Light chain and heavy chain variable regions of anti-SEB (Staphylococcal Enterotoxin B) monoclonal antibody FMU-SEB-No.1 with high neutralizing activity
A technology of monoclonal antibody, fmu-seb-no.1, which is applied in the field of biomedicine to achieve strong neutralizing ability and high affinity
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[0030] The present invention immunizes Balb / c mice with natural SEB, prepares a group of mouse anti-SEB monoclonal antibodies, screens out the FMU-SEB-No.1 mAb hybridoma cell line that can stably secrete high-affinity anti-SEB, and prepares Obtain high-affinity anti-SEB mAb from ascites; and confirm the uniqueness of the gene sequence and corresponding protein sequence and its CDR sequence; provide support for anti-SEB chimeric or humanized genetic engineering antibodies. The present invention will be described in detail below in conjunction with the preparation method of specific monoclonal antibodies, antibody activity detection, and sequence detection and uniqueness determination, which is an explanation rather than a limitation of the present invention. The present invention is specifically implemented according to the following steps:
[0031] 1. Preparation of mouse anti-SEB high affinity antibody FMU-SEB-No.1 mAb
[0032] 1.1 Preparation and purification of monoclonal ...
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