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Large green frog antioxidant peptide (antioxidin-RL) as well as gene and application thereof

An anti-oxidation peptide and gene technology, applied in the field of biomedicine, can solve the problem that the active ingredients of amphibians cannot play a good role, and achieve the effect of convenient artificial synthesis, simple structure and strong anti-inflammatory activity

Inactive Publication Date: 2010-09-08
KUNMING INST OF ZOOLOGY CHINESE ACAD OF SCI
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Problems solved by technology

Due to the fact that the whole body is often used as medicine, coupled with the limitations of its traditional processing methods, amphibians as traditional Chinese medicinal materials often cannot play a good role in their active ingredients. It is the traditional Chinese medicine to find specific active monomer compounds from these traditional medicines. One of the important contents of modern

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  • Large green frog antioxidant peptide (antioxidin-RL) as well as gene and application thereof

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Embodiment Construction

[0028] The substantive content of the present invention is further described below with embodiment, but content of the present invention is not limited thereto.

[0029] Clone of the green frog antioxidant peptide antioxidant-RL gene:

[0030] I. Extraction of total RNA from the skin of the green frog:

[0031] A. The live big green frog was cleaned with water, put into liquid nitrogen and quick-frozen for 4 hours, and the skin tissue was taken and weighed.

[0032] Take 300 mg of skin tissue, add 10 ml of total RNA extraction buffer (Trizol solution, product of GIBCOBRL, USA), and homogenize in a 20 ml glass homogenizer for 30 minutes.

[0033] B. Add an equal volume of phenol / chloroform solution, vortex and mix well, let stand at room temperature for 10 minutes, centrifuge at 12000 rpm for 10 minutes at 4°C, and discard the precipitate.

[0034] C. Add an equal volume of isopropanol to the supernatant, place at room temperature for 10 minutes, centrifuge at 12,000 rpm at 4...

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Abstract

The invention relates to a large green frog antioxidant peptide (antioxidin-RL) as well as a gene and application thereof, belonging to the technical field of biomedicine. The large green frog antioxidant peptide (antioxidin-RL) is a single-chain polypeptide coded by the gene of the large green frog antioxidant peptide (antioxidin-RL) and has the molecular weight of 1672.98 Daltons and an isoelectric point of 9.31, and the complete sequence of the large green frog antioxidant peptide (antioxidin-RL) is alanine-methionine-arginine-leucine-threonine-tyrosine-asparagine-arginine-proline-cysteine-isoleucine-tyrosine-alanine-threonine. The coded genes consist of 306 nucleotides, wherein a mature peptide part is coded by 130-171 site nucleotides. The artificially synthesized large green frog antioxidant peptide (antioxidin-RL) has outstanding antioxidant activity, can be applied to preparing skin antioxidant protecting and in-vivo radicals scavenging drugs and also has simple sequence and convenient synthesization.

Description

Technical field: [0001] The invention provides a green frog (Rana livida) antioxidant peptide antioxidant-RL and its gene and application, belonging to the technical field of biomedicine. Background technique: [0002] In traditional Chinese medicine and ethnic medicine in China, many amphibians are widely used as medicinal materials, such as Bufo bufo, Bufo tibetanus, Hyla annectans, Rana boulengeri, Spot-legged pan-tree frog (Polypedates leucomystax), and flower princess frog (Microhyla pulchra). Its reported pharmacological activities and clinical efficacy include anti-inflammation, anti-tumor, local anesthesia, analgesia, immune regulation and cardiovascular regulation. Due to the fact that the whole body is often used as medicine, coupled with the limitations of its traditional processing methods, amphibians as traditional Chinese medicinal materials often cannot play a good role in their active ingredients. It is the traditional Chinese medicine to find specific activ...

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Application Information

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IPC IPC(8): C07K7/08C12N15/12A61K38/10A61K8/64A61P39/06A61Q19/08
Inventor 赖仞刘存宝杨海龙李东升
Owner KUNMING INST OF ZOOLOGY CHINESE ACAD OF SCI
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