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Fast detection method of benzodiazepines substances doped in medicine and health-care food

A technology for benzodiazepines and health food, which is applied in the detection field of benzodiazepines, can solve the problems of great influence on the separation effect, long processing time, expensive instruments, etc., and achieves wide application range and accurate High efficiency and strong specific effect

Active Publication Date: 2013-03-27
SHENZHEN INST FOR DRUG CONTROL SHENZHEN TESTING CENT OF MEDICAL DEVICES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantages of this method are: (1) the chromatographic resolution is low, the composition of traditional Chinese medicine preparations is complex, and there are many interference factors, it is easy to misjudge some coexisting components as benzodiazepines. If the migration value is close to the component to be measured, it will interfere with the detection of the target component
(2) The time for chromatographic development and drying is long, which is not enough to meet the requirements of rapid determination
(3) Thin-layer chromatography plates need to be dried and stored in a dry place, and the ambient temperature and humidity have a great influence on the separation effect
(4) A fixed place is required, and it is not suitable for on-site testing with high mobility
The disadvantages are: (1) the instrument is expensive, the sample pretreatment time is long, the chromatographic column is easily polluted, and the analysis cost is high; , Oxazepam, Estazolam and Chlordiazepoxide when the chromatographic peak retention time is close, it is easy to make a wrong judgment; (3) The instrument has high requirements for the use environment and needs to be placed fixedly, so it is not suitable for the scene with large fluidity detection
These tests can only be carried out in a small number of laboratories and are not easy to promote and use
[0016] At present, there is no rapid test method for benzodiazepines released in the pharmaceutical and health food industry. For pharmacies and grassroots drug inspection units, a rapid screening method for unauthorized addition of chemical ingredients in Chinese patent medicines and health food has been developed, and the samples are tested at the supervision site. Rapid analysis is necessary to provide timely evidence for drug supervision, crack down on counterfeiting by criminals, and ensure the safety of medication for the people

Method used

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  • Fast detection method of benzodiazepines substances doped in medicine and health-care food
  • Fast detection method of benzodiazepines substances doped in medicine and health-care food
  • Fast detection method of benzodiazepines substances doped in medicine and health-care food

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Tianxingjian Wanan Capsules (capsules, the labeled dosage is 2 capsules each time, the test results of high performance liquid chromatography-mass spectrometry, this product contains 0.353mg of clonazepam per capsule)

[0057] Take the contents of two grains, put them in a 10ml sample tube, add 5ml of ethyl acetate, vortex for 3 minutes, filter, and collect the filtrate; the filtrate is dried with a hair dryer, and the residue is the test sample; add hydrochloric acid solution to the residue 2.5ml, vortex for 3 minutes; add 5 drops of 0.1mol / L sodium nitrite solution to the hydrochloric acid solution, vortex for 1 minute; add 0.2mol / L urea 1ml and vortex for 2 minutes; 5 drops of naphthol test solution, the solution gradually appeared a red precipitate, from which it can be judged that the sample contained illegally added benzodiazepines.

Embodiment 2

[0059] Tianxingjian Wanan Capsules (capsules, the labeled dosage is 2 capsules each time, the test results of this product contain 0.557mg of nitrazepam per capsule by high performance liquid chromatography-mass spectrometry)

[0060] Take the contents of two grains, put them in a 10ml sample tube, add 3ml of ethyl acetate, vortex for 5 minutes, filter, and collect the filtrate; the filtrate is dried with a hair dryer, and the residue is the test sample; add hydrochloric acid solution to the residue 2ml, vortex for 5 minutes; add 6 drops of 0.05mol / L sodium nitrite solution to the hydrochloric acid solution, vortex for 3 minutes; add 1.5ml of 0.1mol / L urea and vortex for 4 minutes; 5 drops of naphthol test solution, the solution gradually appeared a red precipitate, from which it can be judged that the sample contained illegally added benzodiazepines.

Embodiment 3

[0062] Baizi Jingxin Capsules (capsules, the labeled dosage is 2 capsules each time, the test results of high performance liquid chromatography-mass spectrometry, each capsule of this product contains 0.572mg of estazolam)

[0063] Take the contents of two grains, put them in a 10ml sample tube, add 4ml of ethyl acetate, vortex for 4 minutes, filter, and collect the filtrate; the filtrate is dried with a hair dryer, and the residue is the test sample; add hydrochloric acid solution to the residue 4ml, vortex for 3 minutes; add 2 drops of 0.2mol / L sodium nitrite solution dropwise to the hydrochloric acid solution, vortex for 2 minutes; add 0.3mol / L urea 0.5ml and vortex for 2 minutes; 5 drops of naphthol test solution, the solution gradually appeared a red precipitate, from which it can be judged that the sample contained illegally added benzodiazepines.

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Abstract

A method for quickly detecting benzene diazepine doped in a medicine and health care food consists of the following steps: (1) extracting benzene diazepine from a medicine and health care food; (2) concentrating the extracted liquid; (3) hydrolyzing the extracted benzene diazepine;(4) adding a diazotization agent; (5) neutralizing the interference matter; and (6) adding a detection agent.

Description

technical field [0001] The invention relates to a method for detecting benzodiazepines, in particular for detecting whether benzodiazepines are added to medicines and health foods, especially traditional Chinese medicine preparations for tranquilizing and calming the nerves and functional health foods for nourishing the mind and calming the nerves method of chemical composition. Background technique [0002] The chemical constituents of benzodiazepines are a class of sedative-hypnotic drugs, and they also have anti-anxiety, central muscle relaxation, anti-convulsant, and anti-tremor effects. The chemical components of benzodiazepines are currently commonly used nitrazepam, clonazepam, lorazepam, oxazepam, estazolam and chlordiazepoxide. Clinical pharmacology studies have shown that these drugs are significantly different from barbiturates and other sedative and hypnotic drugs in terms of sedation and hypnosis, and drug overdose generally does not cause life-threatening, so ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N31/22
CPCG01N31/22
Inventor 王铁杰肖丽和王珏付春旺钟敏闫研关潇滢
Owner SHENZHEN INST FOR DRUG CONTROL SHENZHEN TESTING CENT OF MEDICAL DEVICES
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