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Method for preparing polyaniline-alginic acid-ionic liquid-enzyme composite membrane modified electrode

Inactive Publication Date: 2010-08-04
WUHAN INSTITUTE OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the preparation process of polyaniline-enzyme modified electrodes in the prior art is generally complicated and the preparation cycle is long

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] 1) dissolving aniline and alginic acid in a hydrochloric acid solution with a pH of 1 to obtain solution A, the concentration of aniline in solution A is 0.01mol / L, and the mass percent concentration of alginic acid is 0.1%;

[0020] 2) Add ionic liquid 1-butyl-3-methylimidazolium phosphorus hexafluoride to solution A, and stir for 1 hour to obtain solution B, and the mass of the added ionic liquid is 2% of solution A;

[0021] 3) Add peroxidase to solution B, sonicate for 30 minutes to obtain solution C, the concentration of peroxidase in solution C is 0.01mg / mL;

[0022] 4) Electrochemical cyclic voltammetry synthesis was carried out in solution C equipped with a gold working electrode, a counter electrode and a reference electrode, the cycle potential was between -0.25-0.85V, and the number of scanning cycles was 20 circles to obtain polyaniline- Alginate-ionic liquid-enzyme composite membrane modified electrode.

Embodiment 2

[0024] 1) dissolving aniline and alginic acid in a hydrochloric acid solution with a pH of 2 to obtain solution A, the concentration of aniline in solution A is 0.03mol / L, and the mass percent concentration of alginic acid is 0.2%;

[0025] 2) Add ionic liquid 1-butyl-3-methylimidazolium phosphorus hexafluoride to solution A, and stir for 2 hours to obtain solution B, and the mass of the added ionic liquid is 1% of solution A;

[0026] 3) Add glucose oxidase to solution B, sonicate for 20 minutes to obtain solution C, the concentration of glucose oxidase in solution C is 1 mg / mL;

[0027] 4) Electrochemical cyclic voltammetry synthesis was carried out in solution C equipped with a platinum working electrode, a counter electrode and a reference electrode, the cycle potential was between -0.25-0.85V, and the number of scanning cycles was 100 cycles to obtain polyaniline- Alginate-ionic liquid-enzyme composite membrane modified electrode.

Embodiment 3

[0029] 1) dissolving aniline and alginic acid in a hydrochloric acid solution with a pH of 3 to obtain solution A, the concentration of aniline in solution A is 0.05mol / L, and the mass percent concentration of alginic acid is 0.3%;

[0030] 2) Add ionic liquid 1-butyl-3-methylimidazolium boron tetrafluoride to solution A, and stir for 1 hour to obtain solution B, and the quality of the added ionic liquid is 2% of solution A;

[0031] 3) Add lactate oxidase to solution B, sonicate for 10 minutes to obtain solution C, the concentration of lactate oxidase in solution C is 0.5 mg / mL;

[0032] 4) Electrochemical cyclic voltammetry synthesis was carried out in solution C equipped with indium tin oxide working electrode, counter electrode and reference electrode. Aniline-alginic acid-ionic liquid-enzyme composite membrane modified electrode.

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PUM

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Abstract

The invention relates to a method for preparing a polyaniline-alginic acid-ionic liquid-enzyme composite membrane modified electrode, which comprises the following steps of: 1) dissolving aniline and alginic acid in solution of hydrochloric acid to obtain solution A; 2) adding ionic liquid into the solution A, and stirring the mixture to obtain solution B; 3) adding an enzyme into the solution B,and performing ultrasonic processing on the mixture to obtain solution C; and 4) and performing electrochemical cyclic voltammetry synthesis in the solution C for preparing a working electrode, a counter electrode and a reference electrode to obtain the polyaniline-alginic acid-ionic liquid-enzyme composite membrane modified electrode. Compared with the prior art, the method achieves the technical effects of simple reaction condition, easy operation, short preparation cycle and controllable composite membrane thickness.

Description

technical field [0001] The invention relates to a preparation method of a polyaniline-alginic acid-ionic liquid-enzyme composite membrane modified electrode. Background technique [0002] The special structure and excellent physical and chemical properties of conductive polymers make them have broad and attractive application prospects in biosensors, electromagnetic shielding, metal anticorrosion and stealth technology. Among various conductive polymers, polyaniline has the characteristics of easy film formation, good film stability, and easy chemical or electrochemical synthesis. It is one of the most studied systems in the field of conductive polymers. Polyaniline-modified enzyme electrodes are a hotspot in the research of biosensors, and a variety of preparation methods have been developed. The choice of enzyme immobilization method is crucial for the preparation of sensors with fast response, high sensitivity, good stability and long service life. Alginic acid has the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327
Inventor 李亮魏玉研潘杰鄢国平喻湘华
Owner WUHAN INSTITUTE OF TECHNOLOGY
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