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Method for testing efficacy of hog cholera lapinized virus live vaccine

An inspection method, the technology of swine fever rabbits, applied in the direction of biochemical equipment and methods, microbe determination/inspection, measuring devices, etc., can solve problems such as high labor intensity

Inactive Publication Date: 2013-07-03
CHINA INST OF VETERINARY DRUG CONTROL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It takes about 7 days for each batch of vaccines to be tested with rabbits, and the experimental results are easily affected by the breed and individual differences of rabbits, and the labor intensity is high; with pigs, 7 pigs are needed for each batch of vaccines, and rabbit bodies are required in advance. It takes about 3 weeks for the neutralization test to detect the antibody of the experimental pig
It can be seen that there are great deficiencies in the two effectiveness detection methods, and further improvement is urgently needed.

Method used

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  • Method for testing efficacy of hog cholera lapinized virus live vaccine
  • Method for testing efficacy of hog cholera lapinized virus live vaccine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0104] 1 Reagents and materials

[0105] 1.1 Reference virus of classical swine fever

[0106] The attenuated rabbit strain of classical swine fever virus (strain C) was identified, preserved and provided by the China Veterinary Drug Administration.

[0107] 1.2 Cell lines

[0108] Rabbit kidney cells (RK-13), according to the "Procedures" detection does not contain exogenous virus.

[0109] 1.3 Cell Culture Medium

[0110] 90% MEM+10% fetal bovine serum, pH 7.2.

[0111] 1.4 Cell Maintenance Solution

[0112] 98% MEM + 2% fetal bovine serum + double antibody (final concentration 100IU / ml each), pH 7.2.

[0113] 1.5 diluent

[0114] 99% MEM + double antibody (final concentration 100IU / ml each), pH 7.2.

[0115] 1.6 Fixative

[0116] 50% acetone-methanol solution (50% acetone + 50% methanol).

[0117] 1.7 Antibodies

[0118] Fluorescent antibody against swine-derived swine fever (provided by China Veterinary Drug Administration).

[0119] 2 Determination of virus tit...

Embodiment 2

[0144] 1 Reagents and materials

[0145] 1.1 Reference virus of classical swine fever

[0146] The attenuated rabbit strain of classical swine fever virus (strain C) was identified, preserved and provided by the China Veterinary Drug Administration.

[0147] 1.2 Cell lines

[0148] Rabbit kidney cells (RK-13), according to the "Procedures" detection does not contain exogenous virus.

[0149] 1.3 Cell Culture Medium

[0150] 90% MEM+10% fetal bovine serum, pH 7.2.

[0151] 1.4 Cell Maintenance Solution

[0152] 98% MEM + 2% fetal bovine serum + double antibody (final concentration 100IU / ml each), pH 7.2.

[0153] 1.5 diluent

[0154] 99% MEM + double antibody (final concentration 100IU / ml each), pH 7.2.

[0155] 1.6 Fixative

[0156] 50% acetone-methanol solution (50% acetone + 50% methanol).

[0157] 1.7 Antibodies

[0158] Swine-derived swine fever enzyme-labeled antibody (provided by China Veterinary Drug Administration).

[0159] 1.8 DAB chromogenic solution

[...

Embodiment 3

[0189] 1 Reagents and materials

[0190] 1.1 Reference virus of classical swine fever

[0191] The attenuated rabbit strain of classical swine fever virus (strain C) was identified, preserved and provided by the China Veterinary Drug Administration.

[0192] 1.2 Cell lines

[0193] Rabbit kidney cells (RK-13), according to the "Procedures" detection does not contain exogenous virus.

[0194] 1.3 Cell Culture Medium

[0195] 90% MEM+10% fetal bovine serum, pH 7.2.

[0196] 1.4 Cell Maintenance Solution

[0197] 98% MEM + 2% fetal bovine serum + double antibody (final concentration 100IU / ml each), pH 7.2.

[0198] 1.5 diluent

[0199] 99% MEM + double antibody (final concentration 100IU / ml each), pH 7.2.

[0200] 1.6 Fixative

[0201] 50% acetone-methanol solution (50% acetone + 50% methanol).

[0202] 1.7 Antibodies

[0203] Swine-derived swine fever antibody (provided by China Veterinary Drug Administration), fluorescently labeled secondary antibody (secondary antibo...

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PUM

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Abstract

The invention relates to a method for testing the efficacy of a hog cholera lapinized virus live vaccine, which comprises the following technical steps of: (1) establishing the method for testing the virus titer of the hog cholera lapinized virus live vaccine; (2) certifying the quantitative relationship between the virus titer and the rabbit test or the pig test through an experiment; and (3) performing in-vitro efficacy test and result determination on the vaccine by using the quantitative relationship. The method avoids the rabbit test or the pig test adopted by the conventional method fortesting the efficacy of the hog cholera lapinized virus live vaccine, and has the characteristics of avoiding the influence of animal species and individual difference, improving the accuracy of the efficacy test result, shortening the test time, reducing labor intensity and test cost and the like.

Description

technical field [0001] The invention relates to a method for testing the efficacy of a lanovirus attenuated live vaccine of hog fever, which belongs to the technical field of veterinary biological products. Background technique [0002] Classical Swine Fever (CSF) is a highly contagious and fatal swine infectious disease caused by Classical Swine Fever Virus (CSFV), which occurs in many countries and regions in the world. Industrial production has brought serious economic losses. The World Organization for Animal Health (OIE) lists swine fever as a statutory notifiable disease, and my country classifies it as a first-class animal infectious disease. [0003] For a long time, immunization with rabbitized attenuated live vaccine of classical swine fever has been the most effective and economical method of controlling classical swine fever widely used in the world. It has been nearly 50 years since the development and use of rabbitized attenuated live vaccines against swine f...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/569C12Q1/70C12Q1/02
Inventor 戴志红王在时关孚时蒋卉李翠陆连寿温芳赵耘秦玉明
Owner CHINA INST OF VETERINARY DRUG CONTROL
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