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Chitosan nanofibre and preparation method and application thereof

A technology of chitosan nano and chitosan, which is applied in the fields of fiber chemical characteristics, textiles and papermaking, medical science, etc., can solve problems such as complex processes, achieve simplified process steps, good biocompatibility, and broad application prospects Effect

Inactive Publication Date: 2010-06-16
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But usually, electrospinning technology requires special equipment and complicated process

Method used

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  • Chitosan nanofibre and preparation method and application thereof
  • Chitosan nanofibre and preparation method and application thereof
  • Chitosan nanofibre and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Weigh 0.1 gram of chitosan (90% degree of deacetylation, weight average molecular weight of 50,000) and dissolve it in 100 milliliters of 0.5% acetic acid solution, and magnetically stir at normal temperature to prepare a 0.1% chitosan acetic acid solution. The chitosan solution was placed in liquid nitrogen and frozen for 24h, and then freeze-dried for 7 days at -80°C. 10) and deionized water cleaning, then freeze-drying at -80°C for 7 days to obtain chitosan nanofibers, the diameter of which is 250-400nm, such as figure 1 shown.

Embodiment 2

[0035] Weigh 0.05 gram of chitosan (degree of deacetylation 95%, weight average molecular weight is 350,000) and dissolve in 100 milliliters of 0.25% acetic acid solution, stir magnetically at normal temperature, and prepare a chitosan acetic acid solution with a concentration of 0.05%. The chitosan solution was placed in liquid nitrogen and frozen for 12 h, and then freeze-dried for 3 days at -80 ° C. The chitosan nanofibers obtained were successively washed with 0.5 mol / L NaOH ethanol aqueous solution (the volume ratio of ethanol to water was 95: 5) and deionized water cleaning, then freeze-drying at -80°C for 3 days to obtain chitosan nanofibers, with diameters between 150 and 350nm, such as figure 2 shown.

Embodiment 3

[0037] Weigh 0.01 gram of chitosan (95% degree of deacetylation, 1.5 million weight average molecular weight) and dissolve it in 100 milliliters of 0.005% hydrochloric acid solution, and magnetically stir at room temperature to prepare a 0.01% chitosan hydrochloric acid solution. The chitosan solution was frozen in liquid nitrogen for 1 min, and then freeze-dried at -80°C for 1 day to obtain nanofibers. After washing with deionized water and freeze-drying at -80°C for 1 day, chitosan nanofibers were obtained, with diameters ranging from 50 to 500 nm, such as image 3 shown.

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Abstract

The invention relates to chitosan nanofibre and a preparation method and application thereof. The preparation method comprises the following steps: (1) dissolving chitosan with acid to obtain chitosan solution; (2) performing phase separation to the chitosan solution obtained in the step (1); and (3) lyophilizing the product obtained in the step (2), washing, and then lyophilizing again to obtain the chitosan nanofibre. The chitosan-based nanofibre prepared by the method of the invention is biodegradable, and has an extracellular matrix-type structure and broad application prospects in fields of tissue engineering, clinical wound repair and the like.

Description

technical field [0001] The invention belongs to the technical field of biomedical polymer materials, and relates to a preparation method and application of biodegradable nanofibers, in particular to a chitosan nanofiber and a preparation method and application thereof. Background technique [0002] Natural polymer chitosan has low toxicity, good biocompatibility and biodegradability, and is widely used in biomedical fields. The structure of chitosan is similar to that of glycosaminoglycan in extracellular matrix (ECMs), and it can be prepared into macroscopic shapes with different microscopic topography, and has a certain mechanical strength to meet the three-dimensional requirements of tissue defect repair in different parts. The porous scaffold can be used as a three-dimensional carrier for cells and can be used in the field of tissue engineering to repair or reconstruct diseased tissues. Studies have shown that in the fields of tissue engineering and wound repair, the ap...

Claims

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Application Information

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IPC IPC(8): D01F9/00A61L27/20A61L27/50A61L31/04A61L31/14
Inventor 屠美赵剑豪曾戎查振刚陈昊东
Owner JINAN UNIVERSITY
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