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Method for preparing inactivated bivalent vaccine against rabbit hemorrhagic disease and pasteurella multocida disease

A technology of rabbit viral hemorrhage and double inactivated vaccine, which can be used in antiviral agents, viral antigen components, and medical preparations containing active ingredients, etc., and can solve problems such as threats to the healthy development of the rabbit industry and economic losses

Active Publication Date: 2010-06-16
CHINA ANIMAL HUSBANDRY IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] With the large-scale and intensified development of the rabbit industry, several other infectious diseases that hinder the development of the rabbit industry are still prevalent, such as pasteurellosis and Clostridium welchii disease, which seriously threaten the healthy development of the rabbit industry, resulting in huge economic loss

Method used

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  • Method for preparing inactivated bivalent vaccine against rabbit hemorrhagic disease and pasteurella multocida disease
  • Method for preparing inactivated bivalent vaccine against rabbit hemorrhagic disease and pasteurella multocida disease

Examples

Experimental program
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Effect test

Embodiment 1

[0043] Rabbit virus haemorrhagic disease CD 85-2 Strain subculture

[0044] The preserved rabbit viral hemorrhagic disease strain CD 85-2 Make 10-fold dilution with normal saline, and inoculate healthy antibody-negative rabbits weighing 1.5kg at the age of two months from unimmunized non-epidemic areas. Each subcutaneous injection of 1ml, the virus titer in the rabbit can be maintained at 2 10 Bacteria / ml or more. Aseptically collect rabbits with a weight of 1.5kg that died within 24-96 hours after inoculation, aseptically collect the heart, liver, lung, spleen or kidney with histopathological changes from rabbits that died within 1 hour, and remove them The connective tissue on the body, in this example, the liver is selected as the poisonous species.

[0045] CD 85-2 For inoculation, the heart, liver, lung, spleen or kidney of the dead rabbit whose connective tissue has been removed, preferably the liver is mashed with saline, and filtered to prepare a tissue emulsion w...

Embodiment 2

[0050] The preserved rabbit viral hemorrhagic disease strain CD 85-2 Make 10-fold dilution with normal saline, and inoculate 5-year-old healthy antibody-negative rabbits with a body weight of 2.0 kg from non-infested areas. Each subcutaneous injection of 1ml, the virus titer in the rabbit can be maintained at 2 10 above. Aseptically collect rabbits with a weight of 2.0kg that died within 24-96 hours after inoculation, aseptically collect the heart, liver, lung, spleen or kidney with histopathological changes from rabbits that died within 1 hour, and remove them In this case, the spleen is selected as the poisonous connective tissue.

[0051] CD 85-2 For inoculation, the heart, liver, lung, spleen or kidney of the dead rabbit whose connective tissue has been removed is selected and the spleen is mashed with normal saline, and filtered to prepare a tissue emulsion with a volume ratio of 1:9 between the mashed internal organs and normal saline. Then add formaldehyde solutions...

Embodiment 3

[0056] Vaccine Safety Test

[0057] Inoculate 1-month-old healthy susceptible rabbits with subcutaneous or intramuscular single-dose 1ml of the vaccine obtained according to the method of Example 1 and carry out safety test: each 4 healthy susceptible rabbits of 1-month-old were inoculated subcutaneously or intramuscularly with the trial-made vaccine, 1ml / only, in addition, inject 1ml / non-immune control of sterilized physiological saline to 4 rabbits at the same time, measure body temperature, observe for 10 days. Results After immunization, there was no abnormal mental state, normal appetite, no significant change in body temperature, and no adverse reactions such as inflammation at the injection site.

[0058] Safety test of subcutaneous or intramuscular single dose 1ml repeated inoculation of 1-month-old healthy susceptible rabbits: subcutaneously or intramuscularly inoculate 4 1-month-old healthy susceptible rabbits with 1ml each, and repeat the inoculation 7 days later I...

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Abstract

The invention provides a method for preparing an inactivated bivalent vaccine against a rabbit hemorrhagic disease and a pasteurella multocida disease, which comprises the following steps: inoculating a rabbit hemorrhagic disease strain CD85-2 strain to a rabbit, aseptically collecting the heart, liver, spleen, lung or kidney of the rabbit dead in 24 to 96h, and removing the connective tissues on the heart, liver, spleen, lung or kidney; preparing the mashed and filtered heart, liver, spleen, lung or kidney into an emulsion after mashing and filtering, and inactivating the emulsion; reactivating a rabbit originated capsula group A pasteurella multocida strain C51-17 strain, performing propagation culture on the reactivated strain, and inactivating the liquid C51-17 strain containing the A type pasteurella multocida strain obtained by culture; and mixing the inactivated liquid strain and an adjuvant, concentrating the mixture to obtain the liquid strain containing the A type pasteurella multocida C51-17, mixing the liquid strain and the inactivated emulsion to obtain the inactivated bivalent vaccine. The inactivated bivalent vaccine prepared by the method has the advantages of high safety, high vaccination efficacy and long shelf life.

Description

technical field [0001] The invention relates to a method for preparing a rabbit viral hemorrhagic disease and pasteurellosis multocida dual inactivated vaccine. Background technique [0002] Rabbit viral hemorrhagic disease is a severe infectious disease of rabbits that first broke out in southern Jiangsu in 1984. It mainly harms young and adult rabbits, with a mortality rate as high as 90%, but it does not cause disease to young rabbits under the age of 2 months. The pathological changes of the disease are characterized by hemorrhage in various parenchymal organs throughout the body, especially the trachea, lung, liver, and kidney. During the epidemic period of the disease, all kinds of antibiotics and sulfonamide drugs were used in the rabbit farms in the epidemic area for treatment, but none of them could reduce the morbidity and mortality. At present, a strain of rabbit viral hemorrhagic disease virus CD with good immunogenicity has been collected, isolated, identified...

Claims

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Application Information

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IPC IPC(8): A61K39/102A61K39/12A61P31/04A61P31/14
Inventor 申咏红龚文波孙勇何信群吕品王珍芹刘平严石
Owner CHINA ANIMAL HUSBANDRY IND
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