Bacillus calmette-guerin polysaccharide and nucleic acid preparation for treating respiratory diseases for livestock

A BCG polysaccharide nucleic acid and BCG technology, which can be used in respiratory diseases, allergic diseases, medical raw materials derived from bacteria, etc., can solve the problem that antibiotics have no significant therapeutic effect on respiratory diseases, and traditional Chinese medicine administration methods cannot be widely used. It can not be widely used, and can not be widely promoted, so as to avoid the production of drug resistance, reduce the amount of organic reagents, and reduce human and material resources.

Inactive Publication Date: 2010-06-30
TIANJIN RINGPU BIO TECH
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  • Summary
  • Abstract
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AI Technical Summary

Problems solved by technology

Although this method has higher productive rate compared with conventional method, this method requires long time (greater than 3 hours) operating condition, not only time is long, energy consumption is many, and long time high rotating speed (greater than 10000 rpm) The operation has a certain damage effect on the high-speed shearing machine, which relatively increases the production cost, so it cannot be widely promoted in actual production
[0006] Winter and spring are the frequent periods of livestock and poultry respiratory diseases. At present, antibiotics are used as the main therapeutic drug in the treatment of livestock and poultry respiratory diseases. However, due to the existence of drug resistance, most antibiotics have no significant therapeutic effect on the treatment of respiratory diseases. Traditional Chinese medicine cannot be widely used due to limitations in dosage forms and administration methods.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Extraction of BCG polysaccharide nucleic acid: add 5kg BCG, 5kg water for injection depyrogenated by ultrafiltration, after mixing, use a high-pressure homogenizer to break the wall at 60MPa and 50Hz, add 1kg chitosan with a mass concentration of 1% As a flocculant, run continuously for 3 hours to break the wall and collect high-pressure homogenized homogenate;

[0040] Put the homogenate in a 20-liter stainless steel bucket, insert the bactericidal knife into the liquid, the knife is about 5 cm away from the bottom of the bucket, turn on the power, and run continuously at 6000 rpm until the required homogenate is collected;

[0041] The prepared homogenate is further extracted by a general extraction method to obtain the raw powder of nucleic acid and polysaccharide, and the product yield and component content are calculated.

[0042] The obtained data were compared with the extraction rates of polysaccharides and nucleic acids extracted from crushed tissues by ordinar...

Embodiment 2

[0054] Embodiment 2: take appropriate amount of BCG, equal amount of water for injection depyrogenated by ultrafiltration, after mixing, use a high-pressure homogenizer to break the wall with 60MPa, 50Hz, add 20% chitosan with a mass concentration of 1% as flocculation Run continuously for 3 hours to break the wall, collect high-pressure homogenized homogenate; put the homogenate into the stainless steel bucket, insert the bactericidal knife into the liquid, the knife is about 5 cm away from the bottom of the bucket, turn on the power, and Continuous operation at 6000 rpm until the required homogenate is collected; the prepared homogenate is further extracted and prepared by a general extraction method to obtain BCG polysaccharide nucleic acid injection, which is used to prevent and treat livestock and poultry caused by viruses or other causes For respiratory diseases, by regulating cellular immunity, humoral immunity, stimulating the reticuloendothelial system, activating the ...

Embodiment 3

[0055] Embodiment 3: the preparation of lyophilized powder: get appropriate amount of NaH 2 PO 4 .12H 2 O, Na 2 HPO 4 .2H 2 O and NaCl were dissolved in water for injection, mixed evenly and then diluted with water for injection, and the measured pH value should be 7.2±0.2; take appropriate amount of the above-mentioned buffer solution preheated to 45°C in two containers, and add them to the above-mentioned process. The obtained BCG polysaccharide and nucleic acid raw powder were stirred and dissolved, and kept in a water bath at 45°C for 30 minutes, combined the two liquids in one of the containers, rinsed the other container with a small amount of buffer solution, incorporated the washing liquid into the above solution, and heated again at 45°C. Keep warm for 40 minutes, and keep stirring gently during the heat preservation process; combine the above solutions after the heat preservation is over, use buffer to make up to 200ml, and stir evenly; add the above prepared sol...

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Abstract

The invention relates to a bacillus calmette-guerin polysaccharide and nucleic acid preparation for treating respiratory diseases for livestock. The method for preparing the preparation comprises a new process of extracting polysaccharide and nucleic acid from bacillus calmette-guerin, particularly using a high-pressure homogenizing machine to crush the bacillus calmette-guerin, and crushing the bacterium by combining the high-pressure homogenizing machine with the conventional high-speed tissue stamping technique to extract the polysaccharide and nucleic acid from the bacillus calmette-guerin. The extract can be prepared into different preparations as required, and the preparations are used for preventing and treating the respiratory disease of the livestock.

Description

technical field [0001] The invention relates to the field of veterinary biological products, in particular to a BCG polysaccharide nucleic acid preparation for livestock and poultry. Background technique [0002] BCG polysaccharide nucleic acid is a polysaccharide nucleic acid preparation extracted from BCG by thermal phenol method. It contains polysaccharide nucleic acid and other substances with immune activity. It can not only regulate cellular immunity, humoral immunity, stimulate reticuloendothelial System, activate monocyte-macrophage function, enhance natural killer cell function to enhance the body's disease resistance; it can also stabilize mast cells, block IgE function, reduce the release of active substances from degranulation cells, and have anti-acetylcholine-induced bronchial Spasm effect, to achieve anti-allergic and anti-asthma effects. Further studies have found that BCG polysaccharide nucleic acid has a two-way regulatory effect, which can promote the tra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/74A61K31/715A61P37/04A61P11/06A61P37/08
Inventor 刘菁苏建东李旭东
Owner TIANJIN RINGPU BIO TECH
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