Homocysteine diagnosing/measuring reagent (kit) and homocysteine concentration measuring method
A technology for homocysteine and concentration determination, which is applied in biological testing, biochemical equipment and methods, microbial measurement/inspection, etc. It can solve the problems of long test time, complicated operation, and large variation of test data.
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Embodiment 1
[0044] The homocysteine diagnosis / measurement reagent of this embodiment is a single reagent, including:
[0045] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0046] Stabilizer 500mmol / L
[0047] Coenzyme 3mmol / L
[0048] Glutathione Homocystine Transhydrogenase 10000U / L
[0049] Glutathione oxidase 12000U / L
[0050] NAD(P)H oxidase 12000U / L
[0051] Glutathione disulfide 5mmol / L
[0052] After the reagents are all dissolved and prepared, they are divided into bottles and freeze-dried to make dry powder reagents; before use, add purified water and reconstitute for use.
[0053] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance ≤ 0.1, test main wavelength 340nm, test subwavelength 405nm, volume ratio of tested homocysteine sample to reagent is 1 / 25, the reaction direction is positive reaction (rising reaction), the delay time is about 0 minutes, and the detection time is about 5 minutes.
...
Embodiment 2
[0056] The homocysteine diagnosis / measurement reagent of this embodiment is a dual reagent, including:
[0057] Reagent 1
[0058] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0059] Stabilizer 50mmol / L
[0060] Coenzyme 3mmol / L
[0061] Glutathione disulfide 5mmol / L
[0062] Reagent 2
[0063] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0064] Stabilizer 50mmol / L
[0065] Glutathione Homocystine Transhydrogenase 10000U / L
[0066] Glutathione oxidase 12000U / L
[0067] NAD(P)H oxidase 12000U / L
[0068] After the reagents are all dissolved and prepared, they are divided into bottles to make liquid double reagents, which can be used directly.
[0069] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance ≤ 0.1, test main wavelength 340nm, test subwavelength 405nm, tested homocysteine sample and reagent 1, reagent 2 The volume ratio is 2 / 20 / 5, the reaction direction i...
Embodiment 3
[0072] The homocysteine diagnosis / measurement reagents of this embodiment are three reagents, including:
[0073] Reagent 1
[0074] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0075] Stabilizer 50mmol / L
[0076] Coenzyme 3mmol / L
[0077] Glutathione disulfide 5mmol / L
[0078] Reagent 2
[0079] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0080] Stabilizer 500mmol / L
[0081] Glutathione oxidase 12000U / L
[0082] NAD(P)H oxidase 12000U / L
[0083] Reagent 3
[0084] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0085] Stabilizer 500mmol / L
[0086] Glutathione Homocystine Transhydrogenase 10000U / L
[0087] After all the reagents are dissolved and prepared, they are divided into bottles to make liquid three reagents, which can be used directly.
[0088] When measuring the concentration of homocysteine, set it on the automatic biochemical analyzer: temperature 37 ° C, reaction time 10 minutes, in...
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