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Benazepril hydrochloride composition with effect of reducing blood pressure and preparation method thereof

A technology of benazepril hydrochloride and composition, applied in the field of benazepril hydrochloride composition and preparation thereof, can solve the problems of limited clinical application, unsatisfactory control of hypertension and the like

Inactive Publication Date: 2010-12-01
BEIJING WBL PEKING UNIV BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the effects of antihypertensive drugs on metabolism and target organ damage are still controversial, and most of them have certain side effects, which partially limit their clinical application.
Make the control of high blood pressure unsatisfactory

Method used

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  • Benazepril hydrochloride composition with effect of reducing blood pressure and preparation method thereof
  • Benazepril hydrochloride composition with effect of reducing blood pressure and preparation method thereof
  • Benazepril hydrochloride composition with effect of reducing blood pressure and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0054] Embodiment 1: Composition solid fermentation of benazepril hydrochloride

[0055] Slant strain culture: select Aspergillus CICC 2436 for strain culture, add agar to wort at 4 degrees to prepare a medium with a Ph of 7, so that each ml of medium contains 0.05g of agar; sterilize at 110°C, After sterilizing, cool down, prepare slant and inoculate it, and cultivate it at 30°C for 5 days as the slant strain; solid fermentation culture: mix bran and wort in a weight-to-volume ratio of 1:1, and mix well Under the condition of 110°C, sterilize for 30 minutes, cool down after sterilization, insert the slant bacteria so that the volume of the slant bacteria accounts for 8% of the culture medium, cultivate at 30°C for 3 days, and add to the cultivated bran Soak in 0.3M ph7pbs buffer overnight to obtain a solid fermentation medium; preparation of crude enzyme solution: add ammonium sulfate to the solid fermentation medium until saturated, stir evenly until completely dissolved, pl...

Embodiment 2

[0056] Embodiment 2: Composition solid fermentation of benazepril hydrochloride

[0057] Slant strain culture: select Aspergillus CICC 2436 for strain culture, add agar to wort at 4 degrees to prepare a Ph 6 medium, so that each ml medium contains 0.03g of agar; sterilize at 105°C, After cooling, the slant is prepared and inoculated by the conventional method, and cultivated at 35°C for 4 days as the slant strain; solid fermentation culture: mix the bran and wort in a weight-to-volume ratio of 1:1.5, and mix well Finally, under the condition of 105°C, sterilize for 35 minutes, cool down after sterilization, insert the slant bacteria so that the volume of the slant bacteria accounts for 6% of the medium volume, and cultivate for 2 days at 35°C, and the cultivated bran Add 0.5M ph7pbs buffer solution to soak overnight to obtain solid fermentation medium; preparation of crude enzyme solution: add ammonium sulfate to solid fermentation medium to saturation, stir evenly until compl...

Embodiment 3

[0058] Embodiment 3: Composition solid fermentation of benazepril hydrochloride

[0059] Slant strain culture: select Aspergillus CICC 2436 for strain culture, add agar to wort at 4 degrees to prepare a Ph 7 medium, so that each ml medium contains 0.08g of agar; sterilize at 115°C, Cooling after the bacteria, the slant is prepared and inoculated by the conventional method, and cultured at 25°C for 6 days as the slant strain; solid fermentation culture: mix the bran and wort according to the ratio of weight to volume of 1:1.8, and mix well Finally, under the condition of 120°C, sterilize for 25 minutes, cool after sterilization, insert the slant strains so that the volume of the slant strains accounts for 9% of the medium volume, and cultivate them for 2 days at 25°C. Add 0.4M ph7pbs buffer solution to soak overnight to obtain solid fermentation medium; preparation of crude enzyme solution: add ammonium sulfate to solid fermentation medium until saturated, stir evenly until com...

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Abstract

The invention discloses a benazepril hydrochloride composition with effect of reducing blood pressure and a preparation method thereof. The preparation process comprises a solid fermentation method and a liquid fermentation method. Aspergillus CICC 2436 produces enzyme to hydrolyze benazepril hydrochloride, wherein the structure of part of benazepril hydrochloride is changed, namely an analog of benazepril hydrochloride. The original benazepril hydrochloride forms a composition containing the benazepril hydrochloride and the analog of the benazepril hydrochloride under the action of the enzyme, and the level of reducing the blood pressure by using the composition is superior to that of the benazepril hydrochloride with the same concentration.

Description

technical field [0001] The invention relates to a composition, in particular to a benazepril hydrochloride composition with hypotensive effect and a preparation method thereof. Background technique [0002] At present, there are roughly 6 categories of drugs for the treatment of hypertension in modern medicine: 1. Diuretics 2. α receptor blockers 3. β receptor blockers 4. Calcium ion antagonists 5. Angiotensin converting enzyme inhibitors 6 . Angiotensin II receptor antagonists. In recent years, with the development of large-scale antihypertensive clinical trials and the progress of cardiovascular molecular biology research, the traditional understanding of hypertension has been updated, and evidence-based medicine has become a consensus. Hypertension is not only a disease of abnormal hemodynamics, but also accompanied by disorders of fat and glucose metabolism and adverse remodeling of target organs such as the heart, brain, and kidney. Therefore, the treatment should imp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P17/10A61K31/55A61P9/12C12R1/66
Inventor 段震文郭树仁巩金妹薛岚刘春丽
Owner BEIJING WBL PEKING UNIV BIOTECH
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