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Graft polymerization method of vegetable protein and polysaccharide

A plant protein and graft polymerization technology, which is applied in the field of plant protein functional characteristics, can solve the problems of short reaction time, yellowish color of reaction products, and difficulty in industrial production, etc., to achieve increased reaction speed, small appearance color, and acceptability Good results

Inactive Publication Date: 2010-07-28
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the speed of the wet heat grafting reaction has increased, it is currently mainly used in the reaction between simple proteins and monosaccharides or disaccharides, and has a significant impact on the color of the product
Therefore, these two methods all have technical bottlenecks such as long reaction time and darker color of the reaction product, and are difficult to be applied to industrial production
[0005] Guan Jun et al. found that the graft copolymer of protein and sugar can be obtained by microwave heating for 10-60 min, which speeds up the grafting reaction rate of protein and sugar; Wang Jinshui et al. , 20-30 minutes of intermittent ultrasonic treatment can promote the graft copolymerization of protein and polysaccharide. The reaction time of the two methods is short, but the reaction product still has technical problems such as yellowish color and continuous industrial production.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Dissolve 100g of soybean protein isolate in 1L of water, add 100g of gum arabic, stir for 3 hours, add sodium hydroxide to adjust the pH value to 7.5, and treat it with a pulse electric field with an intensity of 15KV. The pulse treatment time is 1300μs and the frequency is 1000Hz. Insoluble material was removed by centrifugation, and the supernatant was freeze-dried. A grafted mixture of soybean protein and gum arabic was obtained.

Embodiment 2

[0028] Dissolve 100g of soybean protein isolate in 1L of water, add 30g of dextrose, stir for 2 hours, add sodium hydroxide to adjust the pH value to 7.0, and treat it with a pulsed electric field with an intensity of 30KV. The pulse treatment time is 480μs and the frequency is 1500Hz. Liquid freeze-dried. A soybean protein isolate and dextrose grafted mixture is obtained.

Embodiment 3

[0034] Dissolve 100g of soybean protein isolate in 1L of water, stir for 3 hours, add sodium hydroxide to adjust the pH value to 8, add 30g of dextrin, and process it through a pulsed electric field with an intensity of 40KV. The pulse treatment time is 780μs and the frequency is 2000 Hz. Concentrate and then spray dry to obtain the grafted soybean protein isolate and dextrin mixture.

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PUM

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Abstract

The invention discloses a graft polymerization method of vegetable protein and polysaccharide, which includes that: (1) the graft polymerization of vegetable protein and polysaccharide: the vegetable protein is dissolved and is mixed with polysaccharide to obtain mixture solution, the mass ratio of vegetable protein and polysaccharide is 0.1-10:1; (2) the mixture solution is stirred for 2-3 hours, and then the mixture solution is treated by the pulse electric field with frequency of 500-2000 Hz and voltage of 15-45KV for 480-2000us to obtain copolymer; (3) the copolymer is concentrated and dried through atomizing. The invention has high reaction efficiency, short reaction time, stable performance, no pollution and also greatly improves the solubility and emulsifying of the copolymer; meanwhile, the equipment can be used for continuous industrial production.

Description

technical field [0001] The invention belongs to the technical field of plant protein functional characteristics, and in particular relates to a pulse electric field-promoted graft polymerization method of plant protein and polysaccharide. Background technique [0002] The basic characteristics of protein in food and food base materials can be summarized into two aspects: nutritional characteristics and functional characteristics. Nutritional characteristics have no direct impact on the acceptability of food, but are closely related to the nutrition of the body that ingests food. Plant protein has a variety of functional properties, but it is difficult to have multiple processing functions at the same time. What is required in production is a product with an optimal function or a balance of several functions. How to apply it to various The core of different types of food systems is modification. [0003] Since the 1980s, the graft modification of proteins and polysaccharides...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J3/14
Inventor 赵谋明崔春穆丽霞赵强忠
Owner SOUTH CHINA UNIV OF TECH
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