Tissue-culture seedling raising method of rhodiola crenulata
A technology of Rhodiola grandiflora and single seedlings, applied in horticultural methods, botanical equipment and methods, cultivation, etc., can solve problems such as inability to carry out large-scale planting and production, long subculture and multiplication cycle, and inability to realize large-scale production, and achieve a solution Difficult rooting, early start, and improved germination rate
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Embodiment 1
[0041] The concrete steps of the group cultivation seedling method of Rhodiola grandiflora in the present embodiment are as follows:
[0042] (1) Select the leaves of Rhodiola grandiflora excellent single plant (collected from perennial wild plants in Tibet) as explants. When disinfecting the explants, wash them with tap water for 30 minutes, and then wash them in a sterile ultra-clean bench. Disinfect with 10% 84 disinfectant for 25 minutes, and then wash with sterile water for 3 times.
[0043] (2) Sterilized explants were induced to sprout under sterile conditions, the basic medium was MS medium, and the additional plant hormones were 15 μmol / L of 6-BA and 2.5 μmol / L of GA 3 , also contains 5.5g / L of agar and 26g / L of sucrose, and the pH of the medium is 5.8. The explants were treated in the dark for 10 days on the germination induction medium, and then cultured under the condition of light intensity of 2500±500Lux, the culture temperature of the induced adventitious buds wa...
Embodiment 2
[0048] The concrete steps of the group cultivation seedling method of Rhodiola grandiflora in the present embodiment are as follows:
[0049] (1) Select the leaves of Rhodiola daflora excellent individual plants as explants. When disinfecting the explants, wash them with tap water for 30 minutes, and then disinfect them with 10% 84 disinfectant in a sterile ultra-clean bench. 25 minutes, and then washed 3 times with sterile water.
[0050] (2) Sterilized explants were induced to sprout under sterile conditions, the basic medium was MS medium, and the additional plant hormones were 15 μmol / L of 6-BA and 2.5 μmol / L of GA 3 , also contains 6g / L of agar and 20g / L of sucrose. The explants were treated in the dark on the germination induction medium for 5 days, and then cultivated under the condition of light intensity of 2500±500Lux, the culture temperature of induced adventitious buds was 25±2°C, the pH of the medium was 5.8, and the light time was 16 hours, 12 Days differentiat...
Embodiment 3
[0055] The concrete steps of the group cultivation seedling method of Rhodiola grandiflora in the present embodiment are as follows:
[0056] (1) Select the leaves of Rhodiola daflora excellent individual plants as explants. When disinfecting the explants, wash them with tap water for 30 minutes, and then disinfect them with 10% 84 disinfectant in a sterile ultra-clean bench. 25 minutes, and then washed 3 times with sterile water.
[0057] (2) Sterilized explants were induced to sprout under sterile conditions, the basic medium was MS medium, and the additional plant hormones were 15 μmol / L of 6-BA and 2.5 μmol / L of GA 3 , also contains 6.5g / L of agar and 30g / L of sucrose. The explants were treated in the dark for 0 days on the germination induction medium, and then cultured under the condition of light intensity of 2500±500Lux, the culture temperature of induced adventitious buds was 25±2°C, the pH of the medium was 5.8, and the light time was 16 hours, Days differentiated....
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Abstract
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