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Composition for human and/or animal nutrition, uses thereof and yeasts

A technology of yeast and composition, applied in the new field of yeast, can solve the problems of no beneficial effect or the same effect, different effects, etc., and achieve the reduction of intestinal bacteria, reduction of intestinal bacteria, and the best gastrointestinal effect. optimized effect

Active Publication Date: 2010-12-15
LESAFFRE & CIE +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But there are many strains of Saccharomyces cerevisiae and they have no beneficial effect or the same effect
[0010] Furthermore, depending on the strain used and the form of yeast applied, the effects described can also be very different

Method used

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  • Composition for human and/or animal nutrition, uses thereof and yeasts
  • Composition for human and/or animal nutrition, uses thereof and yeasts
  • Composition for human and/or animal nutrition, uses thereof and yeasts

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0106] Example 1: ScPro1 and / or SCB1 yeast in the artificial digestive environment of simulated human intestine survival in

[0107] Fate of ScPro1 and / or SCB1 yeasts in gastrointestinal transit

[0108] In vivo detection and study of ScPro1 and / or SCB1 yeasts in an artificial digestive environment simulating human digestion, in particular by studying the survival of the tested live yeasts during gastrointestinal transit.

[0109] Two samples of ScPro1 in the form of active dry yeast and two samples of SCB1 in the form of active dry yeast were tested.

[0110] The two samples were distinguished by the time they were kept in vacuum at room temperature: less than 6 months aged or 2 years aged.

[0111] ·Experimental conditions:

[0112] Digestion is carried out in the system known as T1M1 (stomach + small intestine) according to experimental conditions determined from data in the literature and remanufactured Digestion of liquid food (water) in healthy adults with an emp...

Embodiment 2

[0148] Example 2: Survival of ScPro1 yeast in an artificial digestive environment simulating the human intestine

[0149] Survival of ScPro1 yeasts during colonic fermentation and their impact on intestinal flora study

[0150] The active dry form of ScPro1 yeast was tested and studied in vitro in an artificial digestive environment simulating human digestion, and specifically by studying the fate and environmental effects of live yeast tested in colonic fermentation.

[0151] Colonic fermentation involves sequential replenishment of the medium to maintain continuous fermentation of the flora. Said medium mainly comprises complex carbohydrates, indigestible in the upper part of the digestive tract (starch, pectin, cellulose, etc.), more or less hydrolyzed protein compounds and mucins.

[0152] Colon medium was also removed from the fermenters in a sequential fashion. The medium is covered by a dialysis system, allowing continuous removal of soluble fermentation product...

Embodiment 3

[0181] Embodiment 3: Effect of ScPro1, SCB1 yeast on the induction of the production of cytokine Research

[0182] The effect of live ScPro1 and SCB1 yeasts on the induction of cytokine production on human peripheral blood mononuclear cells (PBMCs) was studied.

[0183] ScPro1 and SCB1 yeasts were tested for their ability to induce IL-10, ILK-12, TNFα, TNFδ cytokine production in human PBMCs in their flash dry and live dry forms.

[0184] Preparation of human peripheral blood mononuclear cells

[0185] Fresh human blood obtained from healthy experimenters at a blood transfusion center was diluted twice with PBS-Ca (GIBCO) and purified with a Ficoll density gradient (GIBCO). Peripheral blood mononuclear cells (PBMC) formed a circular layer in serum after centrifugation at 400 x g for 30 minutes at 20°C. Aspirate PBMC carefully, suspend in PBS-Ca in a final volume of 50ml, wash with the same buffer solution three times, and centrifuge at 350x g for 10 minutes at 20°C. P...

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PUM

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Abstract

The invention relates to novel yeast strains, to the yeasts resulting from these strains, to a composition containing at least one Saccharomyces cerevisiae yeast and / or derivatives of a yeast having a particular interest as a food additive and / or probiotic and / or functional food and / or neutraceutic and / or functional ingredient and / or cosmeceutical and / or pharmaceutical active agent. The inventionalso relates to the use of the same in human and / or animal nutrition, or for the treatment or prevention of inflammatory diseases.

Description

technical field [0001] The present invention relates to the field of human and / or animal nutrition and health. [0002] The present invention more particularly relates to novel yeast strains and novel yeast obtained from the novel strains. These yeasts are particularly useful for the comfort of the digestive tract and / or for the prevention and / or treatment of disorders of the digestive tract in humans or animals. Background technique [0003] The beneficial use of many microorganisms in the human digestive tract, as well as their nutritional benefits, have been described in the literature, eg in WO 2006 / 021965. [0004] These microorganisms are then usually designated in terms of the range of probiotics, which correspond to live microorganisms which, when administered in sufficient quantities, are capable of providing a health benefit to the host (in conjunction with FAO / WHO Expert Consultation Probiotics in food, FAO Food and nutrition paper Nr85, ISBN 92-5-105513-0). ...

Claims

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Application Information

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IPC IPC(8): C12N1/18A61K36/064A23L1/30
CPCC12R1/865A61K36/064A23L1/3016A23L33/14C12R2001/865C12N1/185A61P1/00A61P1/14A61P29/00C12N1/16C12N1/00
Inventor 让-吕克·西蒙乔治·皮涅德帕斯卡尔·范德克尔克奥韦达尼埃尔·普兰皮埃尔·德勒莫阿莱特·达尔弗耶-米肖阿德琳·西维尼恩
Owner LESAFFRE & CIE
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