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Method for extracting alga-inhibiting active ingredients from Artemisia annua L. and alga-inhibiting method

A technology of anti-algae activity and Artemisia annua, applied in botany equipment and methods, herbicides and algicides, chemicals for biological control, etc., can solve the problems of high cost and difficult separation, and achieve low cost, The effect of easy availability of raw materials and strong environmental affinity

Inactive Publication Date: 2013-06-05
HOHAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to overcome the shortcomings of difficult separation and high cost in the actual use of existing plant algae inhibitors, the present invention provides a method for extracting algae inhibitory active ingredients from Artemisia annua and an algae inhibitory method, which have a wide range of sources and good algae inhibitory effect ,low cost

Method used

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  • Method for extracting alga-inhibiting active ingredients from Artemisia annua L. and alga-inhibiting method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] The aerial parts of Artemisia annua in flowering stage were collected, dried at 80°C for 48 hours, and crushed. Weigh 300 g of the pulverized Artemisia annua, and extract it twice with 1.5 L of petroleum ether under the assisted action of 300 W, 40 KHz ultrasonic waves, each time for 3 hours. After filtering, the petroleum ether extracts were combined, and then washed once with 1 L of 0.5-5% wt hydrochloric acid solution. The petroleum ether extract after pickling is extracted twice with 2L of 1-5% sodium hydroxide solution, the water phase is combined, and the pH is adjusted to 2-3 with concentrated hydrochloric acid or sulfuric acid, and extracted twice with 0.5L of ethyl acetate , the ethyl acetate layers were combined and concentrated to obtain 6.5 g of the algae-inhibiting active ingredient.

[0018] The residue of Artemisia annua leached with petroleum ether was extracted twice with 1.5 L of ethyl acetate, 3 hours each time, with the assistance of ultrasonic wave...

Embodiment 2

[0021] The aerial parts of Artemisia annua in flowering stage were collected, dried at 80°C for 48 hours, and crushed. Weigh 60 g of crushed Artemisia annua, extract with 350 ml of n-hexane with a Soxhlet extractor for 48 hours, and wash once with 200 ml of 0.5-5% dilute acid for the n-hexane extract. The n-hexane extract after pickling was extracted 2 to 3 times with 300ml of 1-5% dilute alkali, the water phase was combined and adjusted to pH=2 to 3, extracted twice with 200ml of ether, the ether layers were combined and concentrated to obtain pH Algae active ingredient 1.12g.

[0022] The above-mentioned Artemisia annua residue extracted by Soxhlet with n-hexane was extracted with 350 ml of ether for 48 hours. After the extraction, the ether layer was washed once with 200ml of 0.5-5% dilute acid and 2-3 times with 300ml of 1-5% dilute alkali, and then the ether layer was concentrated to obtain 1.33g of algastatic active ingredient II.

[0023] The algae-inhibiting active i...

Embodiment 3

[0025] Apply the algastatic agent obtained by the above method to carry out acute toxicity test on aseptically cultured Microcystis aeruginosa, set some concentration gradients for each drug, set blank and two groups in parallel, observe once every 24 hours, and observe continuously for 5 days. Observations are expressed as inhibition rates at 96 hours. The result is as follows:

[0026] The algae-inhibiting active ingredient 1 obtained from Experimental Example 1, when the concentration reaches 100 mg / L, its inhibition rate on algae reaches 97%.

[0027] The algae-inhibiting active ingredient 2 obtained in Experimental Example 1, when the concentration reaches 150 mg / L, its inhibition rate on algae reaches 98%.

[0028] The mixed algastatic agent obtained from Experimental Example 1: when the concentration reaches 60 mg / L, its inhibition rate on algae reaches 97%.

[0029] The mixed algastatic agent obtained from Experimental Example 2: when the concentration reaches 80 mg / ...

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Abstract

The invention discloses a method for extracting alga-inhibiting active ingredients from Artemisia annua L., and an alga-inhibiting method, and belongs to the field of water pollution control. The invention solves the problems of high cost, large ecological risk, secondary pollution and the like of the traditional alga-inhibiting technology. The device discloses a method for extracting and preparing an alga-inhibiting agent from land plant of Artemisia annua L. The method comprises the following steps of: extracting the dried and crushed Artemisia annua L. by steps with petroleum ether and ethyl acetate, washing petroleum ether extracting solution with diluted acid, combining active ingredients obtained by diluted alkaline stripping and active ingredients obtained by washing ethyl acetate extracting solution with diluted acid and diluted alkaline, and then preparing the alga-inhibiting agent. The method has the advantages of simple process, easy implementation, low cost and the like, and can be widely used for waters of ponds, reservoirs, lakes and the like.

Description

technical field [0001] The invention belongs to the field of water body pollution control, in particular to a method for extracting algae-inhibiting active ingredients from natural plant Artemisia annua. Background technique [0002] At present, the methods for controlling algal blooms in water bodies mainly include physical methods, chemical methods and biological methods, among which the most commonly used method is chemical method. [0003] The existing chemical algicides are mainly copper sulfate. The mechanism is to use the toxicity of copper ions to interfere with the photosynthetic reaction system of algae, so that it cannot carry out photosynthesis normally, and finally achieve the purpose of killing algae. However, copper ions are heavy metal ions and are not selective. They are absorbed or ingested by other aquatic plants and animals while killing algae, causing the death of other organisms in the water body, or accumulating in organisms, which will eventually caus...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N65/12A01P13/00C02F1/50
Inventor 倪利晓李时银郝向阳于东盛陈世金任高翔李晓东
Owner HOHAI UNIV
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