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Sphingosine monad DX-T3-03 strain and extracting method thereof

A sphingomonas and extraction method technology are applied in the field of sphingomonas DX-T3-03 strain and its extraction to achieve the effects of good degradability, environmental friendliness and low nutritional requirements

Inactive Publication Date: 2011-02-16
DONGHUA UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, phenol-containing industrial wastewater often contains high concentrations of heavy metal ions, and the presence of high concentrations of heavy metal ions will inhibit the biodegradation of phenol by microorganisms

Method used

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  • Sphingosine monad DX-T3-03 strain and extracting method thereof
  • Sphingosine monad DX-T3-03 strain and extracting method thereof
  • Sphingosine monad DX-T3-03 strain and extracting method thereof

Examples

Experimental program
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Embodiment 1

[0033] (1) Get soil from a mine tailings dam to make soil suspension and inoculate it in zinc-containing inorganic salt medium with phenol as the only carbon source by 5wt% insertion amount, wherein the inorganic salt medium is (NH 4 ) 2 SO 4 10.0g, KH 2 PO 4 7.5g, Na 2 HPO 4 10.0g, FeCl 3 0.0125g, MgSO 4 ·7H 2 O 0.015g, distilled water 1000mL, pH=6.5~7.0. Simultaneously add gradient concentrations of phenol and Zn 2+ solution, so that the medium contains both phenol and Zn 2+ And the concentrations of the two substances all increased sequentially, phenol 500, 600, 700, 800, 900, 1000mg / L, Zn 2+ The concentration is 0.5, 1.0, 1.5, 2.0, 2.5, 3.0, 3.5, 4.0mM / L, the interval between changing the concentration is 6-7 days, and the domestication culture is carried out at 30-35 °C and the shaking rate is 150r / min for 2 months;

[0034] (2) Take the sample after 2 months of acclimation, let it stand for 30 minutes, take 1mL of the upper layer water sample, and make 10 ...

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Abstract

The invention relates to a sphingosine monad DX-T3-03 strain and an extracting method thereof. The sphingosine monad DX-T3-03 strain has high resistance against zinc and phenol degradation capability, is separated and extracted from the soil of a tailing dam of a mine, achieves high tolerance to the zinc at 4 mM / L and high degradation rate of phenol at 88 percent, has low requirements on nutrition and important application potential in biologically treating double pollution of phenol-containing industrial waste water and heavy metals; in addition, the extracting method is simple, convenient, fast, low in cost and environmental-friendly.

Description

technical field [0001] The invention belongs to the field of sphingomonas bacterial strains, and in particular relates to a sphingomonas bacterial strain DX-T3-03 and an extraction method thereof. Background technique [0002] With the development of the mining industry and the application of heavy metals in electroplating, metallurgy, chemical and pharmaceutical industries, heavy metal pollution has become increasingly serious. Due to the characteristics of heavy metals in the soil, such as difficult to degrade, difficult to migrate, and long-term damage, soil heavy metal pollution has become one of the environmental problems that are widely concerned at home and abroad. Among them, the heavy metal zinc is not only an important nutrient element, but also a polluting element. Zinc was once known as the "flower of life" and is an important substance to maintain the normal growth and metabolism of the body. my country is a big zinc-producing country, and the largest consumpt...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/01
Inventor 柳建设王慧萍王志楼谢学辉付瑾张诚张萌张涛郑青凤
Owner DONGHUA UNIV
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