Quick measuring method of aminopyrine mixed in medicine and health-care foods

A technology for health food and aminopyrine, which is applied in the direction of using chemical indicators for analysis, can solve the problems of high requirements for the use environment, large-scale on-site testing, and expensive instruments, and achieves easy mobility, drug safety assurance, and on-site convenience. effect of operation

Active Publication Date: 2010-12-08
SHENZHEN INST FOR DRUG CONTROL SHENZHEN TESTING CENT OF MEDICAL DEVICES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantages of this method are: (1) the chromatographic resolution is low, the composition of traditional Chinese medicine preparations is complex, and there are many interference factors, it is easy to misjudge some components as aminopyrine. The proximity of the biring will interfere with the detection of the target component
(2) The time for chromatographic development and drying is long, which cannot meet the requirements of rapid determination
(3) Thin-layer chromatography plates need to be stored dry, and the temperature and humidity of the environment have a great influence on the separation effect
(5) A fixed place is required, and it is not suitable for on-site testing with high mobility
The disadvantages are: (1) The instrument is expensive, the sample pretreatment time is long, the chromatographic column is easily polluted, and the analysis cost is high
(3) The instrument has high requirements on the use environment and needs to be placed fixedly, so it is not suitable for on-site testing with large fluidity
These two methods have high resolution and sensitivity, but they are not suitable for rapid screening and on-site detection with high fluidity due to the expensive instruments required, complicated experimental operations, and high requirements for the environment in which the instruments are used.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] Gastrodia elata capsules (Jilin Jichun Pharmaceutical Co., Ltd., capsules, marked dosage 2 capsules / time)

[0069] Take the content of 1 capsule, add 0.01g of aminopyrine (equivalent to one-tenth of the clinical minimum effective dose), put it in a 10ml sample tube, add 5ml of water, shake to make it fully dissolved, filter, collect the filtrate, and add to the filtrate Add 5ml of ethyl acetate to the mixture, shake for 2min, and let stand to obtain the ethyl acetate layer extract, transfer the extract and dry it with a hair dryer, add 5ml of water to the residue, shake to dissolve, and obtain the test solution. Add 2 drops of ferric chloride test solution and 2 drops of potassium ferricyanide test solution to the solution, and a dark blue complex is formed immediately.

[0070] Negative control sample test: take the content of 1 capsule of the same sample, put it in a 10ml sample tube, add water to dissolve, then operate as above, filter, extract, and prepare the test ...

Embodiment 2

[0072] Rheumatic Arthritis Tablets (Shenyang Dongxin Pharmaceutical Co., Ltd., tablets, yellow coating, marked dosage 4 tablets / time)

[0073] Take 2 pieces of the sample, remove the coating, grind them in a mortar, add 0.01g of aminopyrine (equivalent to one-tenth of the clinical minimum effective dose), put them in a 10ml sample tube, add 5ml of water, shake to make it fully Dissolve, filter, collect the filtrate, add 5ml of ethyl acetate to the filtrate, shake for 2min, let stand to get the ethyl acetate layer extract, transfer the extract to dry with a hair dryer, add 5ml of water to the residue, shake to make Dissolved to obtain the test solution. Add 2 drops of ferric chloride test solution and 2 drops of potassium ferricyanide test solution to the solution, and a dark blue complex is formed immediately.

[0074] Negative control sample test: Take 2 tablets of the same sample, remove the coating, grind them in a mortar, put them in a 10ml sample tube, add water to disso...

Embodiment 3

[0076] Compound Fengshining Tablets (Guangdong Luofushan Pharmaceutical Co., Ltd., tablets, marked dosage 2 tablets / time)

[0077] Take 2 pieces of samples, grind them in a mortar, add 0.01g of aminopyrine (equivalent to one-tenth of the clinical minimum effective dose), put them in a 10ml sample tube, add 5ml of water, oscillate to fully dissolve, filter, Collect the filtrate, add 5ml of ethyl acetate to the filtrate, shake for 2min, and let stand to obtain the ethyl acetate layer extract, transfer the extract to dry with a hair dryer, add 5ml of water to the residue, shake to dissolve, and obtain the Test solution. Add 2 drops of ferric chloride test solution and 2 drops of potassium ferricyanide test solution to the solution, and a dark blue complex is formed immediately.

[0078] Negative control sample test: Take 2 pieces of the same sample, grind them in a mortar, put them in a 10ml sample tube, add water to dissolve, then operate as above, filter and extract to prepare...

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Abstract

The invention discloses a quick measuring method of aminopyrine mixed in medicine and health-care foods. The method comprises the following steps of: (1) extracting an aminopyrine ingredient in medicine and health-care foods to obtain an extracting solution; (2) extracting the extracting solution by using a solvent, separating an obtained extracting solution and concentrating to obtain residues; (3) dissolving the residues once again to obtain a test article solution; and (4) adding a detecting agent to the test article solution. The invention has rapidness, convenience, strong specificity, high precision, sensitive reaction and wide application scope and is suitable for detecting whether the aminopyrine is mixed in the medicine and the health-care foods or not on site. A quick aminopyrine detection kit can be prepared according to the invention, which can meet the requirement on the supervision and the inspection of the medicine and the health-care foods in wide rural areas and middle-sized and small cities with lagged inspection technical conditions.

Description

technical field [0001] The invention relates to a method for detecting aminopyrine components, in particular to a method for detecting whether aminopyrine components are added in medicines and health foods, especially in antirheumatic traditional Chinese medicine preparations and health foods. Background technique [0002] Aminophenazone, also known as piramidong, dimethylaminoantipyrine, its chemical name is 4-dimethylamino-1,5-dimethyl-2-phenyl-3-pyrazoline Ketones with the molecular formula C 13 h 17 N 3 O. [0003] Aminopyrine has strong antipyretic and analgesic effect, which is slow and long-lasting, and its anti-inflammatory and anti-rheumatic effect is similar to that of aspirin. By inhibiting the synthesis and release of hypothalamic prostaglandins, it restores the reactivity of sensory neurons in the thermoregulatory center and plays an antipyretic effect; by inhibiting cyclooxygenase, it selectively inhibits the synthesis of prostaglandins in the hypothalamic ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N31/22
Inventor 李军殷果周寿林宋曼铜王铁杰肖丽和王珏石兴红
Owner SHENZHEN INST FOR DRUG CONTROL SHENZHEN TESTING CENT OF MEDICAL DEVICES
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