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Aseptic and rapid propagation method of dendrobium devonianum seeds

A technology of dendrobium dendrobium and seeds, which is applied in the field of seedling cultivation of medicinal dendrobium, can solve the problems of slow propagation speed of seedlings, decreased resistance to stress, and easy infection of wounds, and achieve the effect of increasing the propagation speed and value-added multiples

Inactive Publication Date: 2010-11-24
YUNNAN DEHONG TROPICAL AGRI RES INST
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Problems solved by technology

In recent years, people have artificially propagated Dendrobium dendrobii by traditional ramet cultivation and cuttings. However, due to the strong dependence on the mother plant, not only the loss of raw materials is large, but also the wounds are susceptible to diseases, and the stress resistance is reduced, resulting in seedling propagation. Slow speed, irregular seedlings, low survival rate

Method used

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Experimental program
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Effect test

Embodiment Construction

[0006] Select mature uncracked capsules of Dendrobium dendrobium as explants, and the specific method includes the following steps: (1) selection and disinfection of explants: select mature uncracked capsules, soak them in 0.1% ethanol for 1 min with 75% alcohol Disinfect in mercuric chloride solution for 15 to 20 minutes, and then rinse with sterile water for 5 to 6 times; (2) Germination culture: cut the capsule skin, inoculate the golden yellow powdery embryos on the germination medium, and the germination medium is flower Bao No. 1 (N:P:K=7:6:19) 3 g / L + LH (hydrolyzed milk protein) 1 g / L + sucrose 25 g / L, germination medium pH 5.8-6.2, culture temperature 25 ~27°C, light intensity 1500-2000Lx, 12 hours of light per day, 40-45 days of culture time, forming green protocorms with a diameter of about 1mm; (3) Proliferation culture: inoculate the protocorms on the proliferation medium, MS + 6-BA (6-benzyl adenine) 0.1-1.0 mg / L + NAA (α-naphthalene acetic acid) 0.1-1.0 mg / L + s...

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Abstract

The invention discloses an aseptic and rapid propagation method of dendrobium devonianum seeds, which relates to the technical field of biological engineering. The invention is realized through the following scheme comprising the following steps of: by using the mature and uncracked capsule of dendrobium devonianum as an explant, firstly cutting a peel after disinfection processing, and carrying out germination cultivation on a seed embryo to from a large amount of protocorms; then carrying out multiplication cultivation on the protocorms to form a large amount of cluster buds or protocorm-like mixed tissues; then carrying out strong seedling rooting cultivation on the cluster buds to form a large amount of integral plant test-tube seedlings with roots, stems and leaves; and finally obtaining a large amount of high-quality seedlings after the domestication and the transplantation of the test-tube seedlings. The method can improve the seedling propagation speed and the multiplication multiple of the dendrobium devonianum, achieves full and strong seedlings and is beneficial to factory production.

Description

technical field [0001] The invention relates to a method for cultivating medicinal dendrobium seedlings, in particular to a method for aseptic rapid propagation of dendrobium dendrobium seeds. Background technique [0002] Dendrobium is a traditional precious Chinese medicinal material in my country. Dendrobium dendrobium is a good product among the Dendrobium species. It is mostly epiphytic on alpine rocks and trees. In recent years, due to the good market of Dendrobium dendrobium, people have plundered it, and the wild resources are on the verge of exhausted. In recent years, people have artificially propagated Dendrobium dendrobii by traditional ramet cultivation and cuttings. However, due to the strong dependence on the mother plant, not only the loss of raw materials is large, but also the wounds are susceptible to diseases, and the stress resistance is reduced, resulting in seedling propagation. The speed is slow, the seedlings are not neat, and the survival rate is lo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G31/00
Inventor 白燕冰高燕李守岭季丽坤
Owner YUNNAN DEHONG TROPICAL AGRI RES INST
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