Preparation method, application and QC (Quality Control) detection method of platycodon grandiflorum active site

Abstract

The invention provides a preparation method and a new application of a platycodon grandiflorum active site, in particular the application of a platycodon grandiflorum active site in an antineoplastic drug. The invention also relates to two new triterpenoidal saponin compounds (compounds A and B) separated from the platycodon grandiflorum active site by a phytochemical separation way and a preparation method thereof. The invention further provides a detection method for QC (Quality Control) of the platycodon grandiflorum by platycodin D, polygala saponin D2, platycodin D3, deapiose platycodin D3, platycodin D2, deapiose platycodin D2, deapiose platycodin E and platycodin E, and the method has excellent repeatability and is capable of comprehensively evaluating the quality of the platycodon grandiflorum, platycodon grandiflorum extracts and the platycodon grandiflorum active site.

Description

technical field The invention relates to the field of medicine and a chromatographic detection method, in particular to the preparation of the active part of Platycodon grandiflora, the extraction and separation method of a new compound and the identification of its chemical structure; the application of the active part of Platycodon grandiflorum in antitumor drugs; the determination of the extract of Platycodon grandiflorum and its Method of monomeric saponin content in the active site. Background technique Platycodon grandiflorum (Jacq.) A.DC is a perennial herb of Platycodonaceae. Its medicinal part is the root. It is one of the forty traditional Chinese medicines commonly used in my country. It is widely distributed in my country, the Korean Peninsula and Japan. Platycodon grandiflorum is bitter in nature. , pungent, flat, return the lung meridian, have the effect of opening the lung qi, eliminating phlegm and draining pus. It is commonly used in diseases such as exogeno...

AI Technical Summary

Problems solved by technology

The 2005 edition of "Chinese Pharmacopoeia" uses the gravimetric method to determine the content of total saponins in Platycodon grandiflorum, and stipulates that the content of total saponins in medicinal materials should not be less than 6%. This method is cumbersome to operate, with large measurement errors and poor reproducibility

Using monomeric saponins to analyze the quality of platycodon saponins, as an index to evaluate the quality of platycodon grandiflora or to carry out improved breeding, lacks integrity and scientificity

At the same time, in the determination of platycodon saponin D at home and abroad, platycodon saponin D and platycodon saponin D 2 Not completely separated, resulting in inaccurate determination of its content (Zhu Danni, Zhi Hui, Gao Shanlin. The HPLC-ElSD method is used to determine the content of platycodon saponin D in Platycodon grandiflorum. Journal of Plant Resources and Environment, 2001, 10 (4): 11-13 . Zhang Zhenling, Yang Hailing, Zhang Hongwei, etc. Effect of processing on platycodon saponin D content in platycodon grandiflorum. Chinese patent medicine, 2008, 30(4): 554-556. Guo Li, Xiao Yongqing, Zhang Cun, etc. Platycodon platycodon saponin D in medicinal materials Qualitative and quantitative method research. Journal of Beijing University of Traditional Chinese Medicine, 2007, 30 (3): 200-209. Huang Ying, Shi Chunlei, Liu Moxiang, etc. HPLC-ELSD method for the determination of eight kinds of platycodon saponins in platycodon root slices. Journal of Yangzhou University ( Natural Science Edition), 2008, 11(4): 41-43.)

Images