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Method for preparing high-purity ursolic acid from salvia

A ursolic acid, high-purity technology, applied in the field of natural medicine, can solve the problems of small sample loading, unfavorable industrial production, low yield and the like, and achieve the effects of small processing volume, easy processing volume and high yield

Inactive Publication Date: 2010-11-24
曾超
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Ursolic acid widely exists in leaves and fruits of specific families and genera. Different raw materials are separated and purified to obtain ursolic acid with high purity, mainly by means of column chromatography or macroporous resin enrichment, although column chromatography can be very Good separation of ursolic acid and similar impurities, but this method has a small amount of sample and low yield, which is not conducive to large-scale industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Get 1Kg of dry sage leaves, crush them, pass through a 20-mesh sieve, add 12L of petroleum ether, soak for 24 hours at room temperature, filter out the petroleum ether, dry the leaf powder, soak the dry leaf powder with 2L of acetone at room temperature for 10 hours, and then pour it into Continue to add 8L of acetone to the leaf powder, reflux extraction for 2 hours, repeat 2-3 times, combine the acetone extract, filter, concentrate the extract to 3L, filter while hot, and place the clarified filtrate at 4°C for 24 hours. A large amount of yellow-white precipitate was precipitated, which was crude ursolic acid, which was filtered by suction and dried in vacuum. The crude ursolic acid was weighed to be 97g. Wash the crude ursolic acid with 600ml of chloroform and petroleum ether mixed solution with a volume ratio of 1:2, filter with suction, vacuum-dry the filter cake, weigh 74g, add 500ml of acetone and 5g of activated carbon to the filter cake, and heat at 50-60°C Sti...

Embodiment 2

[0016] Get 1Kg of dry sage leaves, crush them, pass through a 20-mesh sieve, add 12L of petroleum ether, soak for 24 hours at room temperature, filter out the petroleum ether, dry the leaf powder, soak the dry leaf powder with 2L of acetone at room temperature for 10 hours, and then pour it into Continue to add 8L of acetone to the leaf powder, reflux extraction for 2 hours, repeat 2-3 times, combine the acetone extract, filter, concentrate the extract to 1L, filter while hot, and place the clarified filtrate at 4°C for 24 hours. A large amount of yellow precipitate was precipitated, which was the crude product of ursolic acid. It was suction filtered and dried in vacuum. The crude product of ursolic acid was weighed to be 164g. Recrystallize once with 800ml of acetone, filter with suction, dry in vacuo, then dissolve the crude product with 400ml of ethanol-acetic acid mixed solution with a volume ratio of 1:1, add 4g of activated carbon, stir at 50-60°C for 1 hour, filter out ...

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Abstract

The invention discloses a method for preparing high-purity ursolic acid from salvia, belonging to the natural medicine technical field. The method comprises the following steps: drying and pulverizing salvia leaves; dipping leaf meal with petroleum ether to remove grease and pigment; filtering out the petroleum ether and then blow-drying the leaf meal; carrying out thermal reflux extraction on the dried leaf meal with acetone for 2-3 times for hours; filtering, merging, concentrating and cooling extracting solution, keeping standing and then separating out precipitate to obtain a crude ursolic acid product; and decolorizing the crude ursolic acid product by utilizing activated carbon, washing, removing impurities, and recrystallizing with a hydrophilic solvent to obtain the high-purity ursolic acid. The method has the advantages of small 'three wastes' treatment capacity, easy 'three wastes' treatment, recyclable acetone, lower cost, high yield and high purity of the obtained ursolic acid, simplicity, convenience and fastness; and the method is mainly designed according to the character that the ursolic acid is insoluble in water and the petroleum ether but soluble in acetone. Wave spectrum analysis result shows that the obtained product is in accordance with the standard of the ursolic acid.

Description

technical field [0001] The invention belongs to the technical field of natural medicines, and in particular relates to a method for extracting and purifying the main active ingredient ursolic acid from sage. technical background [0002] Sage is medicinal sage, also known as sage. It is a perennial herb of Lamiaceae. The Latin scientific name is salvia officinalis. It has broad oval leaves and the plants are clustered. The leaves grow in pairs and are oval in color. , the surface of the leaves has concave and convex textures, the fragrance is pungent and strong, and the lavender flowers bloom in summer, the growth is strong, and it is resistant to diseases and insect pests. Native to the Mediterranean region. The dried or fresh leaves are used as a seasoning for many foods, especially as a stuffing for sausages, poultry, and pork. Many species and varieties of medicinal sage are cultivated wild in many parts of the world. In my country, it is widely distributed in Zhejian...

Claims

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Application Information

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IPC IPC(8): C07J63/00
Inventor 李靖靖司福亭董彩霞高建炳周晓莉郭林高广颖司方博岳福兴付鸿王文武王宇飞华慧颖曾超
Owner 曾超
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