Antimicrobial agent for gram-positive bacteria

A gram-positive, antibacterial agent technology, applied in the field of antibacterial agents for gram-positive bacteria, can solve the problem of unknown antibacterial effect of microparticle monomers, etc., and achieve the effect of broadening the treatment path

Inactive Publication Date: 2011-01-26
PUBLIC UNIV CORP YOKOHAMA CITY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in any research so far, the purpose is DDS and sustained release of the drug, and the antibacterial effect of the microparticle monomer is completely unknown

Method used

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  • Antimicrobial agent for gram-positive bacteria
  • Antimicrobial agent for gram-positive bacteria
  • Antimicrobial agent for gram-positive bacteria

Examples

Experimental program
Comparison scheme
Effect test

manufacture example 1

[0057] Production of Cyanoacrylate Polymer Particles

[0058] (dextran, dextran + glucose reaction system)

[0059]Cyanoacrylate polymer particles were produced using nBCA (Histoacryl (registered trademark), Braun Corporation, Melsungen, Germany) as a monomer. As the polymerization initiator-stabilizer, dextran alone or dextran+glucose was used. As the dextran, dextran (Dex70) having an average molecular weight of 70K was used.

[0060] Dissolve (1) 1 g of Dex70, or (2) 1 g of Dex70 and 5 g of glucose in 100 ml of hydrochloric acid. The concentration of hydrochloric acid is 0.05N, 0.01N, and 0.001N. While stirring each solution, 1.2 mL of nBCA (concentration in the reaction liquid: 1.2 v / v%) was added, and stirring was continued at room temperature for 2 hours (600 rpm) to carry out a polymerization reaction. 0.1N NaOH was added to the reaction liquid after polymerization to neutralize, and it stirred for 15 minutes after that. The reaction solution was filtered through a...

Embodiment 1

[0065] Antibacterial Activity of Cyanoacrylate Polymer Particles

[0066] (dextran, dextran + glucose reaction system)

[0067] The antibacterial activity (minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC)) of the particles produced in Production Example 1 against various bacterial strains was investigated. As a positive control, ampicillin (ABPC) was used. The assay method is in accordance with the microfluidic dilution method (NCCLS). That is, APBC uses 256 μg / ml as a stock solution (1-fold dilution), and particles use 6.4 mg / ml as a stock solution (1-fold dilution), and each prepares a 12-level dilution series ranging from 2-fold dilution to 2048-fold dilution, and evaluates the antibacterial activity . Add these dilution series to a 96-well plate, adding strains to each well to reach 10 5 CFU / ml, incubate at 35°C for 18 hours, if turbidity is observed visually, it is regarded as the strain has developed, and the lowest concentration wh...

manufacture example 2

[0078] Production of Cyanoacrylate Polymer Particles

[0079] (glucose, ribose, lactose, trehalose reaction system)

[0080] Cyanoacrylate polymer particles were produced using nBCA (Histoacryl (registered trademark), Braun Corporation, Melsungen, Germany) as a monomer. As the polymerization initiator-stabilizer, glucose, ribose, lactose, trehalose are used.

[0081] Except using any one of 5 g of the above-mentioned monosaccharides and disaccharides as sugars, the same operation as in Production Example 1 was carried out to produce polycyanoacrylate particles (0.05NHCl-Glucose, 0.05NHCl-Ribose, 0.05NHCl-Ribose, 0.05NHCl- Lactose, 0.05NHCl-Trehalose, 0.01NHCl-Glucose, 0.01NHCl-Ribose, 0.01NHCl-Lactose, 0.01NHCl-Trehalose, 0.001NHCl-Glucose).

[0082] The obtained particles were subjected to He·Ne laser light scattering analysis using a commercially available measuring device (mentioned above), and the average particle diameter and ζ-potential were measured. The results are ...

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Abstract

Disclosed is a novel antimicrobial agent for Gram-positive bacteria extending beyond the mechanism of bacterial drug resistance. The antimicrobial agent contains, as active ingredients, particles which adhere to a Gram-positive bacterial cell wall and do not adhere to a mammalian cell membrane, have a particle diameter of 5 [mu]m or less, and practically do not contain an antimicrobially active component against Gram-positive bacteria. According to the antimicrobial agent of the invention, antimicrobial effect also on Gram-positive bacteria showing multidrug resistance typified by MRSA and VRE can be achieved. Further, the emergence of new multidrug resistant bacteria which becomes a major problem in the use of an antibiotic can be avoided.

Description

technical field [0001] The present invention relates to an antibacterial agent for Gram-positive bacteria comprising, as an active ingredient, particles substantially free of an antibacterial active ingredient against Gram-positive bacteria. Background technique [0002] In recent years, many antibiotics have been continuously developed, which makes the illusion that bacterial infections have been overcome. However, bacteria have mastered the defense mechanism against various antibiotics and survived one by one, and cause nosocomial infections as pathogenic bacteria. major state of affairs. Methicillin-resistant Staphylococcus aureus (MRSA), vancomycin-resistant Enterococcus (VRE), vancomycin-resistant Staphylococcus aureus (VRSA), and multidrug-resistant Pseudomonas aeruginosa (MDRP), etc. Bacteria are resistant to almost all existing antibiotics, so the whole world is looking forward to finding countermeasures as soon as possible. [0003] The mechanism of antibiotic res...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/785A61K9/16A61P31/04
CPCA61K31/785A61K9/5138A61K9/14A61K9/5192A61P31/04Y10T428/2982
Inventor 城武升一
Owner PUBLIC UNIV CORP YOKOHAMA CITY UNIV
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