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Method for extracting streptomyces antibacterial product

An extraction method, streptomyces technology, applied in botany equipment and methods, chemicals for biological control, fungicides, etc., can solve the problems of high cost and unsatisfactory effect, and achieve storage resistance, excellent fermentation properties, Excellent adsorption selectivity

Inactive Publication Date: 2013-01-23
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Domestic researchers mostly focus on the improvement of toxin detoxification technology and the optimization of detection methods. In the past, the methods for preventing and controlling Aspergillus and its toxins from contaminating grain and feed mainly used physical, chemical and biological methods to remove contaminated food. Aspergillus and toxins are not only costly, but also unsatisfactory

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  • Method for extracting streptomyces antibacterial product
  • Method for extracting streptomyces antibacterial product

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Experimental program
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Effect test

Embodiment 1

[0040](1) Streptomyces S24 culture and fermentation: After the strains stored on the slant are activated, transfer to the plate PDA medium to grow for 48 hours, punch holes and inoculate into the seed medium, seed culture conditions: temperature 28 ° C, shaker speed 200 rpm , culture time 24 hours; seed culture liquid is inoculated in fermentation medium by 2%, fermentation culture condition: temperature 28 ℃, shaker speed 200 rev / min, liquid volume of shaking bottle 50ml / 250ml, culture time 96 hours; after fermentation , collect the fermentation broth, centrifuge at 4200 rpm in a low-speed large-capacity centrifuge for 30 minutes, collect the fermentation supernatant, the volume is 2400ml, and the bioassay titer is 4563 μg / mL;

[0041] (2) Macroporous adsorption resin AB-8 adsorbs antibacterial products: the supernatant after the centrifugation of the adsorption resin AB-8 and the fermentation broth is mixed in a ratio of m / V=1:20, and oscillating and adsorbing at 150 rpm for ...

Embodiment 2

[0045] (1) Streptomyces S24 culture and fermentation: After the strains stored on the slant are activated, transfer to the plate PDA medium to grow for 56 hours, punch holes and inoculate into the seed medium, seed culture conditions: temperature 28 ° C, shaker speed 200 rpm , culture time 24 hours; seed culture liquid is inoculated in fermentation medium by 2%, fermentation culture condition: temperature 28 ℃, shaking table speed 200 rev / min, shaking bottle liquid volume 100ml / 500ml, culture time 96 hours; after fermentation , collect the fermentation broth, centrifuge at 4200 rpm in a low-speed large-capacity centrifuge for 30 minutes, collect the fermentation supernatant, the volume is 4600ml, and the bioassay titer is 4325.5 μg / mL;

[0046] (2) Macroporous adsorption resin AB-8 adsorbs antibacterial products: the supernatant after the centrifugation of the adsorption resin AB-8 and the fermentation broth is mixed in a ratio of m / V=1:20, and oscillating and adsorbing at 150 ...

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Abstract

The invention relates to a method for extracting a streptomyces antibacterial product, and belongs to the technical field of biology. Streptomyces sp S24 is activated and fermented; the fermentation solution is sterilized, centrifuged, absorbed by macroporous absorption resin AB-8 and desorbed by 75 to 85 percent acetone; and a solvent is reclaimed from the desorption solution, and the desorptionsolution is evaporated and dried to obtain an antibacterial product extract. Experiments prove that the antibacterial product has broad-spectrum antibacterial activity for main mildewing fungi such as aspergillus flavus, aspergillus niger, aspergillus ochraceus and aspergillus fumigatus in food and feed and human pathogenic fungi such as aspergillus flavus and aspergillus fumigatus. Because the antibacterial product provided by the invention has obvious inhibiting effect on various food and geed mildewing fungi and human pathogenic fungi, the antibacterial product shows efficient broad-spectrum properties; and the method for extracting the antibacterial product is simple and convenient, has low cost, and has high practical application value and broad market prospect on the aspects of foodpreservative, feed additive and medicaments.

Description

(1) Technical field [0001] The invention relates to a method for extracting an antibacterial product produced by using a macroporous adsorption resin from a Streptomyces S24 fermented liquid, and belongs to the field of biotechnology. Specifically, it is a method for extracting antibacterial products from Streptomyces S24 fermentation broth. (2) Background technology [0002] The purpose of the present invention is to extract the antibacterial product produced by the fermentation of Streptomyces S24 to prevent and control the main mildew fungi in grain and feed, as well as human pathogenic fungi such as Aspergillus flavus and Aspergillus fumigatus. [0003] Contamination of food and feed by Aspergillus fungi can lead to aspergillosis in humans and animals, and the toxin produced has the effects of inducing mutation, suppressing immunity and carcinogenesis. Since the 1960s, people have conducted extensive research on it and are committed to its effective control. Domestic re...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/02A61K35/74A61P31/10A01P3/00A61K35/741
Inventor 刘训理周启升毛志泉张楠曹宁宁张莎莎
Owner SHANDONG AGRICULTURAL UNIVERSITY
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