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Method for purifying gonadotropins

A technology of gonadotropin and purification method, which is applied in the field of gonadotropin purification, can solve the problem of low yield, and achieve the effect of high yield and high titer

Active Publication Date: 2010-08-04
上海丽珠生物科技有限公司
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AI Technical Summary

Problems solved by technology

The current commonly used purification methods in China have a low yield (generally less than 50%), and the potency of the finished product is around 100iu / mg

Method used

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  • Method for purifying gonadotropins
  • Method for purifying gonadotropins

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Experimental program
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Embodiment

[0018] 1. Process operation

[0019] The purification process flow chart used in the embodiment is as follows figure 1 As shown, the specific operation method is as follows:

[0020] 1 Dissolution, adsorption, washing, elution, precipitation, drying:

[0021] 1.1 Dissolution:

[0022] Accurately weigh 300g of crude urinary gonadotropin on a 1kg tray balance, put it in a 40L stainless steel bucket, add 30L of 0-15℃ solution A, place it on an explosion-proof mixer, stir with a stainless steel stirring rod at a speed of 200-400 rpm / Min, control the temperature at 0-15℃ and stir to dissolve.

[0023] 1.2 Adsorption:

[0024] Under stirring (rotating speed 200-400 rpm), slowly add 3 kg of aluminum silicate powder to the above-mentioned dissolving solution, continue stirring for 2 hours, control the temperature at 0-15°C, obtain the adsorption solution, and move it into the centrifuge room.

[0025] 1.3 Washing:

[0026] Pour the above-mentioned adsorption solution into a clean plastic wide-mo...

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Abstract

The invention provides a method for purifying gonadotropins extracted from urine of postmenopausal women. Compared with the extraction separation of the existing gonadotropins, the method of the invention has the following advantage: the gonadotropins which are obtained by the method of the invention and take FSH and LH as the main components have higher titer which is more than 150iu / mg) and higher yield which is more than 75%.

Description

Technical field [0001] The invention relates to a method for purifying gonadotropin, in particular to a method for purifying gonadotropin extracted from urine of menopausal women. Background technique [0002] Gonadotropin (gonadotropic hormone) refers to a type of hormone that can promote the development of male and female gonads and increase the secretion of sex hormones, such as luteinizing hormone (LH) and follicle stimulating hormone (FSH) secreted by the anterior pituitary gland. Chorionic Gonadotropin (HCG) secreted by the placental chorion. Among them, the main role of FSH and LH is to promote the production and secretion of steroid hormones in the ovary or testis. They can be used in combination with HCG to treat primary or secondary amenorrhea caused by insufficient gonadal hormone secretion, and anovulatory sparse menstruation. Caused by infertility. Due to the lack of estrogen and progesterone in menopausal women, they feedback and regulate the pituitary gland, secr...

Claims

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Application Information

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IPC IPC(8): C07K1/36C07K1/30C07K1/18C07K14/59
Inventor 万龙岩周金
Owner 上海丽珠生物科技有限公司
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