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Preparation method of SPIO.SiO2-WGA (Wheat Germ Agglutinin) intestinal wall targeting contrast agent

A technology targeting contrast agent and intestinal wall, applied in the direction of nuclear magnetic resonance/magnetic resonance imaging contrast agent, etc., can solve the problems of poor adsorption of intestinal wall and poor imaging effect, so as to improve the effect of magnetic resonance imaging and MRI The effect of detection signal contrast and good biocompatibility

Inactive Publication Date: 2012-05-23
SHANGHAI NAT ENG RES CENT FORNANOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The object of the present invention is to provide a kind of WGA-wrapped SPIOSiO in view of the disadvantages of magnetic resonance contrast agents in the prior art, such as poor adsorption to the intestinal wall and poor imaging effect. 2 Preparation method of magnetic resonance nano contrast agent

Method used

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  • Preparation method of SPIO.SiO2-WGA (Wheat Germ Agglutinin) intestinal wall targeting contrast agent
  • Preparation method of SPIO.SiO2-WGA (Wheat Germ Agglutinin) intestinal wall targeting contrast agent
  • Preparation method of SPIO.SiO2-WGA (Wheat Germ Agglutinin) intestinal wall targeting contrast agent

Examples

Experimental program
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Embodiment 1

[0021] Embodiment 1: concrete steps are as follows:

[0022] 1. Prepare an aqueous solution of CTAB with a mass percentage of 3%, place it in a 100ml three-neck flask for mechanical stirring, take 2ml of SPIO chloroform solution and add it to the CTAB solution and stir mechanically for 30min. The product was placed in a 100ml beaker and stirred at 70°C for 15 minutes to obtain the SPIOCTAB solution, which was cooled for later use.

[0023]2. Add 37ml of deionized water, 3ml of SPIOCTAB solution, and 0.3ml of 25% ammonia water into a 50ml Erlenmeyer flask, and heat to 60 degrees with magnetic stirring. Dilute 0.30ml TEOS with ethanol to 1ml, and slowly add it into the reaction solution at a rate of 2ml / h using a microflow pump. After 1 hour, slowly add 0.1ml aminopropyltriethoxysilane (APTES) (20μl / min) , stop after another 4 hours of reaction. Adjust the pH of the reaction solution to 5-7, centrifuge at 13000r / min for 10min, disperse the precipitate with PBS solution with pH...

Embodiment 2

[0025] Embodiment 2: concrete steps are as follows:

[0026] 1. Prepare an aqueous solution of CTAB with a mass percentage of 4%, place it in a 100ml three-neck flask for mechanical stirring, take 2ml of SPIO chloroform solution and add it to the CTAB solution and stir mechanically for 30min. The product was placed in a 100ml beaker and stirred at 70°C for 15 minutes to obtain a SPIOCTAB solution, which was cooled for subsequent use.

[0027] 2. Add 37ml of deionized water, 3ml of SPIOCTAB solution, and 0.3ml of 25% ammonia water into a 50ml Erlenmeyer flask, and heat to 60 degrees with magnetic stirring. Dilute 0.40ml TEOS with ethanol to 1ml, and slowly add it into the reaction solution at a rate of 1ml / h using a microflow pump. After 1 hour, slowly add 0.1ml aminopropyltriethoxysilane (APTES) (20μl / min ), and stopped after another 4 hours of reaction. Adjust the pH of the reaction solution to 5-7, centrifuge at 13000r / min for 10min, disperse the precipitate with PBS solut...

Embodiment 3

[0029] Embodiment 3: concrete steps are as follows:

[0030] 1. Prepare a CTAB aqueous solution with a mass percentage of 3.6%, place it in a 100ml three-necked flask and stir it mechanically, take 2ml SPIO chloroform solution and add it to the CTAB solution and stir it mechanically for 30min. The product was placed in a 100ml beaker and stirred at 70°C for 15 minutes to obtain a SPIOCTAB solution, which was cooled for subsequent use.

[0031] 2. Add 37ml of deionized water, 3ml of SPIOCTAB solution, and 0.3ml of 25% ammonia water into a 50ml Erlenmeyer flask, and heat to 60 degrees with magnetic stirring. Dilute 0.36ml of TEOS with ethanol to 1ml, and slowly add it into the reaction solution at a rate of 1.5ml / h using a microflow pump. After 1 hour, slowly add 0.1ml of aminopropyltriethoxysilane (APTES) (20μl / min), and stopped after another 4 hours of reaction. Adjust the pH of the reaction solution to 5-7, centrifuge at 13000r / min for 10min, disperse the precipitate with ...

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Abstract

The invention relates to a preparation method of a wheat germ agglutinin (WGA) packaging silica superparamagnetism (SPIO@SiO2-WGA) magnetic resonance nanometer contrast agent for biomedicine diagnosis. In the invention, water solubility conversion of oil soluble SPIO is realized by utilizing hexadecyl trimethyl ammonium bromide; silica packaging and surface amination of the SPIO are realized by adopting ethyl orthosilicate and aminopropyltriethoxysilane; and connection between the wheat germ agglutinin which has adsorption on the intestinal wall and a superparamagnetism silica sphere is realized by utilizing EDC / NHS in PBS solution. The obtained SPIO.SiO2-WGA magnetic resonance nanometer contrast agent has the advantages of uniform and stable particle diameters, low toxicity and obvious cell targeting effect.

Description

technical field [0001] The invention relates to a preparation method of a superparamagnetic iron tetroxide (SPIO: superparamagnetic iron oxide) magnetic resonance nano-contrast agent, in particular to a SPIOSiO 2 - Preparation method of WGA intestinal wall targeting contrast agent. Background technique [0002] Magnetic resonance imaging plays a very important role in the diagnosis of tumor diseases and is widely used in clinical detection. On the one hand, its imaging effect largely depends on the contrast agent used. A good contrast agent or a contrast agent with specific targeting can improve the contrast of diagnostic signals, so that tumors can be detected earlier. According to the current medical level, about 80% to 90% of early cancer patients can be cured. Therefore, the development of magnetic resonance contrast agents with excellent performance, so as to detect tumors early, is the key to the treatment of malignant tumors. [0003] With the continuous developmen...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K49/06
Inventor 陈高祥程学军陆庆王平翠刘睿许建荣金彩虹何丹农
Owner SHANGHAI NAT ENG RES CENT FORNANOTECH
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