than two kinds of fluorescent molecules for multi-color labeling; and the general fluoresc...

Claims

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Application Information

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IPC IPC(8): G01N33/533G01N21/64B82B1/00C09K11/58
Inventor 杨培慧姚述光刘媚蔡继业
Owner JINAN UNIVERSITY
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