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Cycling elution biomimetic peristaltic enzymolysis reactor and method thereof

An enzymatic hydrolysis reactor and leaching technology, applied in the direction of enzyme production/bioreactor, bioreactor/fermenter combination, specific-purpose bioreactor/fermenter, etc., can solve the difficulty of enzyme recovery, the charging coefficient The problems of low sugar concentration and low sugar concentration can achieve the effect of simple reactor structure, increased contact surface and low energy consumption.

Inactive Publication Date: 2013-11-06
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] In order to overcome the deficiencies of existing enzymolysis reactors such as low loading coefficient, difficulty in enzyme recovery, low sugar concentration, substrate inhibition, etc., the purpose of the present invention is to provide an integrated cellulase cycle leaching, bionic enzymolysis and reducing sugar Development of an integrated biomimetic membrane separation enzymolysis reactor with cyclic elution for membrane circulation separation

Method used

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  • Cycling elution biomimetic peristaltic enzymolysis reactor and method thereof
  • Cycling elution biomimetic peristaltic enzymolysis reactor and method thereof
  • Cycling elution biomimetic peristaltic enzymolysis reactor and method thereof

Examples

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Embodiment 1

[0038] A bionic peristaltic enzymolysis reactor for cyclic rinsing in this embodiment has a bionic peristaltic main reactor with a height-to-diameter ratio of 2, a total design volume of 50 L, and a bottom distribution diameter of the elastic inner wall of the main enzymatic hydrolysis reactor of 0.5 mm pores, the filter membrane pore size is 75μm, leaching is realized by installing a nozzle, the peristaltic rod is realized by a motor combined with a crankshaft, and the peristaltic rod on the side wall of the reactor moves in parallel in the same direction; the moving speed of the peristaltic rod is 1m / s, and the The cycle interval is 5min.

[0039] The steps of the enzymolysis operation in this reactor are as follows: After the raw material of the steam-exploded straw is sterilized, it is transported to the main reactor for peristaltic enzymolysis by the screw feeder and the distributor, and the charging coefficient of the steam-exploded straw is 85%; The diluted enzyme solut...

Embodiment 2

[0041] This example adopts the enzymolysis reactor of Example 1, and the enzymolysis operation steps are as follows: After the steam-exploded straw raw material is sterilized, it is transported to the peristaltic enzymolysis main reactor by a screw feeder and a distributor, and the steam-exploded straw is The filling factor of the enzyme solution is 90%; the diluted enzyme solution is evenly sprayed on the substrate through the nozzle according to the solid-liquid ratio (w / v, g / ml) of 1:10; Make the steam-exploded straw crawl in the reactor for enzymolysis; at the same time, open the outlet of the enzymolysis liquid at the bottom of the reactor, so that the cellulose enzyme liquid and enzymolysis liquid enter the main enzymolysis reactor through the membrane separation system, and then form a circular rinse through the nozzle ; When the sugar concentration analyzer reaches the preset value, control the pressure at both ends of the membrane module to 0.1 MPa, and add buffer at t...

Embodiment 3

[0043] A biomimetic peristaltic enzymolysis reactor for cyclic rinsing in this embodiment, the bionic peristaltic enzymolysis main reactor has a height-to-diameter ratio of 2.5, a total design volume of 100L, and a bottom distribution diameter of the elastic inner wall of the enzymolysis main reactor is 1mm The pore size of the filter membrane is 200 μm. The leaching is realized by installing a nozzle. The peristaltic rod is realized by a reciprocating piston. The reciprocating cycle time of the peristaltic rod is 20 minutes. The peristaltic rods on the side wall of the reactor move in opposite directions at intervals.

[0044] The steps of the enzymolysis operation in this reactor are as follows: After the raw material of the steam-exploded straw is sterilized, it is transported to the main reactor for peristaltic enzymolysis by the screw feeder and the distributor, and the charging coefficient of the steam-exploded straw is 80%; The diluted enzyme solution is evenly sprayed ...

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Abstract

The invention discloses a cycling elution biomimetic peristaltic enzymolysis reactor and a method thereof, comprising a biomimetic peristaltic enzymolysis reactor, a peristaltic driving device (10), an elution device (19), a membrane cycling separation device (12), enzymolysis solution cycling pump (15), a sugar concentration analyzer (20), spiral material feeding / discharging devices (8,9) and a control system. The peristaltic device comprises a peristaltic rod and a peristaltic driving device, and conducts reciprocating motion at the parallel direction of the axial direction of the reactor; the sugar concentration analyzer controls cycling elution of cellulase in the enzymolysis reactor and separation and concentration of the cellulase and the sugar solution in the membrane separation system. The characteristics of the reactor are as follows: through the biomimetic peristaltic role, mass transportation between the solid-phase substrates is improved, and the accessibility of solid-phase substrate is increased; the cycling elution enzymolysis can solve the problem of low loading coefficient, low loading coefficient for traditional reactor, the invention provides high-concentration sugar platform; due to the enzymolysis process without solid flipping mechanical transmission, the reactor with simple structure has low energy consumption, facilitates the industrial scale-up.

Description

【Technical field】 [0001] The invention belongs to the technical field of enzymatic hydrolysis and saccharification of lignocellulosic raw materials, and in particular relates to an integrated cycle rinsing bionic peristaltic enzymolysis reactor integrating cellulase cyclic leaching, peristaltic enzymatic hydrolysis and sugar film cyclic separation and a method thereof. 【Background technique】 [0002] Lignocellulosic biomass will be an important raw material for human energy, materials and chemical products in the future. However, due to its high heterogeneity and complexity, lignocellulosic raw materials have been difficult to break through the economic and technological barriers as a resource for large-scale biological utilization. One of the important reasons is that the enzymatic hydrolysis efficiency of cellulose raw materials is low and the cost of enzymatic hydrolysis is high. Therefore, it is very important to develop a new type of high-efficiency enzymatic hydrolysis...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/02
CPCC12M29/02C12M47/10C12M21/18C12M29/04
Inventor 陈洪章邱卫华
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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