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Grifola frondosus liquid strain and method for cultivating grifola frondosus by using liquid strain

A technology of liquid strains and Grifola frondosa, applied in the direction of botanical equipment and methods, applications, fungal products, etc., to achieve the effects of neat fruiting, convenient factory management, and robust growth

Inactive Publication Date: 2010-06-16
SHANGHAI ACAD OF AGRI SCI +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, liquid strains have been used to cultivate mushroom species such as Flammulina velutipes, crab-flavored mushrooms, and oyster mushrooms, and industrialization has been formed in industrialized cultivation of Flammulina velutipes, but the rapid propagation technology of liquid strains has not yet been applied to industrialized cultivation of Grifola frondosa. see the report

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Production of parent species: Grifola frondosa strain block (preserved by the strain preservation center of the Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, code: GF1) was inoculated in PDA medium, and cultured in the dark at 25°C for 10 days;

[0021] First-class liquid strain production (shake flask culture method): culture solution formula: 5% potato, 2% glucose, 3% corn flour, 1% bran extract, 0.8% agar, natural pH; after the culture solution is prepared , put into a 500mL Erlenmeyer flask, the volume of each bottle is 100mL, and add 10 glass beads, add a cotton plug and then wrap it with kraft paper to seal, after sterilization, cool down to below 25°C, put it in and smash it with a homogenizer The mother species of , were cultured statically at 25°C for 48 hours, and then placed on a shaker for shaking culture at a frequency of 100r / min. After cultivating for 10 days, it can be seen that the culture solution is clear and transparent, and a...

Embodiment 2

[0026] The parent species is made the same as in Example 1; the strain used is GF2 (preserved by the Strain Collection Center of the Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, number: GF2)

[0027] First-class liquid strain system (shake flask culture method): culture solution formula: 5% potato, 2% glucose, 2% corn flour, 3% bran extract, 1% agar, natural pH. Bottling, sterilization, and inoculation were the same as in Example 1. After inoculation, they were placed on a shaking table for shaking culture, and the shaking frequency was 150 r / min. The room temperature of the shaker was controlled at 24°C. After 9 days of cultivation, the culture liquid was clear and transparent, and a large number of mycelia balls of Grifola frondosa were suspended in the liquid;

[0028] Production of secondary liquid strains (cultivation in liquid fermenters): the formulation of the culture medium, bottling, and sterilization are the same as in Example 1. After steri...

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Abstract

The invention discloses a grifola frondosus liquid strain and a method for cultivating grifola frondosus by using the liquid strain. The liquid strain has good dispersion and strong fluidity, and can quicken the growth speed of the strain and shorten the fermentation time; and bag hypha prepared from the liquid strain grows healthily, the growth of each part is uniform, the fungus age is regular, and the grown mushroom is regular and facilitates factory management.

Description

Technical field: [0001] The invention relates to the field of edible fungus cultivation, in particular to a liquid strain of Grifola frondosa and a method for cultivating Grifola frondosa with the rapid propagation technology of the liquid strain. Background technique: [0002] Cultivating edible fungi with liquid strains is a new technology that has emerged in recent years. "Liquid strains" and "solid strains" are relative terms, and refer to edible fungus strains obtained by submerged culture techniques using liquid culture media. . Compared with traditional solid strains, liquid strains can breed a large number of high-purity, high-vigority high-quality strains in a short period of time and at low cost, and can be conveniently inoculated directly into the bacteria bag. At present, liquid strains have been used to cultivate mushroom species such as Flammulina velutipes, crab-flavored mushrooms, and oyster mushrooms, and industrialization has been formed in industrialized ...

Claims

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Application Information

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IPC IPC(8): A01G1/04A01H15/00
Inventor 王瑞娟郭倩李玉张美彦
Owner SHANGHAI ACAD OF AGRI SCI
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