Derivative L of rhodamine B, preparation thereof and use thereof

Abstract

The invention relates to fluorescent probe detection, in particular to a derivative L of rhodamine B, preparation thereof and use thereof. The derivative L of the rhodamine B has a structural formula (I). The preparation method of the derivative L of the rhodamine B comprises: firstly, reacting the product of the hydrazinolysis of the rhodamine B with glyoxal to obtain an intermediate compound; and secondly, reacting the intermediate compound with benzyldithiocarbazate in a molar ratio of 1:1 in absolute ethanol under a refluxing condition for 4 to 6 hours, cooling, filtering precipitated solid, washing with absolute ethanol and ethyl ether in turn and drying under vacuum to obtain a target compound. The derivative L of rhodamine B can serve as a fluorescent probe. In the invention, the derivative L of rhodamine B is obtained by an effective synthesis means, the copper ion selectivity is high, and the detection of copper ions can be realized on the basis of optimized experimental conditions.

Description

technical field The invention relates to fluorescent probe detection, in particular to a rhodamine B derivative and its preparation and application. Background technique Rhodamine B derivatives have excellent optical properties and are widely used in metal ion fluorescent probes. Fluorescent probes based on rhodamine B derivatives have been applied to the detection of mercury ions, lead ions, iron ions, chromium ions, and copper ions. Among the reported fluorescent molecular probes, most of them have the following disadvantages: complex synthesis and separation, large interference of other coexisting ions in the test process, high detection limit, and response signals of fluorescent quenching probes, etc., which are harmful to metal ions Detection has brought great influence and inconvenience. Contents of the invention The object of the present invention is to provide a rhodamine B derivative and its preparation and application. To achieve the above object, the techn...

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Problems solved by technology

Among the reported fluorescent molecular probes, most of them have the following disadvantages: complex synthesis and separation, large interference of other coexisting ions in the test process, high detection limit, and response signals of fluorescent quenching probes, etc., which are harmful to metal ions The detection has brought great impact and inconvenience

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