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Enzyme bioelectrochemical sensing chip and preparation and using methods thereof

A bioelectrochemical and sensor chip technology, applied in the fields of material electrochemical variables, gaseous chemical plating, circuits, etc., can solve problems such as the inability to use biochips, and achieve the effect of reasonable design, improved reliability and measurement accuracy

Inactive Publication Date: 2010-12-29
江苏益凯医疗器械有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method cannot be used for biochips in which the working and reference electrodes are arranged on the same plane, and requires the development and use of electronic devices with very complex data processing and algorithms

Method used

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  • Enzyme bioelectrochemical sensing chip and preparation and using methods thereof
  • Enzyme bioelectrochemical sensing chip and preparation and using methods thereof
  • Enzyme bioelectrochemical sensing chip and preparation and using methods thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0080] Preparation and testing of gold electrode biochip with sample injection on one side of the embodiment

[0081] In this embodiment, the enzyme bioelectrochemical sensor chip (hereinafter referred to as the biochip) adopts a single-sided gold-plated polyester sheet. First, a main electrode 102 with two branch electrodes is formed on the same plane by electric ablation. and an auxiliary electrode 103. The rectangular auxiliary electrodes 103 are arranged in parallel between two rectangular branch electrodes of the main electrode 102 . The two branch electrodes of the main electrode 102 have the same width of 0.6 mm, and the width of the auxiliary electrode 103 is 1.2 mm. The spacing between electrodes is 0.05 micron. Then, stick one side of an insulating double-sided adhesive tape about 0.13 mm thick with an opening about 0.8 mm wide on the surface of the polyester sheet with electrodes. The rectangular opening of the double-sided adhesive is vertically superimposed on ...

Embodiment 2

[0090] Preparation and testing of two-terminal sample-filled gold electrode biochips

[0091] The biochip of this embodiment adopts the same single-sided gold-plated polyester sheet as in Embodiment 1, and a main electrode 102 with two branch electrodes and an auxiliary electrode 103 are formed on the same plane by electroablation. The rectangular auxiliary electrodes 103 are arranged in parallel between two rectangular branch electrodes of the main electrode 102 . The arrangement direction and size of the two electrodes on the polyester sheet are different from those in Example 1. please see Image 6 , which shows the structure of the biochip of this embodiment, the spatial positions and interrelationships of each part. The dimensions in the figure are for illustrative purposes only and are not to scale.

[0092] The two branch electrodes of the main electrode 102 have the same width of 1.0 mm, and the width of the auxiliary electrode 103 is 1.0 mm. The spacing between th...

Embodiment 3

[0097] Preparation and testing of the three-terminal sample-filled graphite electrode biochip of the embodiment

[0098] The two electrodes of the biochip in this embodiment are attached to the surface of the polyester sheet with conductive carbon ink by screen printing, and then dried to form a conductive graphite layer with a thickness of about 10 microns. The rectangular auxiliary electrodes 103 are arranged in parallel between two rectangular branch electrodes of the main electrode 102 . The two branch electrodes of the main electrode 102 have the same width of 0.8 mm, and the width of the auxiliary electrode 103 is 1.6 mm. The spacing between the electrodes is 0.2 microns. Then, use insulating ink to form an insulating layer with a thickness of about 5 microns and an opening of 4.0 mm (length) x 0.7 mm (width) on the surface of the polyester sheet with two graphite electrodes by screen printing and drying. . The rectangular opening of the insulating layer is vertically...

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Abstract

The invention discloses an enzyme bioelectrochemical sensing chip and preparation and using methods thereof, and belongs to the technical field of analysis and detection. The enzyme bioelectrochemical sensing chip comprises an insulating substrate made of a conductivity insulating material, a main electrode attached to the insulating substrate, an auxiliary electrode attached to the insulating substrate and corresponding to the main electrode, a conducting circuit used for electrically connecting the main electrode, the auxiliary electrode and an electronic device, provided with a contact and attached to the insulating substrate, a reagent layer fully or partially covering the surfaces of the main electrode and the auxiliary electrode, an interlayer provided with an opening, made of a conductivity insulating material and covering the main electrode and the auxiliary electrode, and a surface covering layer made of the same conductivity insulating material and covering the interlayer. The enzyme bioelectrochemical sensing chip has the advantages of reasonable design, and effective improvement of use reliability and measurement accuracy of a biochip and products thereof.

Description

technical field [0001] The invention relates to an enzyme bioelectrochemical sensor chip for identifying and quantitatively detecting a specific solute in a liquid, and also relates to a preparation and use method of the bioelectrochemical sensor chip. More specifically, the present invention relates to a method for measuring enzyme-catalyzed redox reaction products by an electrochemical method, thereby realizing the identification and identification of solute components in liquid samples such as blood, serum and interstitials fluid. A biological chip for quantitative detection, and at the same time, the invention also relates to a method for preparing and using the enzyme bioelectrochemical chip. It belongs to the field of analysis and detection technology, which includes but not limited to medical care, environmental protection, food production and safety, etc. Background technique [0002] Enzymatic bioelectrochemical sensor (English name is enzymatic electrochemical bio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26B81B3/00B81C1/00
Inventor 刘丹
Owner 江苏益凯医疗器械有限公司
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