Two-channel detection card of troponin I and troponin T

A troponin and detection card technology, applied in biological testing, material inspection products, etc., can solve the problem of no simultaneous detection and detection equipment, etc., and achieve the effects of saving detection costs, easy preparation, and high sensitivity

Inactive Publication Date: 2010-11-10
WUXI ANKANG BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, there is no detection device that detects these two substances at the same time

Method used

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  • Two-channel detection card of troponin I and troponin T
  • Two-channel detection card of troponin I and troponin T
  • Two-channel detection card of troponin I and troponin T

Examples

Experimental program
Comparison scheme
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Embodiment Construction

[0013] Referring to the accompanying drawings for further description in conjunction with specific embodiments:

[0014] A dual-channel detection card for troponin I and troponin T, which is provided with a test strip 2 in the detection card casing 1, the structure of the detection card casing 1 and the setting of the test strip 2 refer to the attached figure 1 , The detection card shell 1 is a long flat shell-shaped shell with a length of 7cm, a width of 2cm, a thickness of 0.5cm, and a wall thickness of the thin shell of 0.1cm, made of engineering plastics. The detection card casing 1 is composed of two halves of a casing cover and a casing seat, and a detection window 3 and a sample injection hole 4 are arranged on the casing cover of the detection card casing 1 . The test strip 2 is placed in the shell seat of the test card shell 1 .

[0015] The test strip 2 is a thin strip with a multi-layer structure, the length of the strip is about 6.0cm, the width of the strip is ab...

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Abstract

The invention relates to a two-channel detection card of troponin I and troponin T, which belongs to the technical field of blood detection. A test strip is arranged in a casing of the detection card and is stuck with a cellulose nitrate film by the middle part of a supporting back plate; a water absorption film and a sample pad are respectively stuck on the two end parts of the test strip; the inner end of the test strip is respectively in lap joint with one end of the cellulose nitrate film; two sections of colloidal gold films are clamped and stuck between the lap joint parts of the sample pad and the cellulose nitrate film and are respectively glass fibre films containing a monoclonal-antibody colloidal gold marker of the troponin I and a monoclonal-antibody colloidal gold marker of the troponin T; two detection strips are arranged on the cellulose nitrate film and contain an antibody resisting the troponin I and an antibody resisting the troponin T respectively, and another mass hole strip contains an anti-mouse antibody. The sample pad and the cellulose nitrate film are over against a sample loading hole and a detection window hole respectively. The invention has the advantage that the troponin I and the troponin T which are contained in the serum or the plasma of a patient can be detected simultaneously. The detection card is easy to prepare, saves the detection expense and has convenient use, quick detection, high sensitivity and accurate result.

Description

technical field [0001] A dual-channel detection card for simultaneously detecting troponin I and troponin T relates to a device for simultaneously detecting troponin and belongs to the technical field of blood testing. Background technique [0002] Troponin (Tn) is a regulatory protein related to myocardial contraction, regulating the contraction and relaxation of the myocardium. It consists of three subunits: troponin I (tropomyosin inhibitory component, TnI), troponin T (tropomyosin-binding component, TnT), and troponin C (calinmbinding component, TnC). TnI has a molecular weight of 21kD and is an inhibitory subunit of myofibrillar ATPase, which can inhibit the coupling of myosin and actin and relax skeletal or cardiac muscle. TnT, with a molecular weight of 37kD, is a subunit that binds to tropomyosin, which connects troponin C and troponin I to actin and tropomyosin, thereby playing an intermediary role in muscle fiber contraction and stretch engineering. It is a spher...

Claims

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Application Information

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IPC IPC(8): G01N33/68
Inventor 周坚熊晓芳
Owner WUXI ANKANG BIOLOGICAL TECH
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