Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for testing efficacy of hog cholera lapinized virus live vaccine

A technology for swine fever rabbits and vaccines, which can be used in biochemical equipment and methods, microbial determination/inspection, measuring devices, etc., and can solve problems such as high labor intensity

Inactive Publication Date: 2010-12-15
CHINA INST OF VETERINARY DRUG CONTROL
View PDF4 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It takes about 7 days for each batch of vaccines to be tested with rabbits, and the experimental results are easily affected by the breed and individual differences of rabbits, and the labor intensity is high; with pigs, 7 pigs are needed for each batch of vaccines, and rabbit bodies are required in advance. It takes about 3 weeks for the neutralization test to detect the antibody of the experimental pig
It can be seen that there are great deficiencies in the two effectiveness detection methods, and further improvement is urgently needed.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for testing efficacy of hog cholera lapinized virus live vaccine
  • Method for testing efficacy of hog cholera lapinized virus live vaccine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0104] 1 Reagents and materials

[0105] 1.1 Reference virus of classical swine fever

[0106] The attenuated rabbit strain of classical swine fever virus (strain C) was identified, preserved and provided by the China Veterinary Drug Administration.

[0107] 1.2 Cell lines

[0108] Rabbit kidney cells (RK-13), according to the "Procedures" detection does not contain exogenous virus.

[0109] 1.3 Cell Culture Medium

[0110] 90% MEM+10% fetal bovine serum, pH 7.2.

[0111] 1.4 Cell Maintenance Solution

[0112] 98% MEM + 2% fetal bovine serum + double antibody (final concentration 100IU / ml each), pH 7.2.

[0113] 1.5 diluent

[0114] 99% MEM + double antibody (final concentration 100IU / ml each), pH 7.2.

[0115] 1.6 Fixative

[0116] 50% acetone-methanol solution (50% acetone + 50% methanol).

[0117] 1.7 Antibodies

[0118] Fluorescent antibody against swine-derived swine fever (provided by China Veterinary Drug Administration).

[0119] 2 Determination of virus tit...

Embodiment 2

[0144] 1 Reagents and materials

[0145] 1.1 Reference virus of classical swine fever

[0146] The attenuated rabbit strain of classical swine fever virus (strain C) was identified, preserved and provided by the China Veterinary Drug Administration.

[0147] 1.2 Cell lines

[0148] Rabbit kidney cells (RK-13), according to the "Procedures" detection does not contain exogenous virus.

[0149] 1.3 Cell Culture Medium

[0150] 90% MEM+10% fetal bovine serum, pH 7.2.

[0151] 1.4 Cell Maintenance Solution

[0152] 98% MEM + 2% fetal bovine serum + double antibody (final concentration 100IU / ml each), pH 7.2.

[0153] 1.5 diluent

[0154] 99% MEM + double antibody (final concentration 100IU / ml each), pH 7.2.

[0155] 1.6 Fixative

[0156] 50% acetone-methanol solution (50% acetone + 50% methanol).

[0157] 1.7 Antibodies

[0158] Swine-derived swine fever enzyme-labeled antibody (provided by China Veterinary Drug Administration).

[0159] 1.8 DAB chromogenic solution

[...

Embodiment 3

[0189] 1 Reagents and materials

[0190] 1.1 Reference virus of classical swine fever

[0191] The attenuated rabbit strain of classical swine fever virus (strain C) was identified, preserved and provided by the China Veterinary Drug Administration.

[0192] 1.2 Cell lines

[0193] Rabbit kidney cells (RK-13), according to the "Procedures" detection does not contain exogenous virus.

[0194] 1.3 Cell Culture Medium

[0195] 90% MEM+10% fetal bovine serum, pH 7.2.

[0196] 1.4 Cell Maintenance Solution

[0197] 98% MEM + 2% fetal bovine serum + double antibody (final concentration 100IU / ml each), pH 7.2.

[0198] 1.5 diluent

[0199] 99% MEM + double antibody (final concentration 100IU / ml each), pH 7.2.

[0200] 1.6 Fixative

[0201] 50% acetone-methanol solution (50% acetone + 50% methanol).

[0202] 1.7 Antibodies

[0203] Swine-derived swine fever antibody (provided by China Veterinary Drug Administration), fluorescently labeled secondary antibody (secondary antibo...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a method for testing the efficacy of a hog cholera lapinized virus live vaccine, which comprises the following technical steps of: (1) establishing the method for testing the virus titer of the hog cholera lapinized virus live vaccine; (2) certifying the quantitative relationship between the virus titer and the rabbit test or the pig test through an experiment; and (3) performing in-vitro efficacy test and result determination on the vaccine by using the quantitative relationship. The method avoids the rabbit test or the pig test adopted by the conventional method fortesting the efficacy of the hog cholera lapinized virus live vaccine, and has the characteristics of avoiding the influence of animal species and individual difference, improving the accuracy of the efficacy test result, shortening the test time, reducing labor intensity and test cost and the like.

Description

technical field [0001] The invention relates to a method for testing the efficacy of a lanovirus attenuated live vaccine of hog fever, which belongs to the technical field of veterinary biological products. Background technique [0002] Classical Swine Fever (CSF) is a highly contagious and fatal swine infectious disease caused by Classical Swine Fever Virus (CSFV), which occurs in many countries and regions in the world. Industrial production has brought serious economic losses. The World Organization for Animal Health (OIE) lists swine fever as a statutory notifiable disease, and my country classifies it as a first-class animal infectious disease. [0003] For a long time, immunization with rabbitized attenuated live vaccine of classical swine fever has been the most effective and economical method of controlling classical swine fever widely used in the world. It has been nearly 50 years since the development and use of rabbitized attenuated live vaccines against swine f...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569C12Q1/70C12Q1/02
Inventor 戴志红王在时关孚时蒋卉李翠陆连寿温芳赵耘秦玉明
Owner CHINA INST OF VETERINARY DRUG CONTROL
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com