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Method for culturing edible and medicinal fungal liquid seeds

A technology for edible and medicinal fungi and liquid seeds, which is applied in the field of fermentation engineering, can solve the problems such as the lack of simultaneous inspection of production technology and the lack of innovation in cultivation methods, and achieve the effect of strong vitality

Inactive Publication Date: 2011-01-12
HARBIN INST OF TECH AT WEIHAI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the past, in the production of liquid seeds, the production of mycelium or the number of mycelium balls was mostly used as a single investigation object, and the production technology of both was not investigated at the same time; the selection of the medium formula was also selected from a few formulas, and did not pass mathematics. Optimize the model to obtain the best medium formula; there is also a lack of innovation in the culture method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] A method for cultivating edible and medicinal fungus liquid seeds, the culture medium formula (g / liter) used is glucose 15, potato 100, yeast extract 15, beef extract 12.5, first from the preserved strains, aseptically inoculated to On the plate solid medium, culture at a constant temperature of 20°C for 8 days; then use a 10mm diameter puncher to punch holes from the edge of the plate where the strains grow vigorously, take 10 strains, and inoculate them in 100mL liquid medium In a 250mL Erlenmeyer flask; static pre-cultivation at 20°C for 1 day; finally transferred to a shaker, and cultured in a constant temperature shaker at 20°C and 120 rpm for 10 days to obtain excellent liquid seeds. In the liquid seeds produced by the present invention, the output of mycelia is 7.4 grams per liter, and the number of mycelia balls is 122.8 per milliliter, which is 2.8 times and 7.8 times higher than that of the control culture method, and the strains are in logarithmic vigorous gro...

Embodiment 2

[0019] A method for cultivating edible and medicinal fungus liquid seeds, the culture medium formula (grams per liter) used is glucose 25, potato 150, yeast extract 15, beef extract 15, first from the preserved strains, aseptically inoculated to Cultivate at a constant temperature of 30°C for 5 days on a plate solid medium; then use a 10 mm diameter puncher to punch holes from the edge of the plate where the strains grow vigorously, take 10 strains, and inoculate them in 100mL liquid medium In a 250mL Erlenmeyer flask; pre-cultivate statically at 30°C for 1 day; finally transfer to a shaker, and cultivate in a constant temperature shaker at 30°C and 120 rpm for 8 days to obtain excellent liquid seeds. In the liquid seeds produced by the present invention, the output of mycelia is 8.3 grams per liter, and the number of mycelia balls is 192.5 per milliliter, which are respectively 3.2 times and 12.2 times higher than that of the control culture method, and the strains are in loga...

Embodiment 3

[0021] A method for cultivating edible and medicinal fungus liquid seeds, the culture medium formula (g / liter) used is glucose 35, potato 200, yeast extract 17.5, beef extract 17.5, first from the preserved strains, aseptically inoculated to On the plate solid medium, culture at a constant temperature of 25°C for 8 days; then punch a hole with a diameter of 10 mm from the edge of the above-mentioned plate where the bacteria grow vigorously, take 10 pieces of bacteria, and inoculate in 100mL liquid medium In a 250mL Erlenmeyer flask; static pre-cultivation at 25°C for 2 days; finally transferred to a shaker, and cultured in a constant temperature shaker at 25°C and 120 rpm for 9 days to obtain excellent liquid seeds. In the liquid seeds produced by the present invention, the output of mycelia is 8.8 grams per liter, and the number of mycelia balls is 206.3 per milliliter, which is 3.4 times and 13.1 times higher than that of the control culture method, and the strains are in log...

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Abstract

The invention relates to a method for culturing edible and medicinal fungal liquid seeds. The method comprises the following steps of: first, inoculating 10 flat plate strains with diameter of 10mm into a 250mL conical flask which holds 100mL of liquid culture medium with specific formulation; pre-culturing the strains still at the temperature of between 20 and 30 DEG C for 1 to 2 days; and continuously culturing the strains in a constant temperature shaking table at the temperature of between 20 and 30 DEG C and at the rate of 120r / m for 8 to 10 days so as to culture the high-quality liquid seeds. Both number of mycelium pellets and biomass of mycelium of the cultured liquid seeds are obviously increased by 7 to 14 times and 2 to 4 times compared with those of a contrast formula culture method respectively; and the seeds are in a logarithmic phase, have strong vitality and are high-quality liquid seeds. The method can be used for culturing various edible and medicinal fugal conical flask liquid seeds and producing liquid seeds in an automatic fermentation tank. The cultured liquid seeds are used for both liquid fermentation and solid fermentation and also used in cultivation of mushrooms.

Description

Technical field: [0001] The invention relates to the field of fermentation engineering, in particular to a method for cultivating liquid seeds of edible and medicinal fungi. Background technique: [0002] Liquid seeds are the basis of liquid fermentation of medicinal fungi, and are one of the key factors that determine the success or failure of liquid fermentation and the effect. Excellent seeds can not only significantly increase the production of mycelium and polysaccharides in the liquid fermentation process, but also shorten the fermentation process. time and improve the utilization rate of fermentation equipment; liquid seeds are also excellent seeds in solid fermentation of medicinal fungi and mushroom cultivation, which can significantly increase the speed of germination, shorten the germination period, advance mushroom harvest time and increase mushroom yield. [0003] In the past, in the production of liquid seeds, the production of mycelium or the number of myceliu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04C05G1/00
Inventor 闫培生朱国振高秀君董静
Owner HARBIN INST OF TECH AT WEIHAI
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