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Method for producing multifunctional antibacterial peptide

A production method and antibacterial peptide technology are applied in the field of preparation of antibacterial peptide products to achieve the effects of facilitating promotion, reducing a large number of antibiotics, and huge economic benefits

Inactive Publication Date: 2010-11-17
BANGCHENG FORAGE SCI & TECH SHANGHAI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, there are certain limitations in the nutrition produced by fermentation using this method.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1) Raw material processing: Soybean meal and corn are respectively crushed into particles with a diameter of 0.8-1mm.

[0027] 2) Raw material ratio: soybean meal 300kg, corn 700kg.

[0028] 3) Sterilization of solid medium: Put the above soybean meal and corn into a double-cone sterilizer and mix them, and pass in steam to make the steam in the sterilizer reach 0.1 MPa, and maintain it for 40 minutes, and then cool to 30°C.

[0029] 4) Configuration of nutrient solution for solid-state fermentation: 20kg of peptone, 10kg of yeast extract, add 350kg of water into the blending tank to make it fully mixed.

[0030] 5) Aerobic inoculation and fermentation: extract 12.5L of pre-cultured yeast and Streptococcus faecalis and mix them with 175L of solid-state fermentation nutrient solution, and then mix them with 500kg of the above-mentioned sterilized and cooled soybean meal and corn in an arrow-type inoculator. Mix well, and then put it into an aerobic fermentation containe...

Embodiment 2

[0035] 1) Raw material processing: crush soybean meal and corn into particles with a diameter of 0.8-1 mm.

[0036] 2) Raw material ratio: soybean meal 400kg, corn 600kg.

[0037] 3) Sterilization of the solid medium: put the above soybean meal and corn into a double-cone sterilizer and mix them, and feed steam to make the steam in the sterilizer reach 0.1 MPa and maintain it for 40 minutes. Cool to 33°C.

[0038] 4) Configuration of solid-state fermentation nutrient solution: 20kg of peptone, 10kg of yeast extract, add 350kg of water into the blending tank to make it fully mixed.

[0039] 5) Aerobic inoculation and fermentation: extract 12.5L of yeast and Streptococcus faecalis cultured in advance and mix them with 175L of solid-state fermentation nutrient solution, and then mix them with 500kg of the above-mentioned sterilized and cooled soybean meal and corn in an arrow-type inoculator. Mixed, and then put into an aerobic fermentation vessel for normal temperature aerobic...

Embodiment 3

[0044] 1) Raw material processing: crush soybean meal and corn into particles with a diameter of 0.8-1 mm.

[0045] 2) Raw material ratio: soybean meal 500kg, corn 500kg.

[0046] 3) Sterilization of solid medium: Put the above soybean meal and corn into a double-cone sterilizer and mix them, and pass in steam to make the steam in the sterilizer reach 0.1 MPa, and maintain it for 40 minutes, and then cool to 35°C.

[0047] 4) Configuration of nutrient solution for solid-state fermentation: 20kg of peptone, 10kg of yeast extract, and add 350kg of water into the mixing tank to make it fully mixed.

[0048]5) Aerobic inoculation and fermentation: extract 12.5L of yeast and Streptococcus faecalis cultured in advance and mix them with 175L of solid-state fermentation nutrient solution, and then mix them with 500kg of the above-mentioned sterilized and cooled soybean meal and corn in an arrow-type inoculator. Mix, and put into the aerobic fermentation container to carry out normal ...

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Abstract

The invention discloses a method for producing a multifunctional antibacterial peptide. The method comprises the following steps of: 1) treating raw materials; 2) preparing the raw materials; 3) sterilizing a solid medium; 4) preparing nutrient solution for solid state fermentation; 5) performing aerobic inoculation and fermentation; 6) performing anaerobic inoculation and fermentation; and 7) drying and sieving to obtain the finished product of the antibacterial peptide. The measured antibacterial peptide content of the finished product of the antibacterial peptide is 100-400 IU per gram and the total number of probiotics is 10-40 billion per gram. The solid state fermentation is performed by four strains including complex yeast, bifidobacterium, lactobacillus and streptococcus faecalis, so the shortcoming that the antibacterial peptide is produced by a single strain is overcome, and a large number of probiotics are maintained while the multifunctional antibacterial peptide is produced; and moreover, the method realizes the coexistence of the large number of probiotics and the antibacterial peptide while keeping the antibacterial activity. The antibacterial peptide produced by the method is applicable to the feed additive industry.

Description

technical field [0001] The invention relates to the preparation of antimicrobial peptide products, in particular to a method for producing multifunctional antimicrobial peptides. The antimicrobial peptides prepared by the method of the invention can be used in the feed additive industry. Background technique [0002] With the development of modern intensive and large-scale farming, the risk of livestock, poultry and aquatic animals being infected with various diseases has greatly increased. In order to prevent animal diseases, promote animal growth and improve feed utilization, a large number of drugs such as antibiotics are used as feed additives. With the long-term and large-scale use of antibiotic additives, their negative effects are increasingly exposed. The main reason is that the problem of drug-resistant strains and endogenous infection caused by long-term use of antibiotics has always troubled farmers. The potential impact of drug-resistant strains on human health ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P39/00C12P21/00A23K1/16C12R1/85C12R1/46C12R1/225C12R1/01
Inventor 陆克文欧阳亮丰涛
Owner BANGCHENG FORAGE SCI & TECH SHANGHAI
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