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Herbicide resistance gene and application thereof

A technology for resistance genes and herbicides, applied in the field of genetic engineering, can solve the problems of difficult control of the expression level of multiple genes, unsatisfactory resistance effect, and complexity, and achieves the ability to improve the ability to resist salt and other adversity stresses, improve the use efficiency, and expand the The effect of the herbicide spectrum

Inactive Publication Date: 2010-11-24
HEBEI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The above three strategies have obtained herbicide-resistant transgenic plants to varying degrees, but their resistance effects are not ideal, especially the latter two strategies are very complicated to implement, and the expression level of multiple genes is difficult to control

Method used

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  • Herbicide resistance gene and application thereof
  • Herbicide resistance gene and application thereof
  • Herbicide resistance gene and application thereof

Examples

Experimental program
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Effect test

Embodiment Construction

[0035] The following examples illustrate the invention in detail. The molecular biology and biochemical methods used, such as the preparation of expression components including promoters, target genes, and terminators, the construction of plant transformation plasmids, the technology of plant transformation of target genes, and plant regeneration technologies, are all mature and established. Known technology; "Current Protocols in Molecular Biology" written by Ausubel, published by John Wiley and Sons, and "Molecular Cloning: Laboratory Manual" written by J.Sambrook, etc., published by Cold Spring Harbor Laboratory Press (2001), 3rd ED . and other documents have detailed instructions.

[0036] Part of the medium used in the following examples, the mother liquor formula are as follows:

[0037] (1) YEB medium: beef extract 5g / L, yeast extract 1g / L, peptone 5g / L, sucrose 5g / L, MgSO 4 2mmol / L, pH7.2, solid medium containing agar 15g / L.

[0038] (2) Tobacco differentiation med...

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Abstract

The invention discloses a herbicide resistance gene and an application thereof. The herbicide resistance gene comprises a base sequence in SEQ ID NO:1. The invention also discloses a herbicide resistance protein and a plant expression plasmid, wherein the herbicide resistance protein contains an amino acid sequence in SEQ ID NO:2, and the plant expression plasmid contains the herbicide resistancegene. The herbicide resistance gene is a resistance gene of an HPPD inhibitor type herbicide and is led into plant cells through the gene engineering technology to prepare a transgenic plant which has natural resistance to the HPPD inhibitor type herbicide and simultaneously has high stress resistance such as salt resistance and the like.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, in particular to a herbicide resistance gene, a protein encoded by it, and a transgenic plasmid prepared or cultivated using it, a genetically engineered bacterial strain, a herbicide resistant plant and a highly stress-resistant plant. Background technique [0002] Currently, the development of non-selective broad-spectrum herbicides and their corresponding resistant plants is one of the main modes of weed control. Inhibitors of 4-hydroxyphenylpyruvate dioxygenase (HPPD; EC 1.13.11.27) are a newly developed family of albino herbicides, and the target enzyme HPPD is plant plastoquinone Key synthetic enzymes of biosynthetic pathways. Its mechanism of action is that, through the inhibition of HPPD enzyme, it reduces plastoquinone and synthesis, which further leads to the reduction of carotenoid biosynthesis in thylakoids, causing leaf albino symptoms, so as to achieve the purpose of we...

Claims

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Application Information

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IPC IPC(8): C12N15/29C07K14/415C12N15/82C12N1/21A01H4/00A01H5/00C12R1/01
Inventor 梁玉玲于静娟管延英吴科瀛
Owner HEBEI UNIVERSITY
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