Method for identifying Nigella glandulifera Freyn medicinal material
A technology of Nigella cumulus and medicinal materials, which is applied in the field of identification of traditional Chinese medicinal materials, and achieves the effects of high precision, simple method, good stability and repeatability
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[0026] In order to better understand the present invention, the present invention will be further described below in conjunction with the examples, but the present invention is not limited thereto.
[0027] Embodiment of the present invention: identification method of the medicinal material of Nigella sativa.
[0028] 1 Instruments and reagents.
[0029] 1.1 Instruments.
[0030] Shimadzu LC-10ADvp high performance liquid chromatograph, SPD-M10Avp detector.
[0031] 1.2 Test drug.
[0032] A total of 10 batches of Nigella officinalis medicinal materials were collected from Xinjiang Hotan, Hotan Yutian, Kashgar, Kashgar Yuepu Lake, Qitai, Turpan, Yili, Shanshan and other places; the nigella glycoside reference substance was self-made, with a purity of more than 98 %; Acetonitrile is chromatographically pure, and other reagents are analytically pure.
[0033] 2 Methods and results.
[0034] 2.1 Determination of chromatographic conditions.
[0035] 2.2.1...
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