Protein gel determining method

Abstract

The invention discloses a protein gel determining method. The method comprises the following steps of: slowly pouring weighed protein powder and cooling water with the temperature of about 5 DEG C into a stirrer to be thoroughly stirred for 1 minute; stopping stirring at the interval of 20 seconds; determining the pH value and the temperature at each stopping; completely transferring the homogenized protein into a large sample bag, and then pumping vacuum; filling the vacuumed protein into a mould, and making three parallel samples for each sample; placing the filled mould into a water bath boiler to be heated; immediately placing the heated mould into cold water to be cooled; and determining the cooled gel by using a TPA GEL EM method of a physical property tester, and recording Hardness and Chewiness.

Description

technical field [0001] The invention relates to a gel assay method, in particular to a gel assay method for soybean protein. Background technique [0002] Several common gel detection methods at present [0003] Method 1: 1. Accurately weigh 30g of protein powder, and measure 170ml of 2.5% saline (protein concentration is 15%). Slowly pour the weighed protein powder and cooling water at about 5°C into the blender and stir thoroughly for 1 minute, stop once every 20 seconds, and scrape up the protein powder on the wall and bottom of the mixing cup with a plastic shovel every time you stop to facilitate even mixing , Stir slowly at the beginning, and then stir quickly. Transfer all the homogenized proteins to a large sample bag and then vacuum. Fill the vacuumed protein into the mold. The filling process should be slow and steady to avoid air bubbles. Three parallel samples are made for each sample. [0004] 2. Heat in a water bath at 80°C for 30 minutes, then cool in runn...

AI Technical Summary

Problems solved by technology

[0014] The purpose of the present invention is to solve the problem of low pertinence of protein gel detection in existing several protein gel determination methods, poor stability of experimental detection results, cumbersome experimental scheme, low applicability, and the need for experimental personnel to operate It has high experience and other issues, and provides a new protein gel assay method, which can more effectively improve the stability of the gel, make the assay results accurate and fast, and the operation method is easy to learn, scientific and practical