Method for extracting tea oil by microwave-assisted aqueous enzymatic method

A microwave-assisted, water-enzymatic technology, which is applied in the production of fat/fat and fat production, can solve the problems of difficult extraction of tea saponin, high acid value of oil and low oil yield, etc., and achieves easy extraction and utilization and nutritional value. High, low-equipment effects

Inactive Publication Date: 2012-11-07
NANCHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The production equipment of the water substitution method is simple, easy to operate, and low in cost; however, the oil yield is low, and the acid value of the proposed oil is high, and the water is relatively large, and water treatment and residue treatment, especially the extraction of tea saponin, are difficult

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The tea seeds are dehulled, dried and crushed. Weigh 4.00 g of crushed tea seeds, microwave for 60 s, add 12 ml of 0.05 mol / L sodium hydroxide salt solution, and bathe in 70°C water for 40 min. After cooling slightly, centrifuge and absorb the supernatant oil. Then use 18% hydrochloric acid solution to adjust the pH value to 4.5, add 0.5% compound enzyme (protease + cellulase), bathe in water at 40°C for 3 hours, and adjust the pH value to 8 with 10 mol / L sodium hydroxide solution ~9. Centrifuge and absorb the supernatant oil. Merge the camellia oil gained twice, vacuum dry, weigh. Finally, the yield of camellia oil was calculated to be 36.35%.

Embodiment 2

[0020] The tea seeds are dehulled, dried and crushed. Weigh 50.00 g of crushed tea seeds, microwave for 90 s, add 150 ml of 0.05 mol / L sodium hydroxide salt solution, and bathe in 80°C water for 30 min. After cooling slightly, centrifuge and absorb the supernatant oil. Then adjust the pH value of the degreased part to 4.0 with 20% hydrochloric acid solution, add 4% compound enzyme (protease + pectinase), bathe in water at 45°C for 2 hours, and adjust the pH value to 8 with 10 mol / L sodium hydroxide solution ~9. Centrifuge and absorb the supernatant oil. Merge the camellia oil gained twice, vacuum dry, weigh. Finally, the yield of camellia oil was calculated to be 37.82%.

Embodiment 3

[0022] The tea seeds are dehulled, dried and crushed. Weigh 100.00 g of crushed tea seeds, microwave for 80 s, add 300 ml of 0.05 mol / L sodium hydroxide salt solution, and bathe in 90°C water for 30 min. After cooling slightly, centrifuge and absorb the supernatant oil. Then adjust the pH to 4.5 with 20% hydrochloric acid solution, add 2% compound enzyme (protease + cellulase), bathe in water at 45°C for 4 hours, and adjust the pH to 8 with 10 mol / L sodium hydroxide solution ~9. Centrifuge and absorb the supernatant oil. Merge the camellia oil gained twice, vacuum dry, weigh. Finally, the yield of camellia oil was calculated to be 38.04%.

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Abstract

The invention discloses a method for extracting tea oil by a microwave-assisted aqueous enzymatic method, which is characterized by comprising the following steps of: (1) hulling tea seeds, drying the hulled tea seeds for 0.5 to 2 hours at 60 to 105 DEG C, and performing crushing; (2) weighing tea seed kernels obtained by the crushing, performing microwave treatment for 50 to 90 seconds, adding the treated tea seed kernels into 0.05 to 1 mol / L alkaline salt solution according to the weight / volume (w / v) of 1:3-6, performing water bath for 30 minutes at 90 DEG C, performing centrifugation and extracting upper-layer oil; (3) adjusting the pH value of a part of de-oiled mixed solution to be 4.0 to 4.5 by using acid solution, heating complex enzyme with the v / w of 0.5 to 4 percent, performing thermal treatment for 2 to 6 hours at 40 to 50 DEG C, adjusting the pH to be 8 to 9 by using alkaline solution, performing the centrifugation and extracting the upper-layer oil; and (4) combining the oil obtained in the steps (2) and (3) and performing vacuum drying. The method of the invention is simple, the extraction conditions are moderate, required equipment is relatively less and the production cost is low; over 36 percent of tea oil can be obtained at low temperature without any organic solvent; the obtained tea oil does not contain any trans fatty acid and foreign toxic residue, and has light color, a low acid value and a high nutritional value; tea saponin, proteins, carbohydrate and other nutrition in residues are easy to extract and utilize; and with a biochemical oxygen demand (BOD) value and a chemical oxygen demand value, wastewater produces less pollution and is easy to treat.

Description

technical field [0001] The invention belongs to the field of grain and oil processing, in particular to a method for extracting camellia oil from camellia seed kernels. technical background [0002] Camellia oil is light yellow in color, clear and transparent, with delicate smell, high quality and unique flavor; the unsaturated fatty acid in tea oil is as high as 90%, and it is rich in protein, vitamins A, B, D, E, tea polyphenols, squalene, etc. A variety of functional ingredients have obvious special effects on preventing cardiovascular sclerosis, lowering blood pressure, lowering blood fat, and preventing and fighting cancer. In addition, tea oil does not contain erucic acid, cholesterol, aflatoxin and other additives, and is a high-quality edible oil. [0003] The extraction of camellia oil includes pressing method, organic solvent extraction method, water substitution method and aqueous enzymatic method. At present, the commonly used methods in industrial production a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C11B1/00C11B1/04
Inventor 邓泽元杨辉刘蓉
Owner NANCHANG UNIV
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