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Staphylococcus pasteuri and application thereof in decolorization

A technology of staphylococcus pastoris and staphylococcus, applied in bacteria, microorganism-based methods, textile industry wastewater treatment, etc., can solve the problems of weak environmental adaptability, dye decolorization, poor broad spectrum of single decolorizing bacteria, etc. The effect of improving efficiency

Inactive Publication Date: 2010-11-10
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] 1. Most single decolorizing bacteria have poor broad-spectrum and can only act on a few dye molecules with simple structures;
[0005] 2. Weak adaptability to the environment
Dye wastewater is usually alkaline, and the pH sometimes reaches 10-12. Most decolorizing strains cannot grow well and effectively decolorize dyes in this pH range.
[0006] Therefore, obtaining a high-efficiency strain with certain alkali resistance and azo dye decolorization ability is the key to solve the poor biological treatment effect of dye wastewater

Method used

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  • Staphylococcus pasteuri and application thereof in decolorization
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  • Staphylococcus pasteuri and application thereof in decolorization

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1. Isolation, purification and identification of Staphylococcus pasteuri Y1 CGMCC No.3431.

[0026] The screening process of Staphylococcus pasteuri (Staphylococcus pasteuri) provided by the invention is obtained from the activated sludge separation and purification of a printing and dyeing factory in Zhejiang, and the specific screening steps are as follows:

[0027] Extract sludge from a wastewater pool of a printing and dyeing factory in Zhejiang, weigh 1g, break it up with glass beads, mix well with 100ml of sterile water, and take 3-4ml of the liquid into a sterilized selective culture with a pH value of 12 culture medium at 37°C and 170 rpm for 12 hours, and the enriched samples were prepared for 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 、10 -6 、10 -7 There are 7 dilutions in total, each dilution draws 0.2ml and spreads it on the dye medium plate and cultures it at 37°C for 24 hours. strain. Inoculate the picked decolorized strains in liquid LB medium cont...

Embodiment 2

[0034] Example 2, Verification of the Decolorization Effect of Staphylococcus pasteuri Y1 CGMCC No.3431 on Acid Scarlet GR

[0035] Inoculate the LB culture medium from the slant where Staphylococcus pasteuri (Staphylococcus pasteuri) Y1 CGMCC No.3431 was preserved, activate the bacteria on a shaking table at 37°C and 150 rpm for 12-16 hours, and cultivate the bacteria to the logarithmic phase. Inoculate the activated bacterium solution into the dye medium with an inoculation amount of 5% (v / v), and place it for anaerobic culture at 37°C (anaerobic conditions are to fill the serum bottle with the bacteria for 5 minutes, and put the Air out), pH 6.8, culture time 30h-3d. After the cultivation is over, take a certain amount of culture solution and add it to the centrifuge tube, centrifuge at 13000rpm for 10min, take the supernatant and measure the absorbance value of Acid Scarlet GR at the maximum absorption wavelength of the dye at 510nm on a spectrophotometer, and use the valu...

Embodiment 3

[0043] Example 3, decolorization of acid scarlet GR by Staphylococcus pasteuri Y1 CGMCCNo.3431 under high pH conditions

[0044] According to the method for Example 2, the activated Staphylococcus pasteuri (Staphylococcus pasteuri) Y1CGMCC No.3431 bacterium liquid was inoculated into the acid scarlet GR (50mg / L) dye medium of pH=11 and pH=12 respectively, 37 ℃ static anaerobic culture for 40h. Detect and calculate decolorization rate according to the method for embodiment 2.

[0045] Acid scarlet GR (50mg / L) dye medium with pH=11 or pH=12: LB culture medium added with 50mg / L acid scarlet GR (first adjust the medium to pH 11 or 12 with NaOH, then add acidic scarlet GR Big Red GR).

[0046] The experimental results showed that the decolorization rate of the strain to Acid Scarlet GR was 91% at pH=11, and 92% at pH=12.

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Abstract

The invention discloses staphylococcus pasteuri and application thereof in decolorization. The strain is staphylococcus pasteuri CGMCC No.3431. The staphylococcus pasteuri has broad-spectrum azo dye decolorizing capacity, can adapt to extreme environment and can still decolorize azo dye under the alkaline condition. The strain has the advantages of solving the problems existing in the prior art, improving the efficiency of the conventional wastewater treatment method and having industrial application prospect.

Description

technical field [0001] The present invention relates to a strain of Staphylococcus Pasteur and its application in decolorization, in particular to a strain of Staphylococcus Pasteur and its application in removing azo dyes in production, sewage and other systems. Background technique [0002] With the development of the chemical industry, various dyes are widely used in industries such as textiles, printing and dyeing, papermaking and printing. The annual production of dyes in the world is about 10 9 kg, about 10% to 15% of dyes are released into the environment during use, causing serious pollution, of which 70% are azo dyes. Synthetic azo dyes contain azo bonds and complex aromatic ring structures, have high chemical stability and low biodegradability, and most dyes and their metabolic intermediates are mutagenic, carcinogenic and other toxic, and can be It exists in the environment for a long time and is extremely harmful to human health. Therefore, the treatment of was...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/28C02F3/34C12R1/44C02F103/30C02F103/36
Inventor 陈必强张胜琴杨光曹竹安
Owner TSINGHUA UNIV
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