Method for rapidly propagating plantlets by utilizing bletilla striata seeds

Abstract

The invention relates to a method for rapidly propagating plantlets by utilizing bletilla striata seeds. The method comprises the following steps of: soaking and sterilizing bletilla striata seeds by adopting an iodophor solution; adopting an improved culture medium and reasonably adjusting the culture temperature in an intelligent climate box at a stage of inducing cluster buds; implanting seedlings which are cultured through lighting for 35-50 days into a stainless steel tray or a porcelain tray containing a substrate; hardening the seedlings indoors at 20-26 DEG C; and planting the bletilla striata seedlings in a field after the bletilla striata seedlings grow for 3-4 months. The invention shortens the seedling formation period, improves the survival rate of the bletilla striata seedlings in the field and avoids injuring human bodies by the prior art which sterilizes the bletilla striata seeds with a mercury chloride solution.

Description

technical field [0001] The invention belongs to the technical field of planting medicinal material bletilla striata in traditional Chinese medicine agriculture, in particular to a method for rapidly propagating seedlings by using bletilla striata seeds. Background technique [0002] Bletilla striata seeds, which are generally used for artificial cultivation, are used as tissue culture test materials for sterile seedling cultivation. Rinse the Bletilla striata capsules with drinking water for 25-35 minutes, then soak them in 70% alcohol for 3-4 minutes in an ultra-clean workbench, then soak them in 0.15% mercuric chloride solution for 10-15 minutes, and then use Rinse with sterile water 2-4 times, and dry the surface with sterile filter paper for later use. The culture conditions generally use KC medium + 200g / L fresh potato juice to induce the growth of clustered buds, and then insert the differentiated small buds into KC+6+BA2.5+NAA1.25mg / L medium to proliferate, so that ...

AI Technical Summary

Problems solved by technology

Then put the rootless seedlings into the culture medium of 1 / 2MS+6-BA0.5mg / L+NAA1.5mg / L+activated carbon 0.55%, the culture temperature is (25±2)℃, and the light time is 8-10 hours / day , the light intensity is 1500-2000LX, and 0.6% agar, 2% sucrose, and pH 5.5 are added to the culture medium, but they all stay in the laboratory and have not entered field production due to the low survival rate in the seedling hardening process. No bletilla striata has been seen so far A report on the application of rapid propagation technology in field production

And in the process of soaking Bletilla striata seeds with mercuric chloride, it is easy to cause harm to the human body