Kit for detecting free thyroxin by using magnetic particles as solid-phase carriers and preparation method thereof

A technology of free thyroxine and magnetic particles, which is applied in the field of clinical immunoassay, can solve the problems of marker reduction and measurement error, and achieve the effect of simple magnetic separation

Active Publication Date: 2011-01-19
AUTOBIO DIAGNOSTICS CO LTD
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Problems solved by technology

[0005] Thyroxine is a small molecular substance, and immunoassay is determined by competition method. When free thyroxine is measured, the enzyme-labeled antigen wil

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Embodiment Construction

[0026] The kit for detecting free thyroxine using magnetic microparticles as a solid-phase carrier of the present invention comprises a suspension of magnetic microparticles coupled with derivatives of thyroxine analogues, and the magnetic microparticles are coupled with triiodothyronine Amino acid-gelatin complex; free thyroxine series calibrator prepared by adding dethyroxine serum or analysis buffer as the matrix and adding pure thyroxine; horseradish peroxidase-labeled anti-thyroxine antibody solution The anti-thyroxine antibody is a mouse anti-thyroxine monoclonal antibody; the luminescent substrate A liquid composed of 0.15mM luminol, 0.59mM hydroxycoumarin, 0.35mM gallic acid, and 0.2M Tris-Hcl buffer solution of pH 9.4 and Amino acid oxidase 0.85mM, Tween-200.8% v / v, diethylenetriaminepentaacetic acid 0.5mM, vitamin C 0.12mM, pH 6.5 acetic acid-acetate buffer 0.2M luminescent substrate B solution; and Concentrated wash solution consisting of 0.1M phosphate buffer, 1% T...

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Abstract

The invention discloses a kit for detecting free thyroxin by using magnetic particles as solid-phase carriers. The kit comprises magnetic particle suspension coupled with triiodothyronine-gelatin compound, free thyroxin series calibrator, horseradish peroxidase labeled antithyroidin antibody solution, luminous substrate liquid A and liquid B and concentration washing liquid. The invention also discloses a preparation method for the kit. The kit has the advantages that: because a magnetic particle chemical luminescent method is combined with a magnetic particle solid-phase coupling technique, the magnetic particles are suspended in the liquid in the absence of magnetic field, and the antigen antibody reaction is similar to homogeneous reaction; and the magnetic particles are convenient to separate under the action of an external magnetic field and quick to wash, and can improve the detection precision and stability when used as immunological carriers. The kit has the advantages of simple composition, convenient operation, stable and reliable result; and compared with the commercial elisa kits and plate-type chemical luminescent kits, the kit has the advantage that the reaction timeis only 1/8 to 1/4 of the time of the kits.

Description

technical field [0001] The invention relates to clinical immunoassay technology, in particular to a kit for detecting free thyroxine using magnetic microparticles as a solid phase carrier, and also relates to a preparation method of the kit. Background technique [0002] Thyroxine (T4) is a thyroid hormone synthesized and secreted by thyroid follicular epithelial cells. Its structure consists of a tyrosine residue and a phenolic ring. an iodine atom. The average daily T4 secretion of normal people is 90±9μg, which is about 1% of the storage capacity in the thyroid gland. After entering the blood circulation, about 99.96% of T4 binds to binding proteins, of which about 60% binds to TBG, 30% binds to TBPA, and the rest binds to albumin. There is a reversible equilibrium between bound T4 and free T4. Thyroxine in the bound state cannot exert its physiological functions, but only free T4 can enter the target cells to bind with T4 receptors and exert its physiological function...

Claims

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Application Information

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IPC IPC(8): G01N33/78G01N33/577G01N33/543G01N33/535
Inventor 靳增明陈晓玲付光宇渠海马建军项立红吴学炜苗拥军
Owner AUTOBIO DIAGNOSTICS CO LTD
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