Lymph gland targeted metastatic human hepatoma cell strain and establishing method thereof

A human liver cancer cell line and a technology for liver cancer cells, which are applied in the field of human liver cancer cell line HCCLM3-R-LnM1 and its establishment, can solve problems such as no human liver cancer cell lines, and achieve delayed fluorescence quenching time and strong transfer ability. , the effect of genetic stability

Inactive Publication Date: 2010-09-15
ZHONGSHAN HOSPITAL FUDAN UNIV
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Problems solved by technology

There are no reports on lymph node-targeted metastatic human liver cancer cell lines that can stably express fluorescence in domestic and foreign literatures

Method used

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  • Lymph gland targeted metastatic human hepatoma cell strain and establishing method thereof
  • Lymph gland targeted metastatic human hepatoma cell strain and establishing method thereof
  • Lymph gland targeted metastatic human hepatoma cell strain and establishing method thereof

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Embodiment 1

[0023] 1. Establishment of HCCLM3R nude mouse subcutaneous xenograft tumor model: BALB / c nu / nu male nude mice, 5-6 weeks old, weighing 15-18 grams, were raised in the nude mouse room of our institute without specific pathogens. Inject HCCLM3R, a highly metastatic fluorescent cell of liver cancer cultured in vitro, subcutaneously on the left side of the back, at a dose of 2X10 7 Cells / only, subcutaneous tumors were aseptically harvested after 4 weeks of growth, cut into 1mm 3 Left and right tumor small pieces for follow-up experiments;

[0024] 2. Establish HCCLM3R nude mouse liver transplantation tumor model: locate the nude mouse liver, disinfect the skin outside the liver, incise the skin aseptically to expose the mouse liver, separate the liver capsule with tweezers and make a small opening, and implant the tumor piece into the liver On the surface, the liver capsule and skin were sutured.

[0025] 3. Acquisition of lymph node metastatic liver cancer cells: the orthotopic...

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Abstract

The invention provides a lymph gland targeted metastatic human hepatoma cell strain. The mother cell is a red fluorescent protein-expressed hepatoma cell HCCLM3-R. The human hepatoma cell strain is characterized in that the cell is named as lymph gland targeted metastatic human hepatoma cell strain HCCLM3-R-LnM1 according to the classified nomenclature with a preservation number of 3645, and has the following biological characteristics: the cell is polygonal epithelioid and is in adherent growth and loss of contact growth inhibition, is of a hypo-triploid karyotype, and has 50-58 of chromosomes each of which secretes AFP proteins. The establishing method comprises the following steps: using subcutaneous and liver orthotopic transplantation tumor models of a nude mouse to carry out spontaneous lymphatic metastasis screening, and then carrying out in-vitro cloning, amplification and purification on the lymphatic metastasis human hepatoma cell. The invention has wide application prospects and potential social and economic values in the basic research on hepatoma and the pre-clinic research on medicament.

Description

technical field [0001] The present invention relates to a lymph node-targeted metastatic human liver cancer cell line and its establishment method, in particular to a human liver cancer cell line HCCLM3-R-LnM1 capable of stably expressing red fluorescent protein (RFP) and capable of lymph node-targeted metastasis and its establishment method The invention belongs to the technical field of microbial animal cell lines. Background technique [0002] Primary hepatocellular carcinoma (HCC) is one of the most common malignant tumors in my country and even in the world, and its morbidity and mortality rate ranks second among malignant tumors. There are many methods to treat liver cancer clinically, such as surgical resection, TACE, intratumoral alcohol injection, radiofrequency therapy and radiotherapy, etc., but the long-term efficacy is limited. According to the long-term clinical follow-up data of more than 9,800 liver cancer patients operated by Fudan University Liver Cancer I...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/09C12R1/91
Inventor 吴伟忠陶中华李薇薇孙惠川汤钊猷樊嘉钦伦秀王鲁
Owner ZHONGSHAN HOSPITAL FUDAN UNIV
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