Staphylococcus aureus enterotoxin gene PCR (Polymerase Chain Reaction) parting detection kit and method
A staphylococcus entero and detection kit technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve the problem of undetected Staphylococcus aureus enterotoxin, etc.
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[0047] Make PCR-based Genotyping Detection Kit for Staphylococcus aureus Enterotoxin according to the following formula:
[0048] (1) Amplification reaction solution components:
[0049] 10×Taq DNA Polymerase Buffer, 250mM dNTP, ddH 2 O: 10×Taq DNA Polymerase Buffer contains 200mM trishydroxymethionine methane-hydrochloride pH 8.4, 200mM potassium chloride, 100mM ammonium sulfate, 15mM magnesium sulfate and 1% Triton X-100.
[0050] (2) Typing reaction solution A: Contains 20uM each of SEA upstream and downstream primers:
[0051] SEA-F: 5′-CCGAGTCACGATCAATTTTTACAG-3′
[0052] SEA-R: 5'-GCGGCTTGTATATAAATATATATCA-3';
[0053] (3) Typing reaction solution B: Contains 20uM each of SEB upstream and downstream primers:
[0054] SEB-F: 5′-AATCTATAGATCAATTTCTATAC-3′
[0055] SEB-R: 5'-CTTTTTCTTTGTCGTAAGATA-3';
[0056] (4) Typing reaction solution C: Contains 20uM each of SEC upstream and downstream primers:
[0057] SEC-F: 5′-GTCTGTAGATAAATTTTTGGCA-3′
[0058] SEC-R: 5'-TCCA...
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