Dot immuno-gold filtration assay (DIGFA) quick detection card for leather hydrolyzate in milk and preparation method thereof

Abstract

The invention relates to a dot immuno-gold filtration assay (DIGFA) quick detection card for leather hydrolyzate in milk and a preparation method thereof. The detection card is composed of a sample layer, a colloidal gold labeled layer, a detection layer, a water absorption layer, a plastic lining plate and a plastic cover plate. The preparation method comprises the following steps: preparing a colloidal gold labeled leather hydrolyzed protein solution and a colloidal gold labeled layer; preparing a leather hydrolyzed protein multi-clone antibody solution and detection lines on the detection layer; and assembling the detection card. The detection card has the advantages of simple operation and accurate detection result, can be interpreted by naked eyes, does not need complex operation, special equipment and the like, is suitable for on-site casual detection of leather hydrolyzate in milk for milk product enterprises, and inspection and sanitary departments, and has wide application prospects.

Description

technical field [0001] The invention relates to food safety detection, in particular to a gold label rapid detection card for leather hydrolyzate in milk and a preparation method thereof. Background technique [0002] Milk and dairy products are popular foods, and unscrupulous operators often adulterate milk in order to make huge profits, which seriously endangers the legitimate rights and interests of consumers. Key factor. Common milk adulteration methods include: adding water to increase weight, adding salt, nitrate and nitrite to increase specific gravity, mixing antibiotics, alkaline substances and formaldehyde to delay spoilage, adding fat powder to increase fat content; adding urea, Nitrogen-containing substances such as melamine. For this reason, governments at all levels have intensified their rectification efforts, and unscrupulous traders began to add a new type of adulterant—leather hydrolyzate—in order to evade the crackdown. [0003] Leather hydrolyzate is a...

AI Technical Summary

Problems solved by technology

The disadvantages are: 1. The reaction time is long, and the acid hydrolysis of the sample only takes 16-24 hours

2. The reagents are highly corrosive, and corrosive substances such as hydrochloric acid, sodium hydroxide, and phenol are used in large quantities, requiring staff to have specialized experimental skills

3. The requirements for instruments are high, and special instruments such as spectrophotometers, constant temperature water baths and heaters are required

But there are also obvious disadvantages: the casein precipitant mercury nitrate used is a heavy metal salt, which is highly toxic; and the results of this method are not easy to interpret accurately, and are easily interfered by milk acidity and foreign substances, resulting in false positives

This method has many advantages such as accurate test results, high sensitivity, good stability, and simple sample processing, but it requires expensive ion chromatographs and is difficult to popularize.

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